Kinetic models to produce an antioxidant by enzymatic hydrolysis of bovine plasma protein using a high substrate concentration

ABSTRACT: Background: The animal blood that is produced in a slaughterhouse is a potential source of inexpensive proteins used in the food industry around the world. However, 60% of it is surplus, and it ends with a negative environmental impact. Introduction: The enzymatic hydrolysis of proteins represents a good way to produce peptides with different biological activities. Methods: Enzymatic hydrolysis of bovine plasma with subtilisin at an alkaline pH and 61.5°C was performed using the pH-stat method. Experiments were conducted considering the effects of a high initial substrate concentration (So) and the enzyme/substrate ratio (E/S) minimizing the processing time necessary to obtain a specific degree of hydrolysis (DH). Results: The best conditions obtained were 42 g/L of So and 0.89 AU/g substrate of E/S until a DH of 20% in 11,1 ± 1,1 min was achieved to the tested conditions, which result in a fitted empirical polynomial equation of degree 3. Conclusion: A kinetic equation is established to relate the DH and the reaction time to a relative error of less than 5% in the fit, to obtain a good antioxidant product in an industrially interesting time. Additionally, the results suggest a good adjustment of the data with a determination coefficient (R2) of 0.9745 in validation.

• Autores: Zapata Montoya, José Edgar; Gómez Grimaldo, Nathalia Andrea; Gómez Sampedro, Leidy Johanna
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2019
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/39827
• Acceso en línea: https://hdl.handle.net/10495/39827
• Palabra clave: Antioxidantes; Antioxidants; Hidrolisados de Proteína; Protein Hydrolysates; Cinética; Kinetics; Hidrólisis enzimática; Enzymatic hydrolysis; http://aims.fao.org/aos/agrovoc/c_27512; https://id.nlm.nih.gov/mesh/D000975; https://id.nlm.nih.gov/mesh/D011492; https://id.nlm.nih.gov/mesh/D007700
• Rights: openAccess
• License: https://creativecommons.org/licenses/by/4.0/