Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to Infection
ABSTRACT: Tuberculosis (TB) is caused by Mycobacterium tuberculosis (Mtb), leading to pulmonary and extrapulmonary TB, whereby Mtb is disseminated to many other organs and tissues. Dissemination occurs early during the disease, and bacteria can be found first in the lymph nodes adjacent to the lungs...
- Autores:
-
Barrera Robledo, Luis Fernando
Alzate Restrepo, Juan Fernando
López Agudelo, Víctor Alonso
Cabarcas Jaramillo, Felipe
Barrera Enríquez, Vianey Paola
Ríos Estepa, Rigoberto
Baena García, Andrés
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2022
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/39546
- Acceso en línea:
- https://hdl.handle.net/10495/39546
- Palabra clave:
- Interacciones Huésped-Patógeno
Host-Pathogen Interactions
Mycobacterium tuberculosis
Macrófagos
Macrophages
RNA-Seq
RNA-Seq
Tuberculosis
https://id.nlm.nih.gov/mesh/D054884
https://id.nlm.nih.gov/mesh/D009169
https://id.nlm.nih.gov/mesh/D008264
https://id.nlm.nih.gov/mesh/D000081246
https://id.nlm.nih.gov/mesh/D014376
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
| id |
UDEA2_d619a1610d03d6bc9ea85135a829dc0e |
|---|---|
| oai_identifier_str |
oai:bibliotecadigital.udea.edu.co:10495/39546 |
| network_acronym_str |
UDEA2 |
| network_name_str |
Repositorio UdeA |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to Infection |
| title |
Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to Infection |
| spellingShingle |
Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to Infection Interacciones Huésped-Patógeno Host-Pathogen Interactions Mycobacterium tuberculosis Macrófagos Macrophages RNA-Seq RNA-Seq Tuberculosis https://id.nlm.nih.gov/mesh/D054884 https://id.nlm.nih.gov/mesh/D009169 https://id.nlm.nih.gov/mesh/D008264 https://id.nlm.nih.gov/mesh/D000081246 https://id.nlm.nih.gov/mesh/D014376 |
| title_short |
Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to Infection |
| title_full |
Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to Infection |
| title_fullStr |
Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to Infection |
| title_full_unstemmed |
Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to Infection |
| title_sort |
Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to Infection |
| dc.creator.fl_str_mv |
Barrera Robledo, Luis Fernando Alzate Restrepo, Juan Fernando López Agudelo, Víctor Alonso Cabarcas Jaramillo, Felipe Barrera Enríquez, Vianey Paola Ríos Estepa, Rigoberto Baena García, Andrés |
| dc.contributor.author.none.fl_str_mv |
Barrera Robledo, Luis Fernando Alzate Restrepo, Juan Fernando López Agudelo, Víctor Alonso Cabarcas Jaramillo, Felipe Barrera Enríquez, Vianey Paola Ríos Estepa, Rigoberto Baena García, Andrés |
| dc.contributor.researchgroup.spa.fl_str_mv |
Bioprocesos Grupo de Inmunología Celular e Inmunogenética Sistemas Embebidos e Inteligencia Computacional (SISTEMIC) |
| dc.subject.decs.none.fl_str_mv |
Interacciones Huésped-Patógeno Host-Pathogen Interactions Mycobacterium tuberculosis Macrófagos Macrophages RNA-Seq RNA-Seq Tuberculosis |
| topic |
Interacciones Huésped-Patógeno Host-Pathogen Interactions Mycobacterium tuberculosis Macrófagos Macrophages RNA-Seq RNA-Seq Tuberculosis https://id.nlm.nih.gov/mesh/D054884 https://id.nlm.nih.gov/mesh/D009169 https://id.nlm.nih.gov/mesh/D008264 https://id.nlm.nih.gov/mesh/D000081246 https://id.nlm.nih.gov/mesh/D014376 |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D054884 https://id.nlm.nih.gov/mesh/D009169 https://id.nlm.nih.gov/mesh/D008264 https://id.nlm.nih.gov/mesh/D000081246 https://id.nlm.nih.gov/mesh/D014376 |
| description |
ABSTRACT: Tuberculosis (TB) is caused by Mycobacterium tuberculosis (Mtb), leading to pulmonary and extrapulmonary TB, whereby Mtb is disseminated to many other organs and tissues. Dissemination occurs early during the disease, and bacteria can be found first in the lymph nodes adjacent to the lungs and then later in the extrapulmonary organs, including the spleen. The early global gene expression response of human tissue macrophages and intracellular clinical isolates of Mtb has been poorly studied. Using dual RNA-seq, we have explored the mRNA profiles of two closely related clinical strains of the Latin American and Mediterranean (LAM) family of Mtb in infected human splenic macrophages (hSMs). This work shows that these pathogens mediate a distinct host response despite their genetic similarity. Using a genome-scale host-pathogen metabolic reconstruction to analyze the data further, we highlight that the infecting Mtb strain also determines the metabolic response of both the host and pathogen. Thus, macrophage ontogeny and the genetic-derived program of Mtb direct the host-pathogen interaction. |
| publishDate |
2022 |
| dc.date.issued.none.fl_str_mv |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2024-06-02T11:25:05Z |
| dc.date.available.none.fl_str_mv |
2024-06-02T11:25:05Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
| dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| status_str |
publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
López-Agudelo VA, Baena A, Barrera V, Cabarcas F, Alzate JF, Beste DJV, Ríos-Estepa R, Barrera LF. Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to Infection. Int J Mol Sci. 2022 Feb 4;23(3):1803. doi: 10.3390/ijms23031803. |
| dc.identifier.issn.none.fl_str_mv |
1661-6596 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/39546 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/ijms23031803 |
| dc.identifier.eissn.none.fl_str_mv |
1422-0068 |
| identifier_str_mv |
López-Agudelo VA, Baena A, Barrera V, Cabarcas F, Alzate JF, Beste DJV, Ríos-Estepa R, Barrera LF. Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to Infection. Int J Mol Sci. 2022 Feb 4;23(3):1803. doi: 10.3390/ijms23031803. 1661-6596 10.3390/ijms23031803 1422-0068 |
| url |
https://hdl.handle.net/10495/39546 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Int. J. Mol. Sci. |
| dc.relation.citationendpage.spa.fl_str_mv |
22 |
| dc.relation.citationissue.spa.fl_str_mv |
3 |
| dc.relation.citationstartpage.spa.fl_str_mv |
1 |
| dc.relation.citationvolume.spa.fl_str_mv |
23 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
International Journal of Molecular Sciences |
| dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by/2.5/co/ |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/co/ https://creativecommons.org/licenses/by/4.0/ http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.extent.spa.fl_str_mv |
22 páginas |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.publisher.spa.fl_str_mv |
MDPI |
| dc.publisher.place.spa.fl_str_mv |
Basilea, Suiza |
| institution |
Universidad de Antioquia |
| bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstreams/5881a183-ba0f-4161-a456-2a38eb0d1a4c/download https://bibliotecadigital.udea.edu.co/bitstreams/2f963eaa-c0c5-4dcf-9d79-bec2ae2c0b21/download https://bibliotecadigital.udea.edu.co/bitstreams/ec1d6c68-a9c1-48c0-bc04-d2865894637c/download https://bibliotecadigital.udea.edu.co/bitstreams/91a546de-3231-4f13-9e26-c5cb15314dac/download https://bibliotecadigital.udea.edu.co/bitstreams/22571ac2-4404-4ac2-bd5f-a3ea842c145a/download |
| bitstream.checksum.fl_str_mv |
a0100fe77ec5584fea5a02d5652bcbf8 1646d1f6b96dbbbc38035efc9239ac9c 8a4605be74aa9ea9d79846c1fba20a33 72c08c650621f32c828e1992822e6520 2148698d5a6303d85a574afe5e3b82fe |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Institucional de la Universidad de Antioquia |
| repository.mail.fl_str_mv |
aplicacionbibliotecadigitalbiblioteca@udea.edu.co |
| _version_ |
1851052434406768640 |
| spelling |
Barrera Robledo, Luis FernandoAlzate Restrepo, Juan FernandoLópez Agudelo, Víctor AlonsoCabarcas Jaramillo, FelipeBarrera Enríquez, Vianey PaolaRíos Estepa, RigobertoBaena García, AndrésBioprocesosGrupo de Inmunología Celular e InmunogenéticaSistemas Embebidos e Inteligencia Computacional (SISTEMIC)2024-06-02T11:25:05Z2024-06-02T11:25:05Z2022López-Agudelo VA, Baena A, Barrera V, Cabarcas F, Alzate JF, Beste DJV, Ríos-Estepa R, Barrera LF. Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to Infection. Int J Mol Sci. 2022 Feb 4;23(3):1803. doi: 10.3390/ijms23031803.1661-6596https://hdl.handle.net/10495/3954610.3390/ijms230318031422-0068ABSTRACT: Tuberculosis (TB) is caused by Mycobacterium tuberculosis (Mtb), leading to pulmonary and extrapulmonary TB, whereby Mtb is disseminated to many other organs and tissues. Dissemination occurs early during the disease, and bacteria can be found first in the lymph nodes adjacent to the lungs and then later in the extrapulmonary organs, including the spleen. The early global gene expression response of human tissue macrophages and intracellular clinical isolates of Mtb has been poorly studied. Using dual RNA-seq, we have explored the mRNA profiles of two closely related clinical strains of the Latin American and Mediterranean (LAM) family of Mtb in infected human splenic macrophages (hSMs). This work shows that these pathogens mediate a distinct host response despite their genetic similarity. Using a genome-scale host-pathogen metabolic reconstruction to analyze the data further, we highlight that the infecting Mtb strain also determines the metabolic response of both the host and pathogen. Thus, macrophage ontogeny and the genetic-derived program of Mtb direct the host-pathogen interaction.Universidad de Antioquia. Vicerrectoría de investigación. Comité para el Desarrollo de la Investigación - CODICOL0008639COL0023715COL001071722 páginasapplication/pdfengMDPIBasilea, Suizahttp://creativecommons.org/licenses/by/2.5/co/https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to InfectionArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionInteracciones Huésped-PatógenoHost-Pathogen InteractionsMycobacterium tuberculosisMacrófagosMacrophagesRNA-SeqRNA-SeqTuberculosishttps://id.nlm.nih.gov/mesh/D054884https://id.nlm.nih.gov/mesh/D009169https://id.nlm.nih.gov/mesh/D008264https://id.nlm.nih.gov/mesh/D000081246https://id.nlm.nih.gov/mesh/D014376Int. J. Mol. Sci.223123International Journal of Molecular Sciences2014–765RoR:03bp5hc83PublicationORIGINALBaenaAndres_2022_Dual_RNA_Mycobacterium.pdfBaenaAndres_2022_Dual_RNA_Mycobacterium.pdfArtículo de investigaciónapplication/pdf4929304https://bibliotecadigital.udea.edu.co/bitstreams/5881a183-ba0f-4161-a456-2a38eb0d1a4c/downloada0100fe77ec5584fea5a02d5652bcbf8MD51trueAnonymousREADCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927https://bibliotecadigital.udea.edu.co/bitstreams/2f963eaa-c0c5-4dcf-9d79-bec2ae2c0b21/download1646d1f6b96dbbbc38035efc9239ac9cMD52falseAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/ec1d6c68-a9c1-48c0-bc04-d2865894637c/download8a4605be74aa9ea9d79846c1fba20a33MD53falseAnonymousREADTEXTBaenaAndres_2022_Dual_RNA_Mycobacterium.pdf.txtBaenaAndres_2022_Dual_RNA_Mycobacterium.pdf.txtExtracted texttext/plain97275https://bibliotecadigital.udea.edu.co/bitstreams/91a546de-3231-4f13-9e26-c5cb15314dac/download72c08c650621f32c828e1992822e6520MD54falseAnonymousREADTHUMBNAILBaenaAndres_2022_Dual_RNA_Mycobacterium.pdf.jpgBaenaAndres_2022_Dual_RNA_Mycobacterium.pdf.jpgGenerated Thumbnailimage/jpeg15930https://bibliotecadigital.udea.edu.co/bitstreams/22571ac2-4404-4ac2-bd5f-a3ea842c145a/download2148698d5a6303d85a574afe5e3b82feMD55falseAnonymousREAD10495/39546oai:bibliotecadigital.udea.edu.co:10495/395462025-03-26 22:17:31.098http://creativecommons.org/licenses/by/2.5/co/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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 |