A rapid and simple quantitative method for specific detection of smaller coterminal RNA by PCR (DeSCo-PCR): application to the detection of viral subgenomic RNAs

ABSTRACT: RNAs that are 5'-truncated versions of a longer RNA but share the same 3' terminus can be generated by alternative promoters in transcription of cellular mRNAs or by replicating RNA viruses. These truncated RNAs cannot be distinguished from the longer RNA by a simple two-primer R...

Full description

Autores:

Roa Linares, Vicky Constanza
Reddisiva Prasanth, K
Kanodia, Pulkit
Bradrick, Shelton S
Garcia-Blanco, Mariano A
Miller, W Allen

Tipo de recurso:

Article of investigation

Fecha de publicación:

2020

Institución:

Universidad de Antioquia

Repositorio:

Repositorio UdeA

Idioma:

eng

OAI Identifier:

oai:bibliotecadigital.udea.edu.co:10495/45412

Acceso en línea:

https://hdl.handle.net/10495/45412

Palabra clave:

Alternative Splicing
Empalme Alternativo
Cell Line, Tumor
Línea Celular Tumoral
DNA, Complementary
ADN Complementario
Evaluation Studies as Topic
Estudios de Evaluación como Asunto
Genome, Viral
Genoma Viral
HeLa Cells
Células HeLa
Nucleic Acid Conformation
Conformación de Ácido Nucleico
Polymerase Chain Reaction
Reacción en Cadena de la Polimerasa
Promoter Regions, Genetic
Regiones Promotoras Genéticas
RNA Viruses
Virus ARN
RNA, Messenger
ARN Mensajero
RNA, Viral
ARN Viral
Tombusviridae
Tombusviridae
Zika Virus
Virus Zika
https://id.nlm.nih.gov/mesh/D017398
https://id.nlm.nih.gov/mesh/D045744
https://id.nlm.nih.gov/mesh/D018076
https://id.nlm.nih.gov/mesh/D005069
https://id.nlm.nih.gov/mesh/D016679
https://id.nlm.nih.gov/mesh/D006367
https://id.nlm.nih.gov/mesh/D009690
https://id.nlm.nih.gov/mesh/D016133
https://id.nlm.nih.gov/mesh/D011401
https://id.nlm.nih.gov/mesh/D012328
https://id.nlm.nih.gov/mesh/D012333
https://id.nlm.nih.gov/mesh/D012367
https://id.nlm.nih.gov/mesh/D019183
https://id.nlm.nih.gov/mesh/D000071244

Rights

openAccess

License

https://creativecommons.org/licenses/by/4.0/