Germinal center responses to SARS-CoV-2 mRNA vaccines in healthy and immunocompromised individuals

ABSTRACT: Vaccine-mediated immunity often relies on the generation of protective antibodies and memory B cells, which commonly stem from germinal center (GC) reactions. An in-depth comparison of the GC responses elicited by SARS-CoV-2 mRNA vaccines in healthy and immunocompromised individuals has not yet been performed due to the challenge of directly probing human lymph nodes. Herein, through a fine-needle aspiration-based approach, we profiled the immune responses to SARS-CoV-2 mRNA vaccines in lymph nodes of healthy individuals and kidney transplant recipients (KTXs). We found that, unlike healthy subjects, KTXs presented deeply blunted SARS-CoV-2-specific GC B cell responses coupled with severely hindered T follicular helper cell, SARS-CoV-2 receptor binding domain-specific memory B cell, and neutralizing antibody responses. KTXs also displayed reduced SARS-CoV-2-specific CD4 and CD8 T cell frequencies. Broadly, these data indicate impaired GC-derived immunity in immunocompromised individuals and suggest a GC origin for certain humoral and memory B cell responses following mRNA vaccination.

• Autores: Castaño Monsalve, Diana María; Lederer, Katlyn; Bettini, Emily; Parvathaneni, Kalpana; Painter, Mark M.; Agarwal, Divyansh; Lundgreen, Kendall A.; Weirick, Madison; Muralidharan, Kavitha; Goel, Rishi R.; Xu, Xiaoming; Drapeau, Elizabeth M.; Gouma, Sigrid; Ort, Jordan T.; Awofolaju, Moses; Greenplate, Allison R.; Coz, Carole Le; Romberg, Neil; Trofe Clark, Jennifer; Malat, Gregory; Locci, Michela
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2022
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/40181
• Acceso en línea: https://hdl.handle.net/10495/40181
• Palabra clave: SARS-CoV-2; Células T Auxiliares Foliculares; T Follicular Helper Cells; Biopsia con Aguja Fina; Biopsy, Fine-Needle; Huésped Inmunocomprometido; Immunocompromised Host; Centro Germinal; Germinal Center; Células B de Memoria; Memory B Cells; Anticuerpos Neutralizantes; Antibodies, Neutralizing; Vacunas de ARNm; mRNA Vaccines; https://id.nlm.nih.gov/mesh/D000086402; https://id.nlm.nih.gov/mesh/D000084522; https://id.nlm.nih.gov/mesh/D044963; https://id.nlm.nih.gov/mesh/D016867; https://id.nlm.nih.gov/mesh/D018858; https://id.nlm.nih.gov/mesh/D000091245; https://id.nlm.nih.gov/mesh/D057134; https://id.nlm.nih.gov/mesh/D000087503
• Rights: openAccess
• License: https://creativecommons.org/licenses/by/4.0/