Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño

64 páginas

Autores:

Tipo de recurso:

Fecha de publicación:: 2007

Institución:: Universidad de la Sabana

Repositorio:: Repositorio Universidad de la Sabana

Idioma:: spa

id	REPOUSABAN_fe8965e7c82f2383e3946cbafa14d01f
oai_identifier_str	oai:intellectum.unisabana.edu.co:10818/4748
network_acronym_str	REPOUSABAN
network_name_str	Repositorio Universidad de la Sabana
repository_id_str
dc.title.none.fl_str_mv	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño
title	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño
spellingShingle	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño Bacterias Bacteriología del queso Bacteriología agrícola Enzimas digestivas
title_short	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño
title_full	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño
title_fullStr	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño
title_full_unstemmed	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño
title_sort	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño
dc.contributor.none.fl_str_mv	Cueto Vigil, María Clementina
dc.subject.none.fl_str_mv	Bacterias Bacteriología del queso Bacteriología agrícola Enzimas digestivas
topic	Bacterias Bacteriología del queso Bacteriología agrícola Enzimas digestivas
description	64 páginas
publishDate	2007
dc.date.none.fl_str_mv	2007 2007 2012-12-06T21:05:14Z 2012-12-06T21:05:14Z
dc.type.none.fl_str_mv	Tesis/Trabajo de grado - Pregrado publishedVersion http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 Texto info:eu-repo/semantics/bachelorThesis http://purl.org/redcol/resource_type/TP
dc.identifier.none.fl_str_mv	Ausubel, F., Brent R., Kingston R., Seidman, J.G., 2005.Current Protocols in Molecular Biology, Volume 2, John Wiley & Sons. Cruz, J. G., 2006. Aislamiento y Caracterizacion Bioquimica de las Levaduras y de las Bacterias Acido lácticas Productoras de Sustancias Antimicrobianas de Interes Industrial Presentes en el ¿Suero Costeño¿. Tesis de Grado de Ingeniería de Producción Agroindustrial, Universidad de La Sabana. Jang,J., 2002. Development of 16S rDNA PCR-PFLP for Identification of Leuconostoc and Weisella species. Kim, B., Lee, J., Jang,J., Kim,J., Hang, H., 2003. Leuonostoc inhae sp. nov., a lactic acid bacterium isolated from kimchi. Lewin, B., 2001. Genes VII, Marbán, Madrid. Loessner, M., Guenther, S., Steffan, S. and Scherer, S. 2003.A Pediocin-Producing Lactobacillus plantarum Strain Inhibits Listeria monocytogenes in a Multispecies Cheese Surface Microbial Ripening Consortium.Applied and Environmental Microbiology. 69:1854¿1857 Lopez, I., Ruiz-Larrea, F., Cocolin, L., Orr, E., Phister, T., Marshall, M., Vandergheynst, J., Mills, D.A., 2003.Design and evaluation of PCR Primers for Analysis of Bacterial Populations in Wine by Denaturing Gradient Gel Electrophoresis. Manachini, P.L.,and C. Parini. 1983. DNA restriction endonuclease cleavage patterns , DNA sequence similarity and phentotypical characteristics in some strains of Lactobacillus helveticus and Lactobacillus jugurti. Antoine van Leeuwenhoek 49:143 -152 Moreira, J.L., Mota,R., Horta, M., Teixeira, S,MR., Neumann,E., Nicoli, J., R., Nunes, A.,C. 2005. Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling. Nigatu, A. 2000. Evaluation of Numerical Analyses of RAPD and API 50 CH Patterns to Differentiate Lactobacillus plantarum,Lact. fermentum, Lact. sake, Lact. parabuchneri, Lact. gallinarum, Lact. casei, Weisella minor and related taxa Isolated from Kocho and Tef. Persing, D.H., Tenover, F.C., 2004.Molecular Microbiology, Diagnostic Principles and Practice, ASM Press, Washington D.C. Randazzo, C.L., Torriani, S., Akkermans, A.D.L., De Vos, W., Vaughan, E., 2002.Diversity, Dynamics, and Activity of Bacterial Communities during Production of an Artisanal Cheese as Evaluated by 16S rRNA Analysis. Salminen, S., Von Wright, A.,Ouwehand, A., 2004.Lactic Acid Bacteria, Microbiological and Functional Aspects, Third Edition, Marcel Dekker Inc. Swaminathan B., Matar, G.M., 1993.Molecular Typing Methods, Diagnostic Moleculas Microbiology, ASM Press, Washington D.C. Tannock, G.W., Tilsala-Timisjarvi, A, Rodtong, S. Ng, J., Munro, K.,Alatossava, T. 1999. Identification of Lactobacillus Isolates from the Gastrointestinal Tract,Silage, and Yoghurt by 16S- 23S rRNA Gene Intergenic Spacer Region Sequence Comparisons. Torriani, S., Zapparoli, G., Dellagio, F., 1999. Use of PCR-Based Methods for Rapid Differentiation of Lactobacillus delbrueckii sups. bulgaricus and L. delbrueckii subsp. lactis. Zhong, W., Millsap, K., Bialowska-Hobrzanska, H. Reid,G., 1998.Differentiation of Lactobacillus Species by Molecular Typing. Rivera, O., Maldonado, I. Universidad de Puerto Rico. Curso Biología General, Laboratorio 12. En: http://www.uprm.edu/biology/cursos/biologiageneral/EnzimasDNA.htm> [ Consulta: Septiembre 2006 ] http://industry.biomerieux-usa.com/industry/food/api/index.htm [ Consulta: Septiembre 2006 ] http://www.promega.com/catalog/country_select.asp?/enotes/applications/ap0063.htm&ckt=2 http://www.ncbi.nlm.nih.gov/ [ Consulta: Enero 2006 ] http://tools.neb.com/REBsites/index.php3 [ Consulta: Febrero 2006 ] http://www.idtdna.com/ [ Consulta: Enero 2006 ] https://hdl.handle.net/10818/4748 88366 TE03966
identifier_str_mv	Ausubel, F., Brent R., Kingston R., Seidman, J.G., 2005.Current Protocols in Molecular Biology, Volume 2, John Wiley & Sons. Cruz, J. G., 2006. Aislamiento y Caracterizacion Bioquimica de las Levaduras y de las Bacterias Acido lácticas Productoras de Sustancias Antimicrobianas de Interes Industrial Presentes en el ¿Suero Costeño¿. Tesis de Grado de Ingeniería de Producción Agroindustrial, Universidad de La Sabana. Jang,J., 2002. Development of 16S rDNA PCR-PFLP for Identification of Leuconostoc and Weisella species. Kim, B., Lee, J., Jang,J., Kim,J., Hang, H., 2003. Leuonostoc inhae sp. nov., a lactic acid bacterium isolated from kimchi. Lewin, B., 2001. Genes VII, Marbán, Madrid. Loessner, M., Guenther, S., Steffan, S. and Scherer, S. 2003.A Pediocin-Producing Lactobacillus plantarum Strain Inhibits Listeria monocytogenes in a Multispecies Cheese Surface Microbial Ripening Consortium.Applied and Environmental Microbiology. 69:1854¿1857 Lopez, I., Ruiz-Larrea, F., Cocolin, L., Orr, E., Phister, T., Marshall, M., Vandergheynst, J., Mills, D.A., 2003.Design and evaluation of PCR Primers for Analysis of Bacterial Populations in Wine by Denaturing Gradient Gel Electrophoresis. Manachini, P.L.,and C. Parini. 1983. DNA restriction endonuclease cleavage patterns , DNA sequence similarity and phentotypical characteristics in some strains of Lactobacillus helveticus and Lactobacillus jugurti. Antoine van Leeuwenhoek 49:143 -152 Moreira, J.L., Mota,R., Horta, M., Teixeira, S,MR., Neumann,E., Nicoli, J., R., Nunes, A.,C. 2005. Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling. Nigatu, A. 2000. Evaluation of Numerical Analyses of RAPD and API 50 CH Patterns to Differentiate Lactobacillus plantarum,Lact. fermentum, Lact. sake, Lact. parabuchneri, Lact. gallinarum, Lact. casei, Weisella minor and related taxa Isolated from Kocho and Tef. Persing, D.H., Tenover, F.C., 2004.Molecular Microbiology, Diagnostic Principles and Practice, ASM Press, Washington D.C. Randazzo, C.L., Torriani, S., Akkermans, A.D.L., De Vos, W., Vaughan, E., 2002.Diversity, Dynamics, and Activity of Bacterial Communities during Production of an Artisanal Cheese as Evaluated by 16S rRNA Analysis. Salminen, S., Von Wright, A.,Ouwehand, A., 2004.Lactic Acid Bacteria, Microbiological and Functional Aspects, Third Edition, Marcel Dekker Inc. Swaminathan B., Matar, G.M., 1993.Molecular Typing Methods, Diagnostic Moleculas Microbiology, ASM Press, Washington D.C. Tannock, G.W., Tilsala-Timisjarvi, A, Rodtong, S. Ng, J., Munro, K.,Alatossava, T. 1999. Identification of Lactobacillus Isolates from the Gastrointestinal Tract,Silage, and Yoghurt by 16S- 23S rRNA Gene Intergenic Spacer Region Sequence Comparisons. Torriani, S., Zapparoli, G., Dellagio, F., 1999. Use of PCR-Based Methods for Rapid Differentiation of Lactobacillus delbrueckii sups. bulgaricus and L. delbrueckii subsp. lactis. Zhong, W., Millsap, K., Bialowska-Hobrzanska, H. Reid,G., 1998.Differentiation of Lactobacillus Species by Molecular Typing. Rivera, O., Maldonado, I. Universidad de Puerto Rico. Curso Biología General, Laboratorio 12. En: http://www.uprm.edu/biology/cursos/biologiageneral/EnzimasDNA.htm> [ Consulta: Septiembre 2006 ] 88366 TE03966
url	http://industry.biomerieux-usa.com/industry/food/api/index.htm [ Consulta: Septiembre 2006 ] http://www.promega.com/catalog/country_select.asp?/enotes/applications/ap0063.htm&ckt=2 http://www.ncbi.nlm.nih.gov/ [ Consulta: Enero 2006 ] http://tools.neb.com/REBsites/index.php3 [ Consulta: Febrero 2006 ] http://www.idtdna.com/ [ Consulta: Enero 2006 ] https://hdl.handle.net/10818/4748
dc.language.iso.fl_str_mv	spa
language	spa
dc.rights.none.fl_str_mv	restrictedAccess
dc.rights.coar.fl_str_mv	http://purl.org/coar/access_right/c_16ec
rights_invalid_str_mv	restrictedAccess http://purl.org/coar/access_right/c_16ec
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Universidad de la Sabana Ingeniería de Producción Agroindustrial Facultad de ingeniería
publisher.none.fl_str_mv	Universidad de la Sabana Ingeniería de Producción Agroindustrial Facultad de ingeniería
dc.source.none.fl_str_mv	Intellectum Repositorio Universidad de la Sabana Universidad de la Sabana
institution	Universidad de la Sabana
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_	1860891720012529664
spelling	Caracterización molecular de bacterias ácido lácticas aisladas de suero costeñoBacteriasBacteriología del quesoBacteriología agrícolaEnzimas digestivas64 páginasEn la etapa previa de este proyecto se identificaron 31 de 40 aislamientos de bacterias ácido lácticas encontradas en muestras de suero costeño; dicha identificación se realizó usando la fermentación diferencial de azucares de cada bacteria. En este estudio se extrajo y purificó el ADN total de cada cepa a partir del cultivo fresco de cada uno de las cepas haciendo uso de un estuche comercial y se utilizaron unas cepas de control. Se estandarizó la técnica de PCR amplificando dos regiones de la subunidad 16S ribosomal y se realizó digestión enzimática a los fragmentos amplificados con dos enzimas de restricción. Los resultados confirmaron la identificación previa para algunas cepas, y mostraron posible variación genética de especie para otras.Universidad de la SabanaIngeniería de Producción AgroindustrialFacultad de ingenieríaCueto Vigil, María ClementinaSuárez Ramírez, Viviana2012-12-06T21:05:14Z2012-12-06T21:05:14Z20072007Tesis/Trabajo de grado - PregradopublishedVersionhttp://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85Textoinfo:eu-repo/semantics/bachelorThesishttp://purl.org/redcol/resource_type/TPapplication/pdfAusubel, F., Brent R., Kingston R., Seidman, J.G., 2005.Current Protocols in Molecular Biology, Volume 2, John Wiley & Sons.Cruz, J. G., 2006. Aislamiento y Caracterizacion Bioquimica de las Levaduras y de las Bacterias Acido lácticas Productoras de Sustancias Antimicrobianas de Interes Industrial Presentes en el ¿Suero Costeño¿. Tesis de Grado de Ingeniería de Producción Agroindustrial, Universidad de La Sabana.Jang,J., 2002. Development of 16S rDNA PCR-PFLP for Identification of Leuconostoc and Weisella species.Kim, B., Lee, J., Jang,J., Kim,J., Hang, H., 2003. Leuonostoc inhae sp. nov., a lactic acid bacterium isolated from kimchi.Lewin, B., 2001. Genes VII, Marbán, Madrid.Loessner, M., Guenther, S., Steffan, S. and Scherer, S. 2003.A Pediocin-Producing Lactobacillus plantarum Strain Inhibits Listeria monocytogenes in a Multispecies Cheese Surface Microbial Ripening Consortium.Applied and Environmental Microbiology. 69:1854¿1857Lopez, I., Ruiz-Larrea, F., Cocolin, L., Orr, E., Phister, T., Marshall, M., Vandergheynst, J., Mills, D.A., 2003.Design and evaluation of PCR Primers for Analysis of Bacterial Populations in Wine by Denaturing Gradient Gel Electrophoresis.Manachini, P.L.,and C. Parini. 1983. DNA restriction endonuclease cleavage patterns , DNA sequence similarity and phentotypical characteristics in some strains of Lactobacillus helveticus and Lactobacillus jugurti. Antoine van Leeuwenhoek 49:143 -152Moreira, J.L., Mota,R., Horta, M., Teixeira, S,MR., Neumann,E., Nicoli, J., R., Nunes, A.,C. 2005. Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling.Nigatu, A. 2000. Evaluation of Numerical Analyses of RAPD and API 50 CH Patterns to Differentiate Lactobacillus plantarum,Lact. fermentum, Lact. sake, Lact. parabuchneri, Lact. gallinarum, Lact. casei, Weisella minor and related taxa Isolated from Kocho and Tef.Persing, D.H., Tenover, F.C., 2004.Molecular Microbiology, Diagnostic Principles and Practice, ASM Press, Washington D.C.Randazzo, C.L., Torriani, S., Akkermans, A.D.L., De Vos, W., Vaughan, E., 2002.Diversity, Dynamics, and Activity of Bacterial Communities during Production of an Artisanal Cheese as Evaluated by 16S rRNA Analysis.Salminen, S., Von Wright, A.,Ouwehand, A., 2004.Lactic Acid Bacteria, Microbiological and Functional Aspects, Third Edition, Marcel Dekker Inc.Swaminathan B., Matar, G.M., 1993.Molecular Typing Methods, Diagnostic Moleculas Microbiology, ASM Press, Washington D.C.Tannock, G.W., Tilsala-Timisjarvi, A, Rodtong, S. Ng, J., Munro, K.,Alatossava, T. 1999. Identification of Lactobacillus Isolates from the Gastrointestinal Tract,Silage, and Yoghurt by 16S- 23S rRNA Gene Intergenic Spacer Region Sequence Comparisons.Torriani, S., Zapparoli, G., Dellagio, F., 1999. Use of PCR-Based Methods for Rapid Differentiation of Lactobacillus delbrueckii sups. bulgaricus and L. delbrueckii subsp. lactis.Zhong, W., Millsap, K., Bialowska-Hobrzanska, H. Reid,G., 1998.Differentiation of Lactobacillus Species by Molecular Typing.Rivera, O., Maldonado, I. Universidad de Puerto Rico. Curso Biología General, Laboratorio 12. En: http://www.uprm.edu/biology/cursos/biologiageneral/EnzimasDNA.htm> [ Consulta: Septiembre 2006 ]http://industry.biomerieux-usa.com/industry/food/api/index.htm [ Consulta: Septiembre 2006 ]http://www.promega.com/catalog/country_select.asp?/enotes/applications/ap0063.htm&ckt=2http://www.ncbi.nlm.nih.gov/ [ Consulta: Enero 2006 ]http://tools.neb.com/REBsites/index.php3 [ Consulta: Febrero 2006 ]http://www.idtdna.com/ [ Consulta: Enero 2006 ]https://hdl.handle.net/10818/474888366TE03966Intellectum Repositorio Universidad de la SabanaUniversidad de la SabanarestrictedAccesshttp://purl.org/coar/access_right/c_16ecspaoai:intellectum.unisabana.edu.co:10818/47482025-12-15T17:12:27Z

Caracterización molecular de bacterias ácido lácticas aisladas de suero costeño

Publicaciones similares