Obtaining of antioxidant peptide from bovine plasma hydrolysates and effect of the degree of hydrolysis on antioxidant capacity
ABSTRACT: In recent years there has been increased the interest in evaluate antioxidative bioactive peptides derived from food sources, bovine plasma have been shown to possess high antioxidant activity. Oxygen radical antioxidant capacity (ORAC) and trolox equivalent antioxidant capacity (TEAC) ass...
- Autores:
-
Gómez Sampedro, Leidy Johanna
Zapata Montoya, José Edgar
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/38244
- Acceso en línea:
- https://hdl.handle.net/10495/38244
- Palabra clave:
- Péptidos
Peptides
Antioxidantes
Antioxidants
Capacidad de Absorbancia de Radicales de Oxígeno
Oxygen Radical Absorbance Capacity
Capacidad de Absorbancia de Radicales de Oxígeno
Oxygen Radical Absorbance Capacity
Hidrólisis enzimática
Enzymatic hydrolysis
Bovine plasma
Plasma bovino
http://aims.fao.org/aos/agrovoc/c_27512
https://id.nlm.nih.gov/mesh/D010455
https://id.nlm.nih.gov/mesh/D000975
https://id.nlm.nih.gov/mesh/D000072257
https://id.nlm.nih.gov/mesh/D000072257
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc/2.5/co/
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| dc.title.spa.fl_str_mv |
Obtaining of antioxidant peptide from bovine plasma hydrolysates and effect of the degree of hydrolysis on antioxidant capacity |
| dc.title.translated.spa.fl_str_mv |
Obtención de péptidos antioxidantes de hidrolizados de plasma de bovino y efecto del grado de hidrólisis en su capacidad antioxidante |
| title |
Obtaining of antioxidant peptide from bovine plasma hydrolysates and effect of the degree of hydrolysis on antioxidant capacity |
| spellingShingle |
Obtaining of antioxidant peptide from bovine plasma hydrolysates and effect of the degree of hydrolysis on antioxidant capacity Péptidos Peptides Antioxidantes Antioxidants Capacidad de Absorbancia de Radicales de Oxígeno Oxygen Radical Absorbance Capacity Capacidad de Absorbancia de Radicales de Oxígeno Oxygen Radical Absorbance Capacity Hidrólisis enzimática Enzymatic hydrolysis Bovine plasma Plasma bovino http://aims.fao.org/aos/agrovoc/c_27512 https://id.nlm.nih.gov/mesh/D010455 https://id.nlm.nih.gov/mesh/D000975 https://id.nlm.nih.gov/mesh/D000072257 https://id.nlm.nih.gov/mesh/D000072257 |
| title_short |
Obtaining of antioxidant peptide from bovine plasma hydrolysates and effect of the degree of hydrolysis on antioxidant capacity |
| title_full |
Obtaining of antioxidant peptide from bovine plasma hydrolysates and effect of the degree of hydrolysis on antioxidant capacity |
| title_fullStr |
Obtaining of antioxidant peptide from bovine plasma hydrolysates and effect of the degree of hydrolysis on antioxidant capacity |
| title_full_unstemmed |
Obtaining of antioxidant peptide from bovine plasma hydrolysates and effect of the degree of hydrolysis on antioxidant capacity |
| title_sort |
Obtaining of antioxidant peptide from bovine plasma hydrolysates and effect of the degree of hydrolysis on antioxidant capacity |
| dc.creator.fl_str_mv |
Gómez Sampedro, Leidy Johanna Zapata Montoya, José Edgar |
| dc.contributor.author.none.fl_str_mv |
Gómez Sampedro, Leidy Johanna Zapata Montoya, José Edgar |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Nutrición y Tecnología de Alimentos Toxinología, Alternativas Terapéuticas y Alimentarias |
| dc.subject.decs.none.fl_str_mv |
Péptidos Peptides Antioxidantes Antioxidants Capacidad de Absorbancia de Radicales de Oxígeno Oxygen Radical Absorbance Capacity Capacidad de Absorbancia de Radicales de Oxígeno Oxygen Radical Absorbance Capacity |
| topic |
Péptidos Peptides Antioxidantes Antioxidants Capacidad de Absorbancia de Radicales de Oxígeno Oxygen Radical Absorbance Capacity Capacidad de Absorbancia de Radicales de Oxígeno Oxygen Radical Absorbance Capacity Hidrólisis enzimática Enzymatic hydrolysis Bovine plasma Plasma bovino http://aims.fao.org/aos/agrovoc/c_27512 https://id.nlm.nih.gov/mesh/D010455 https://id.nlm.nih.gov/mesh/D000975 https://id.nlm.nih.gov/mesh/D000072257 https://id.nlm.nih.gov/mesh/D000072257 |
| dc.subject.agrovoc.none.fl_str_mv |
Hidrólisis enzimática Enzymatic hydrolysis |
| dc.subject.proposal.spa.fl_str_mv |
Bovine plasma Plasma bovino |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_27512 |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D010455 https://id.nlm.nih.gov/mesh/D000975 https://id.nlm.nih.gov/mesh/D000072257 https://id.nlm.nih.gov/mesh/D000072257 |
| description |
ABSTRACT: In recent years there has been increased the interest in evaluate antioxidative bioactive peptides derived from food sources, bovine plasma have been shown to possess high antioxidant activity. Oxygen radical antioxidant capacity (ORAC) and trolox equivalent antioxidant capacity (TEAC) assays were compared in order to estimate the antioxidant capacity of bovine plasma hydrolysates (BPH) obtained for Alcalase® 2.4 L. The effect of the degree of hydrolysis (DH) was also evaluated for such activity. Additionally, the peptide fractions with the greatest activity were isolated by using Ultrafiltration Membranes, Ion Exchange Chromatography, and Reverse-Phase High Performance Liquid Chromatography (RP-HPLC). An increase of the degree of hydrolysis was found for these methods that correspond to an increase in the antioxidant activity; besides, the high relationship between ORAC and TEAC assays, with a correlation coefficient of 0.974, fitted a double squared model. A peptidic fraction with highest antioxidant capacity was obtained by separation processes, with values 4457.9 ± 72.5 and 1409 ± 43.41 μmolTE/g for ORAC and TEAC methods respectively, and contained peptides with sequences Q(K)Q(K)PVRDQ(K) and GAHQPSG, which were determined by MALDI-TOF-TOF and manual nESI-MS/MS. |
| publishDate |
2016 |
| dc.date.issued.none.fl_str_mv |
2016 |
| dc.date.accessioned.none.fl_str_mv |
2024-02-20T02:46:02Z |
| dc.date.available.none.fl_str_mv |
2024-02-20T02:46:02Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
| dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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1665-2738 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/38244 |
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2395-8472 |
| identifier_str_mv |
1665-2738 2395-8472 |
| url |
https://hdl.handle.net/10495/38244 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Mex. Ing. Quim. |
| dc.relation.citationendpage.spa.fl_str_mv |
109 |
| dc.relation.citationissue.spa.fl_str_mv |
1 |
| dc.relation.citationstartpage.spa.fl_str_mv |
101 |
| dc.relation.citationvolume.spa.fl_str_mv |
15 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Revista Mexicana de Ingeniera Química |
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http://creativecommons.org/licenses/by-nc/2.5/co/ |
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https://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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Universidad Autónoma Metropolitana, Unidad Iztapalapa, División de Ciencias Básicas e Ingeniería |
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Ciudad de México, México |
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Universidad de Antioquia |
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Gómez Sampedro, Leidy JohannaZapata Montoya, José EdgarGrupo de Nutrición y Tecnología de AlimentosToxinología, Alternativas Terapéuticas y Alimentarias2024-02-20T02:46:02Z2024-02-20T02:46:02Z20161665-2738https://hdl.handle.net/10495/382442395-8472ABSTRACT: In recent years there has been increased the interest in evaluate antioxidative bioactive peptides derived from food sources, bovine plasma have been shown to possess high antioxidant activity. Oxygen radical antioxidant capacity (ORAC) and trolox equivalent antioxidant capacity (TEAC) assays were compared in order to estimate the antioxidant capacity of bovine plasma hydrolysates (BPH) obtained for Alcalase® 2.4 L. The effect of the degree of hydrolysis (DH) was also evaluated for such activity. Additionally, the peptide fractions with the greatest activity were isolated by using Ultrafiltration Membranes, Ion Exchange Chromatography, and Reverse-Phase High Performance Liquid Chromatography (RP-HPLC). An increase of the degree of hydrolysis was found for these methods that correspond to an increase in the antioxidant activity; besides, the high relationship between ORAC and TEAC assays, with a correlation coefficient of 0.974, fitted a double squared model. A peptidic fraction with highest antioxidant capacity was obtained by separation processes, with values 4457.9 ± 72.5 and 1409 ± 43.41 μmolTE/g for ORAC and TEAC methods respectively, and contained peptides with sequences Q(K)Q(K)PVRDQ(K) and GAHQPSG, which were determined by MALDI-TOF-TOF and manual nESI-MS/MS.RESUMEN: En los últimos años se ha incrementado el interés en evaluar péptidos bioactivos antioxidantes derivados de fuentes alimentarias, el plasma bovino ha demostrado que poseen una alta actividad antioxidante. Se analizó la capacidad antioxidante de radicales oxígeno (ORAC) y la capacidad antioxidante equivalente a trolox (TEAC) de los hidrolizados de plasma bovino obtenidos por Alcalasa® 2.4 L y el efecto del grado de hidrólisis sobre cada actividad. Adicionalmente las fracciones peptídicas con mayor actividad fueron aisladas por medio de ultrafiltración por membranas, cromatografía de intercambio iónico y cromatografía líquida de alta eficiencia (HPLC) en fase reversa. Se encontró que un incremento en el grado de hidrólisis corresponde un incremento en la actividad antioxidante, además, se encontró una alta relación entre los métodos ORAC y TEAC, con un coeficiente de correlación de 0.974, ajustado a un modelo doble cuadrado. Finalmente una fracción peptıdica con alta capacidad antioxidante fue obtenida por medio de los procesos de separación, con valores de 4457.9 ± 72.5 y 1409 ± 43.41 μmol TE/g para los métodos de ORAC and TEAC respectivamente, la cual contenía péptidos con secuencias Q(K)Q(K)PVRDQ(K) y GAHQPSG, determinadas por MALDI-TOF-TOF y nESI-MS/MS manual.Universidad de Antioquia. Vicerrectoría de investigación. Comité para el Desarrollo de la Investigación - CODICOL0010771COL00144769 páginasapplication/pdfengUniversidad Autónoma Metropolitana, Unidad Iztapalapa, División de Ciencias Básicas e IngenieríaCiudad de México, Méxicohttp://creativecommons.org/licenses/by-nc/2.5/co/https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Obtaining of antioxidant peptide from bovine plasma hydrolysates and effect of the degree of hydrolysis on antioxidant capacityObtención de péptidos antioxidantes de hidrolizados de plasma de bovino y efecto del grado de hidrólisis en su capacidad antioxidanteArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPéptidosPeptidesAntioxidantesAntioxidantsCapacidad de Absorbancia de Radicales de OxígenoOxygen Radical Absorbance CapacityCapacidad de Absorbancia de Radicales de OxígenoOxygen Radical Absorbance CapacityHidrólisis enzimáticaEnzymatic hydrolysisBovine plasmaPlasma bovinohttp://aims.fao.org/aos/agrovoc/c_27512https://id.nlm.nih.gov/mesh/D010455https://id.nlm.nih.gov/mesh/D000975https://id.nlm.nih.gov/mesh/D000072257https://id.nlm.nih.gov/mesh/D000072257Rev. Mex. Ing. 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