Real-time PCR detection of Plasmodium directly from whole blood and filter paper samples

ABSTRACT: Background: Real-time PCR is a sensitive and specific method for the analysis of Plasmodium DNA. However, prior purification of genomic DNA from blood is necessary since PCR inhibitors and quenching of fluorophores from blood prevent efficient amplification and detection of PCR products. M...

Full description

Autores:

Arango Flórez, Eliana María
Agudelo García, Olga María
Maestre Buitrago, Amanda Elena
Yanow, Stephanie K
Martin, Kimberly A
Taylor, Brian J

Tipo de recurso:

Article of investigation

Fecha de publicación:

2011

Institución:

Universidad de Antioquia

Repositorio:

Repositorio UdeA

Idioma:

eng

OAI Identifier:

oai:bibliotecadigital.udea.edu.co:10495/34773

Acceso en línea:

https://hdl.handle.net/10495/34773

Palabra clave:

Sangre - parasitología
Blood - parasitology
Técnicas de Laboratorio Clínico - métodos
Clinical Laboratory Techniques - methods
ADN Protozoario
DNA, Protozoan
Malaria
Parasitemia
Plasmodium
Reacción en Cadena en Tiempo Real de la Polimerasa - métodos
Real-Time Polymerase Chain Reaction - methods

Rights

openAccess

License

http://creativecommons.org/licenses/by/2.5/co/