Evaluación de la obtención de bromelina por los métodos de extracción: bifases acuosas y salting out contenida en los corazones de las tres variedades de piña procesadas en la empresa Betters International S.A.S.
The objective of the present work was the evaluation of the Salting Out and Aqueous Biphases (ATPS) methods for the extraction of Bromelain contained in the pineapple core of three varieties, which are by-products of the pulping and peeling processes developed by the company BETTERS International SA...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2019
- Institución:
- Universidad de América
- Repositorio:
- Lumieres
- Idioma:
- spa
- OAI Identifier:
- oai:repository.uamerica.edu.co:20.500.11839/7368
- Acceso en línea:
- https://hdl.handle.net/20.500.11839/7368
- Palabra clave:
- Extraccion de enzimas
Salting Out
Sistema bifásico acuoso
Bromelina
Enzymes Extraction
Aqueous biphasic system
Bromelain
Tesis y disertaciones académicas
- Rights
- License
- Atribución – No comercial
| Summary: | The objective of the present work was the evaluation of the Salting Out and Aqueous Biphases (ATPS) methods for the extraction of Bromelain contained in the pineapple core of three varieties, which are by-products of the pulping and peeling processes developed by the company BETTERS International SAS. Each of the varieties was characterized by determining the amount of moisture, brix degrees, titratable acidity and pH before the Bromelain extraction process; during the entire experimental development process the storage of the raw material under refrigeration was required in order to slow down its deterioration. Following this, the two enzymatic extraction processes were implemented for each of the three worked varieties, which were: Amazonian, Mayanes and Golden MD2; an experimental design was made for each extraction method, with the purpose of statistically evaluating the interaction of the factors on the response variable. For the Salting Out process, the pH of the solution and percentage saturation of the mixture were selected as factors. The implementation of the system of aqueous biphases (ATPS) generated two phases in each of the experimental systems, the phase of interest was the TOP phase of each system where the largest amount of Bromelain was located in the system, the factor evaluated was the amount of dibasic potassium phosphate present in the solution. |
|---|