Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residuales

Figuras y tablas

Autores:
Barreto Montenegro, Julian Camilo
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2016
Institución:
Universidad de los Llanos
Repositorio:
Repositorio Digital Universidad de los LLanos
Idioma:
spa
OAI Identifier:
oai:repositorio.unillanos.edu.co:001/3024
Acceso en línea:
https://repositorio.unillanos.edu.co/handle/001/3024
https://repositorio.unillanos.edu.co/
Palabra clave:
Compuestos orgánicos
Ácidos orgánicos
Digestión anaerobia
Péptidos
Actividad antimicrobiana
Métodos de extracción
Anaerobic digestion
Peptides
Extraction methods
Antimicrobial activity
Rights
openAccess
License
Derechos Reservados - Universidad de los Llanos, 2016
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oai_identifier_str oai:repositorio.unillanos.edu.co:001/3024
network_acronym_str Unillanos2
network_name_str Repositorio Digital Universidad de los LLanos
repository_id_str
dc.title.spa.fl_str_mv Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residuales
title Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residuales
spellingShingle Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residuales
Compuestos orgánicos
Ácidos orgánicos
Digestión anaerobia
Péptidos
Actividad antimicrobiana
Métodos de extracción
Anaerobic digestion
Peptides
Extraction methods
Antimicrobial activity
title_short Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residuales
title_full Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residuales
title_fullStr Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residuales
title_full_unstemmed Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residuales
title_sort Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residuales
dc.creator.fl_str_mv Barreto Montenegro, Julian Camilo
dc.contributor.advisor.none.fl_str_mv Segura Latorre, Cesar
Pachón García, Jorge
dc.contributor.author.none.fl_str_mv Barreto Montenegro, Julian Camilo
dc.subject.armarc.none.fl_str_mv Compuestos orgánicos
Ácidos orgánicos
topic Compuestos orgánicos
Ácidos orgánicos
Digestión anaerobia
Péptidos
Actividad antimicrobiana
Métodos de extracción
Anaerobic digestion
Peptides
Extraction methods
Antimicrobial activity
dc.subject.proposal.spa.fl_str_mv Digestión anaerobia
Péptidos
Actividad antimicrobiana
Métodos de extracción
dc.subject.proposal.eng.fl_str_mv Anaerobic digestion
Peptides
Extraction methods
Antimicrobial activity
description Figuras y tablas
publishDate 2016
dc.date.issued.none.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2023-09-26T21:17:38Z
dc.date.available.none.fl_str_mv 2023-09-26T21:17:38Z
dc.type.spa.fl_str_mv Trabajo de grado - Pregrado
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/bachelorThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/TP
dc.type.coarversion.spa.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
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status_str publishedVersion
dc.identifier.citation.spa.fl_str_mv Barreto Montenegro, J. (2016). Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residuales [Trabajo de grado, Universidad de los Llanos]. Repositorio digital Universidad de los Llanos. https://repositorio.unillanos.edu.co/handle/001/3024
dc.identifier.uri.none.fl_str_mv https://repositorio.unillanos.edu.co/handle/001/3024
dc.identifier.instname.spa.fl_str_mv Universidad de los Llanos
dc.identifier.reponame.spa.fl_str_mv Repositorio digital Universidad de los Llanos
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unillanos.edu.co/
identifier_str_mv Barreto Montenegro, J. (2016). Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residuales [Trabajo de grado, Universidad de los Llanos]. Repositorio digital Universidad de los Llanos. https://repositorio.unillanos.edu.co/handle/001/3024
Universidad de los Llanos
Repositorio digital Universidad de los Llanos
url https://repositorio.unillanos.edu.co/handle/001/3024
https://repositorio.unillanos.edu.co/
dc.language.iso.spa.fl_str_mv spa
language spa
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dc.rights.spa.fl_str_mv Derechos Reservados - Universidad de los Llanos, 2016
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Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
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dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias Básicas e Ingeniería
dc.publisher.place.spa.fl_str_mv Villavicencio
institution Universidad de los Llanos
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spelling Segura Latorre, Cesar566b0b817d4cc430854c8d150ee2b947Pachón García, Jorgef44a9f1c70d24ddeb2ab3d05fdace26cBarreto Montenegro, Julian Camilo610598d64d2253e98584ff84c2a077852023-09-26T21:17:38Z2023-09-26T21:17:38Z2016Barreto Montenegro, J. (2016). Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residuales [Trabajo de grado, Universidad de los Llanos]. Repositorio digital Universidad de los Llanos. https://repositorio.unillanos.edu.co/handle/001/3024https://repositorio.unillanos.edu.co/handle/001/3024Universidad de los LlanosRepositorio digital Universidad de los Llanoshttps://repositorio.unillanos.edu.co/Figuras y tablasLa digestión anaerobia es un proceso fermentativo en ausencia de oxígeno llevado a cabo por comunidades microbianas. Se ha utilizado como una alternativa para el manejo de diversas fuentes residuales y para la producción de biogás. Entender las complejas interacciones y los procesos que involucran a estas comunidades microbianas es crítico para el mejoramiento de las condiciones anaerobias y buscar nuevas aplicaciones. Objetivo: Diseñar una metodología para la extracción y caracterización de péptidos derivados de la digestión anaerobia de lodos de la planta de tratamiento de aguas residuales de San Fernando (Itagüí, Antioquia). Para lograr este objetivo, el material peptídico se extrajo a partir de los lodos y se determinó la actividad antimicrobiana del extracto proteico. Metodología: Los péptidos se extrajeron mecánicamente por sonicación y se tamizaron a través de filtros moleculares de 10 KDa de peso molecular. La presencia y concentración de material proteico en los extractos (péptidos/proteínas pequeñas) se determinó por el método de Lowry. El extracto se caracterizó por cromatografía líquida de alta eficacia (HPLC), acoplado a espectrometría de masas de baja resolución (LC-MS) y electroforesis en gel (Tricina-SDS-PAGE).Anaerobic digestion is a fermentative proccess in the absence of oxygen. Carried out by microbial comunities, it has been an alternative for management of diverse sources of waste and biogas generation. Understanding the complex interactions and procesess involving microbial communities is critical for the improvement of anaerobic conditions and looking for new applications. Aim: Design a methodology for extraction and charaterization of peptides generated by anaerobic digestion of sludges from the Planta de Tratamiento de Aguas Residuales de San Fernando (Itagüí, Antioquía). To get this aim, peptidic material from sludge were extracted and the antimicrobial activity of the proteic extract was determined. Methodology: Peptides were mechanically extracted by sonication and filtered throughout 10 kDa molecular weight cutoff filters. Presence and concentration of proteic material in the extracts (peptides/small proteins) was determined by Lowry method, The extract was characterized by High Performance Liquid Chromatography (HPLC) online to low resolution mass spectrometry (LC-MS) and Tricine-SDSPAGE gel electrophoresis.Planteamiento del problema. -- Hipótesis. -- Objetivos. -- Objetivo general. -- Objetivos específicos. -- Justificación. -- Marco teórico. -- Digestión anaerobia. – Péptidos. – Metodología. -- Recolección de la muestra. -- Extracción de péptidos. -- Determinación de la concentración de proteína péptidos. -- Prueba de actividad antimicrobiana. -- Electroforesis Tricina-SDS-PAGE. -- Caracterización preliminar por HPLC. -- Cromatografía liquida acoplada a espectrometría de masa (LC-MS). -- Resultados. -- Determinación de la presencia de péptidos en el extracto. -- Prueba de actividad antimicrobiana. -- Electroforesis Tricina-SDS-PAGE. -- Caracterización preliminar por HPLC. -- Análisis por espectrometría de masas. -- Discusión. -- Determinación de la presencia/concentración de péptidos en el extracto. -- Prueba de actividad antimicrobiana. -- Electroforesis Tricina-SDS-PAGE. -- Caracterización preliminar por HPLC. -- Análisis por espectrometría de masas. – Conclusiones. -- Dificultades. -- Sugerencias. – Bibliografía. -- Anexos.PregradoBiólogoBiología136 páginasapplication/pdfspaUniversidad de los LlanosFacultad de Ciencias Básicas e IngenieríaVillavicencioDerechos Reservados - Universidad de los Llanos, 2016https://creativecommons.org/licenses/by-nc-nd/4.0/Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residualesTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttps://purl.org/redcol/resource_type/TPhttp://purl.org/coar/version/c_970fb48d4fbd8a85Abram, F., Enright, A. 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