Amaryllidaceae alkaloids in skin cancer management: Photoprotective effect on human keratinocytes and anti-proliferative activity in melanoma cells

ABSTRACT: Skin cancer has high rates of mortality and therapeutic failure. In this study, to develop a multi-agent strategy for skin cancer management, the selective cytotoxicity of several alkaloid fractions and pure alkaloids isolated from Amaryllidaceae species was evaluated in melanoma cells. In addition, UVB-stimulated keratinocytes (HaCaT) were exposed to seven alkaloid fractions characterized by GC-MS, and the production of intracellular reactive oxygen species (ROS) and IL-6, were measured to evaluate their photoprotection effects. The Eucharis caucana (bulb) alkaloid fraction (20 μg/ml) had a clear effect on the viability of melanoma cells, reducing it by 45.7% without affecting healthy keratinocytes. This alkaloid fraction and tazettine (both at 2.5 μg/ml) suppressed UVB-induced ROS production by 31.6% and 29.4%, respectively. The highest anti-inflammatory potential was shown by the Zephyranthes carinata (bulb) alkaloid fraction (10 μg/ml), which reduced IL-6 production by 90.8%. According to the chemometric analysis, lycoramine and tazettine had a photoprotective effect on the UVB-exposed HaCaT cells, attenuating the production of ROS and IL-6. These results suggest that Amaryllidaceae alkaloids have photoprotective and therapeutic potential in skin cancer management, especially at low concentrations.

• Autores: Castañeda Aedo, Carol Melissa; Bravo Muñoz, Elizabeth; Cortés Rendón, Natalie Charlotte; Bedoya Bedoya, Janeth Eliana; Osorio Durango, Edison; Bastida Armengol, JaumeKarent; Borges, Warley De Souze
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2023
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/42241
• Acceso en línea: https://hdl.handle.net/10495/42241
• Palabra clave: Alcaloides - farmacología; Alkaloids - pharmacology; Alcaloides de Amaryllidaceae - farmacología; Amaryllidaceae Alkaloids - pharmacology; Interleucina-6; Interleukin-6; Queratinocitos; Keratinocytes; Melanoma - tratamiento farmacológico; Melanoma - drug therapy; Especies Reactivas de Oxígeno - farmacología; Reactive Oxygen Species - pharmacology; Neoplasias Cutáneas - tratamiento farmacológico; Skin Neoplasms - drug therapy; https://id.nlm.nih.gov/mesh/D000470; https://id.nlm.nih.gov/mesh/D047151; https://id.nlm.nih.gov/mesh/D015850; https://id.nlm.nih.gov/mesh/D015603; https://id.nlm.nih.gov/mesh/D008545; https://id.nlm.nih.gov/mesh/D017382; https://id.nlm.nih.gov/mesh/D012878
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-nd/2.5/co/