Obtaining of antioxidant peptide from bovine plasma hydrolysates and effect of the degree of hydrolysis on antioxidant capacity

ABSTRACT: In recent years there has been increased the interest in evaluate antioxidative bioactive peptides derived from food sources, bovine plasma have been shown to possess high antioxidant activity. Oxygen radical antioxidant capacity (ORAC) and trolox equivalent antioxidant capacity (TEAC) assays were compared in order to estimate the antioxidant capacity of bovine plasma hydrolysates (BPH) obtained for Alcalase® 2.4 L. The effect of the degree of hydrolysis (DH) was also evaluated for such activity. Additionally, the peptide fractions with the greatest activity were isolated by using Ultrafiltration Membranes, Ion Exchange Chromatography, and Reverse-Phase High Performance Liquid Chromatography (RP-HPLC). An increase of the degree of hydrolysis was found for these methods that correspond to an increase in the antioxidant activity; besides, the high relationship between ORAC and TEAC assays, with a correlation coefficient of 0.974, fitted a double squared model. A peptidic fraction with highest antioxidant capacity was obtained by separation processes, with values 4457.9 ± 72.5 and 1409 ± 43.41 μmolTE/g for ORAC and TEAC methods respectively, and contained peptides with sequences Q(K)Q(K)PVRDQ(K) and GAHQPSG, which were determined by MALDI-TOF-TOF and manual nESI-MS/MS.

• Autores: Gómez Sampedro, Leidy Johanna; Zapata Montoya, José Edgar
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2016
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/38244
• Acceso en línea: https://hdl.handle.net/10495/38244
• Palabra clave: Péptidos; Peptides; Antioxidantes; Antioxidants; Capacidad de Absorbancia de Radicales de Oxígeno; Oxygen Radical Absorbance Capacity; Capacidad de Absorbancia de Radicales de Oxígeno; Oxygen Radical Absorbance Capacity; Hidrólisis enzimática; Enzymatic hydrolysis; Bovine plasma; Plasma bovino; http://aims.fao.org/aos/agrovoc/c_27512; https://id.nlm.nih.gov/mesh/D010455; https://id.nlm.nih.gov/mesh/D000975; https://id.nlm.nih.gov/mesh/D000072257; https://id.nlm.nih.gov/mesh/D000072257
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc/2.5/co/