Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño

64 páginas

Autores:: Suárez Ramírez, Viviana

Tipo de recurso:

Fecha de publicación:: 2007

Institución:: Universidad de la Sabana

Repositorio:: Repositorio Universidad de la Sabana

Idioma:: spa

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dc.title.es_CO.fl_str_mv	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño
title	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño
spellingShingle	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño Bacterias Bacteriología del queso Bacteriología agrícola Enzimas digestivas
title_short	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño
title_full	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño
title_fullStr	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño
title_full_unstemmed	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño
title_sort	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño
dc.creator.fl_str_mv	Suárez Ramírez, Viviana
dc.contributor.advisor.none.fl_str_mv	Cueto Vigil, María Clementina
dc.contributor.author.none.fl_str_mv	Suárez Ramírez, Viviana
dc.subject.es_CO.fl_str_mv	Bacterias Bacteriología del queso Bacteriología agrícola Enzimas digestivas
topic	Bacterias Bacteriología del queso Bacteriología agrícola Enzimas digestivas
description	64 páginas
publishDate	2007
dc.date.created.none.fl_str_mv	2007
dc.date.issued.none.fl_str_mv	2007
dc.date.accessioned.none.fl_str_mv	2012-12-06T21:05:14Z
dc.date.available.none.fl_str_mv	2012-12-06T21:05:14Z
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dc.type.local.none.fl_str_mv	Tesis de pregrado
dc.type.hasVersion.none.fl_str_mv	publishedVersion
dc.identifier.citation.none.fl_str_mv	Ausubel, F., Brent R., Kingston R., Seidman, J.G., 2005.Current Protocols in Molecular Biology, Volume 2, John Wiley & Sons. Cruz, J. G., 2006. Aislamiento y Caracterizacion Bioquimica de las Levaduras y de las Bacterias Acido lácticas Productoras de Sustancias Antimicrobianas de Interes Industrial Presentes en el ¿Suero Costeño¿. Tesis de Grado de Ingeniería de Producción Agroindustrial, Universidad de La Sabana. Jang,J., 2002. Development of 16S rDNA PCR-PFLP for Identification of Leuconostoc and Weisella species. Kim, B., Lee, J., Jang,J., Kim,J., Hang, H., 2003. Leuonostoc inhae sp. nov., a lactic acid bacterium isolated from kimchi. Lewin, B., 2001. Genes VII, Marbán, Madrid. Loessner, M., Guenther, S., Steffan, S. and Scherer, S. 2003.A Pediocin-Producing Lactobacillus plantarum Strain Inhibits Listeria monocytogenes in a Multispecies Cheese Surface Microbial Ripening Consortium.Applied and Environmental Microbiology. 69:1854¿1857 Lopez, I., Ruiz-Larrea, F., Cocolin, L., Orr, E., Phister, T., Marshall, M., Vandergheynst, J., Mills, D.A., 2003.Design and evaluation of PCR Primers for Analysis of Bacterial Populations in Wine by Denaturing Gradient Gel Electrophoresis. Manachini, P.L.,and C. Parini. 1983. DNA restriction endonuclease cleavage patterns , DNA sequence similarity and phentotypical characteristics in some strains of Lactobacillus helveticus and Lactobacillus jugurti. Antoine van Leeuwenhoek 49:143 -152 Moreira, J.L., Mota,R., Horta, M., Teixeira, S,MR., Neumann,E., Nicoli, J., R., Nunes, A.,C. 2005. Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling. Nigatu, A. 2000. Evaluation of Numerical Analyses of RAPD and API 50 CH Patterns to Differentiate Lactobacillus plantarum,Lact. fermentum, Lact. sake, Lact. parabuchneri, Lact. gallinarum, Lact. casei, Weisella minor and related taxa Isolated from Kocho and Tef. Persing, D.H., Tenover, F.C., 2004.Molecular Microbiology, Diagnostic Principles and Practice, ASM Press, Washington D.C. Randazzo, C.L., Torriani, S., Akkermans, A.D.L., De Vos, W., Vaughan, E., 2002.Diversity, Dynamics, and Activity of Bacterial Communities during Production of an Artisanal Cheese as Evaluated by 16S rRNA Analysis. Salminen, S., Von Wright, A.,Ouwehand, A., 2004.Lactic Acid Bacteria, Microbiological and Functional Aspects, Third Edition, Marcel Dekker Inc. Swaminathan B., Matar, G.M., 1993.Molecular Typing Methods, Diagnostic Moleculas Microbiology, ASM Press, Washington D.C. Tannock, G.W., Tilsala-Timisjarvi, A, Rodtong, S. Ng, J., Munro, K.,Alatossava, T. 1999. Identification of Lactobacillus Isolates from the Gastrointestinal Tract,Silage, and Yoghurt by 16S- 23S rRNA Gene Intergenic Spacer Region Sequence Comparisons. Torriani, S., Zapparoli, G., Dellagio, F., 1999. Use of PCR-Based Methods for Rapid Differentiation of Lactobacillus delbrueckii sups. bulgaricus and L. delbrueckii subsp. lactis. Zhong, W., Millsap, K., Bialowska-Hobrzanska, H. Reid,G., 1998.Differentiation of Lactobacillus Species by Molecular Typing. Rivera, O., Maldonado, I. Universidad de Puerto Rico. Curso Biología General, Laboratorio 12. En: http://www.uprm.edu/biology/cursos/biologiageneral/EnzimasDNA.htm> [ Consulta: Septiembre 2006 ] http://industry.biomerieux-usa.com/industry/food/api/index.htm [ Consulta: Septiembre 2006 ] http://www.promega.com/catalog/country_select.asp?/enotes/applications/ap0063.htm&ckt=2 http://www.ncbi.nlm.nih.gov/ [ Consulta: Enero 2006 ] http://tools.neb.com/REBsites/index.php3 [ Consulta: Febrero 2006 ] http://www.idtdna.com/ [ Consulta: Enero 2006 ]
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identifier_str_mv	Ausubel, F., Brent R., Kingston R., Seidman, J.G., 2005.Current Protocols in Molecular Biology, Volume 2, John Wiley & Sons. Cruz, J. G., 2006. Aislamiento y Caracterizacion Bioquimica de las Levaduras y de las Bacterias Acido lácticas Productoras de Sustancias Antimicrobianas de Interes Industrial Presentes en el ¿Suero Costeño¿. Tesis de Grado de Ingeniería de Producción Agroindustrial, Universidad de La Sabana. Jang,J., 2002. Development of 16S rDNA PCR-PFLP for Identification of Leuconostoc and Weisella species. Kim, B., Lee, J., Jang,J., Kim,J., Hang, H., 2003. Leuonostoc inhae sp. nov., a lactic acid bacterium isolated from kimchi. Lewin, B., 2001. Genes VII, Marbán, Madrid. Loessner, M., Guenther, S., Steffan, S. and Scherer, S. 2003.A Pediocin-Producing Lactobacillus plantarum Strain Inhibits Listeria monocytogenes in a Multispecies Cheese Surface Microbial Ripening Consortium.Applied and Environmental Microbiology. 69:1854¿1857 Lopez, I., Ruiz-Larrea, F., Cocolin, L., Orr, E., Phister, T., Marshall, M., Vandergheynst, J., Mills, D.A., 2003.Design and evaluation of PCR Primers for Analysis of Bacterial Populations in Wine by Denaturing Gradient Gel Electrophoresis. Manachini, P.L.,and C. Parini. 1983. DNA restriction endonuclease cleavage patterns , DNA sequence similarity and phentotypical characteristics in some strains of Lactobacillus helveticus and Lactobacillus jugurti. Antoine van Leeuwenhoek 49:143 -152 Moreira, J.L., Mota,R., Horta, M., Teixeira, S,MR., Neumann,E., Nicoli, J., R., Nunes, A.,C. 2005. Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling. Nigatu, A. 2000. Evaluation of Numerical Analyses of RAPD and API 50 CH Patterns to Differentiate Lactobacillus plantarum,Lact. fermentum, Lact. sake, Lact. parabuchneri, Lact. gallinarum, Lact. casei, Weisella minor and related taxa Isolated from Kocho and Tef. Persing, D.H., Tenover, F.C., 2004.Molecular Microbiology, Diagnostic Principles and Practice, ASM Press, Washington D.C. Randazzo, C.L., Torriani, S., Akkermans, A.D.L., De Vos, W., Vaughan, E., 2002.Diversity, Dynamics, and Activity of Bacterial Communities during Production of an Artisanal Cheese as Evaluated by 16S rRNA Analysis. Salminen, S., Von Wright, A.,Ouwehand, A., 2004.Lactic Acid Bacteria, Microbiological and Functional Aspects, Third Edition, Marcel Dekker Inc. Swaminathan B., Matar, G.M., 1993.Molecular Typing Methods, Diagnostic Moleculas Microbiology, ASM Press, Washington D.C. Tannock, G.W., Tilsala-Timisjarvi, A, Rodtong, S. Ng, J., Munro, K.,Alatossava, T. 1999. Identification of Lactobacillus Isolates from the Gastrointestinal Tract,Silage, and Yoghurt by 16S- 23S rRNA Gene Intergenic Spacer Region Sequence Comparisons. Torriani, S., Zapparoli, G., Dellagio, F., 1999. Use of PCR-Based Methods for Rapid Differentiation of Lactobacillus delbrueckii sups. bulgaricus and L. delbrueckii subsp. lactis. Zhong, W., Millsap, K., Bialowska-Hobrzanska, H. Reid,G., 1998.Differentiation of Lactobacillus Species by Molecular Typing. Rivera, O., Maldonado, I. Universidad de Puerto Rico. Curso Biología General, Laboratorio 12. En: http://www.uprm.edu/biology/cursos/biologiageneral/EnzimasDNA.htm> [ Consulta: Septiembre 2006 ] 88366 TE03966
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dc.publisher.none.fl_str_mv	Universidad de la Sabana
dc.publisher.program.none.fl_str_mv	Ingeniería de Producción Agroindustrial
dc.publisher.department.none.fl_str_mv	Facultad de ingeniería
publisher.none.fl_str_mv	Universidad de la Sabana
dc.source.none.fl_str_mv	Intellectum Repositorio Universidad de la Sabana Universidad de la Sabana
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spelling	Cueto Vigil, María ClementinaSuárez Ramírez, VivianaIngeniero de Producción Agroindustrial2012-12-06T21:05:14Z2012-12-06T21:05:14Z20072007Ausubel, F., Brent R., Kingston R., Seidman, J.G., 2005.Current Protocols in Molecular Biology, Volume 2, John Wiley & Sons.Cruz, J. G., 2006. Aislamiento y Caracterizacion Bioquimica de las Levaduras y de las Bacterias Acido lácticas Productoras de Sustancias Antimicrobianas de Interes Industrial Presentes en el ¿Suero Costeño¿. Tesis de Grado de Ingeniería de Producción Agroindustrial, Universidad de La Sabana.Jang,J., 2002. Development of 16S rDNA PCR-PFLP for Identification of Leuconostoc and Weisella species.Kim, B., Lee, J., Jang,J., Kim,J., Hang, H., 2003. Leuonostoc inhae sp. nov., a lactic acid bacterium isolated from kimchi.Lewin, B., 2001. Genes VII, Marbán, Madrid.Loessner, M., Guenther, S., Steffan, S. and Scherer, S. 2003.A Pediocin-Producing Lactobacillus plantarum Strain Inhibits Listeria monocytogenes in a Multispecies Cheese Surface Microbial Ripening Consortium.Applied and Environmental Microbiology. 69:1854¿1857Lopez, I., Ruiz-Larrea, F., Cocolin, L., Orr, E., Phister, T., Marshall, M., Vandergheynst, J., Mills, D.A., 2003.Design and evaluation of PCR Primers for Analysis of Bacterial Populations in Wine by Denaturing Gradient Gel Electrophoresis.Manachini, P.L.,and C. Parini. 1983. DNA restriction endonuclease cleavage patterns , DNA sequence similarity and phentotypical characteristics in some strains of Lactobacillus helveticus and Lactobacillus jugurti. Antoine van Leeuwenhoek 49:143 -152Moreira, J.L., Mota,R., Horta, M., Teixeira, S,MR., Neumann,E., Nicoli, J., R., Nunes, A.,C. 2005. Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling.Nigatu, A. 2000. Evaluation of Numerical Analyses of RAPD and API 50 CH Patterns to Differentiate Lactobacillus plantarum,Lact. fermentum, Lact. sake, Lact. parabuchneri, Lact. gallinarum, Lact. casei, Weisella minor and related taxa Isolated from Kocho and Tef.Persing, D.H., Tenover, F.C., 2004.Molecular Microbiology, Diagnostic Principles and Practice, ASM Press, Washington D.C.Randazzo, C.L., Torriani, S., Akkermans, A.D.L., De Vos, W., Vaughan, E., 2002.Diversity, Dynamics, and Activity of Bacterial Communities during Production of an Artisanal Cheese as Evaluated by 16S rRNA Analysis.Salminen, S., Von Wright, A.,Ouwehand, A., 2004.Lactic Acid Bacteria, Microbiological and Functional Aspects, Third Edition, Marcel Dekker Inc.Swaminathan B., Matar, G.M., 1993.Molecular Typing Methods, Diagnostic Moleculas Microbiology, ASM Press, Washington D.C.Tannock, G.W., Tilsala-Timisjarvi, A, Rodtong, S. Ng, J., Munro, K.,Alatossava, T. 1999. Identification of Lactobacillus Isolates from the Gastrointestinal Tract,Silage, and Yoghurt by 16S- 23S rRNA Gene Intergenic Spacer Region Sequence Comparisons.Torriani, S., Zapparoli, G., Dellagio, F., 1999. Use of PCR-Based Methods for Rapid Differentiation of Lactobacillus delbrueckii sups. bulgaricus and L. delbrueckii subsp. lactis.Zhong, W., Millsap, K., Bialowska-Hobrzanska, H. Reid,G., 1998.Differentiation of Lactobacillus Species by Molecular Typing.Rivera, O., Maldonado, I. Universidad de Puerto Rico. Curso Biología General, Laboratorio 12. En: http://www.uprm.edu/biology/cursos/biologiageneral/EnzimasDNA.htm> [ Consulta: Septiembre 2006 ]http://industry.biomerieux-usa.com/industry/food/api/index.htm [ Consulta: Septiembre 2006 ]http://www.promega.com/catalog/country_select.asp?/enotes/applications/ap0063.htm&ckt=2http://www.ncbi.nlm.nih.gov/ [ Consulta: Enero 2006 ]http://tools.neb.com/REBsites/index.php3 [ Consulta: Febrero 2006 ]http://www.idtdna.com/ [ Consulta: Enero 2006 ]http://hdl.handle.net/10818/474888366TE0396664 páginasEn la etapa previa de este proyecto se identificaron 31 de 40 aislamientos de bacterias ácido lácticas encontradas en muestras de suero costeño; dicha identificación se realizó usando la fermentación diferencial de azucares de cada bacteria. En este estudio se extrajo y purificó el ADN total de cada cepa a partir del cultivo fresco de cada uno de las cepas haciendo uso de un estuche comercial y se utilizaron unas cepas de control. Se estandarizó la técnica de PCR amplificando dos regiones de la subunidad 16S ribosomal y se realizó digestión enzimática a los fragmentos amplificados con dos enzimas de restricción. Los resultados confirmaron la identificación previa para algunas cepas, y mostraron posible variación genética de especie para otras.Universidad de la SabanaIngeniería de Producción AgroindustrialFacultad de ingenieríaIntellectum Repositorio Universidad de la SabanaUniversidad de la SabanaBacteriasBacteriología del quesoBacteriología agrícolaEnzimas digestivasCaracterización molecular de bacterias ácido lácticas aisladas de suero costeñoThesisinfo:eu-repo/semantics/bachelorThesisTesis de pregradopublishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_7a1fspahttp://purl.org/coar/access_right/c_16ecPublicationORIGINAL130510.pdf130510.pdfVer documento en PDFapplication/pdf912669https://dspace-unisabana.metabuscador.org/bitstreams/7bdf9f5f-da20-52b7-e053-7e0910accd73/download0cf252fa97b0eb5ec443e43ca9d50b70MD51trueAdministratorREADLICENSElicense.txtlicense.txttext/plain; charset=utf-8498https://dspace-unisabana.metabuscador.org/bitstreams/7bdf9f5f-da22-52b7-e053-7e0910accd73/downloadf52a2cfd4df262e08e9b300d62c85cabMD52falseAdministratorREADTEXT130510.pdf.txt130510.pdf.txtExtracted texttext/plain100693https://dspace-unisabana.metabuscador.org/bitstreams/7bdf9f5f-dfd0-52b7-e053-7e0910accd73/download9be909ba272f6b171cda9e4f3a2bfcf9MD53falseAdministratorREADTHUMBNAIL130510.pdf.jpg130510.pdf.jpgGenerated Thumbnailimage/jpeg5900https://dspace-unisabana.metabuscador.org/bitstreams/a594763d-06ed-4496-bb35-f6c51e376770/downloadf50278abe9954f4b3f157a5fb3de1844MD54falseAdministratorREAD10818/4748oai:dspace-unisabana.metabuscador.org:10818/47482025-08-09 04:38:32.919restrictedhttps://dspace-unisabana.metabuscador.orgIntellectum Repositorio Universidad de La Sabanacontactointellectum@unisabana.edu.co

Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño

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