Variación del metaboloma y la composición alcaloidal en Magnolia grandiflora (MAGNOLIACEAE) de la Sabana de Bogotá

En las interacciones de las plantas con su entorno se producen respuestas metabólicas a través de la síntesis de metabolitos secundarios y, por tanto, los factores ambientales y ecológicos que influyen en la planta establecen variaciones en sus perfiles metabólicos. En el caso de los alcaloides, que...

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Autores:: Ravagli Castillo, Andrea Carolina

Tipo de recurso:

Fecha de publicación:: 2017

Institución:: Universidad Militar Nueva Granada

Repositorio:: Repositorio UMNG

Idioma:: spa

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network_acronym_str	UNIMILTAR2
network_name_str	Repositorio UMNG
repository_id_str
dc.title.spa.fl_str_mv	Variación del metaboloma y la composición alcaloidal en Magnolia grandiflora (MAGNOLIACEAE) de la Sabana de Bogotá
dc.title.translated.spa.fl_str_mv	Variation of Metaboloma and Alkaloidal Composition in Magnolia grandiflora (MAGNOLIACEAE) of the Sabana of Bogotá
title	Variación del metaboloma y la composición alcaloidal en Magnolia grandiflora (MAGNOLIACEAE) de la Sabana de Bogotá
spellingShingle	Variación del metaboloma y la composición alcaloidal en Magnolia grandiflora (MAGNOLIACEAE) de la Sabana de Bogotá MAGNOLIACEAS ALCALOIDES Magnoliaceae Magnolia grandiflora Metabolic response Alkaloids Metaboloma Magnoliaceae Magnolia grandiflora Respuesta metabólica Alcaloides Metaboloma
title_short	Variación del metaboloma y la composición alcaloidal en Magnolia grandiflora (MAGNOLIACEAE) de la Sabana de Bogotá
title_full	Variación del metaboloma y la composición alcaloidal en Magnolia grandiflora (MAGNOLIACEAE) de la Sabana de Bogotá
title_fullStr	Variación del metaboloma y la composición alcaloidal en Magnolia grandiflora (MAGNOLIACEAE) de la Sabana de Bogotá
title_full_unstemmed	Variación del metaboloma y la composición alcaloidal en Magnolia grandiflora (MAGNOLIACEAE) de la Sabana de Bogotá
title_sort	Variación del metaboloma y la composición alcaloidal en Magnolia grandiflora (MAGNOLIACEAE) de la Sabana de Bogotá
dc.creator.fl_str_mv	Ravagli Castillo, Andrea Carolina
dc.contributor.advisor.spa.fl_str_mv	Coy Barrera, Ericsson
dc.contributor.author.spa.fl_str_mv	Ravagli Castillo, Andrea Carolina
dc.subject.lemb.spa.fl_str_mv	MAGNOLIACEAS ALCALOIDES
topic	MAGNOLIACEAS ALCALOIDES Magnoliaceae Magnolia grandiflora Metabolic response Alkaloids Metaboloma Magnoliaceae Magnolia grandiflora Respuesta metabólica Alcaloides Metaboloma
dc.subject.keywords.spa.fl_str_mv	Magnoliaceae Magnolia grandiflora Metabolic response Alkaloids Metaboloma
dc.subject.proposal.spa.fl_str_mv	Magnoliaceae Magnolia grandiflora Respuesta metabólica Alcaloides Metaboloma
description	En las interacciones de las plantas con su entorno se producen respuestas metabólicas a través de la síntesis de metabolitos secundarios y, por tanto, los factores ambientales y ecológicos que influyen en la planta establecen variaciones en sus perfiles metabólicos. En el caso de los alcaloides, que se encuentran ampliamente distribuidos en plantas y son agentes activos en las interacciones evolutivas debido a que su metabolismo está controlado bajo regulación genética, se convierten en componentes variables relacionados con acondicionamiento debido a presiones externas. En este contexto, como parte del interés del grupo en el estudio de respuestas metabólicas de angiospermas basales, se evaluó la variación del metaboloma y la composición alcaloidal de la especie Magnolia grandiflora (Magnoliaceae) distribuida en diferentes lugares. A partir de los extractos etanólicos de hojas y cortezas analizados por HPLC-DAD-MS se detectaron 23 compuestos secundarios, de los cuales 13 correspondían a alcaloides, acompañados de lignanos y neolignanos, lactonas sesquiterpénicas, antraquinona, y triterpeno. En el análisis por HPLC-DAD-MS de los extractos alcaloidales de hojas y cortezas, se detectaron 28 compuestos secundarios de los cuales 23 eran alcaloides entre los que se destacaron los tipos aporfinoide (dehidroaporfinas y dioxoaporfinas), isoquinolínicos y bis-bencilisoquinolínicos, morfinanos, y alcaloides derivados del tipo hasubanonina. El análisis multivariado supervisado (i.e., OPLS) con la cuantificación de fenoles conectó la naturaleza fenólica de ciertos alcaloides expresados por la planta que, en su mayoría, fueron del tipo aporfinoide, los cuales concuerdan con la incorporación de fenoles en su estructura por proceso biosintético. En general, el análisis multivariado confirmó por consiguiente que existe variación en los perfiles metabólicos y en la composición alcaloidal en relación a las partes de la planta evaluadas, los tiempos de colecta y los ambientes establecidos. Así mismo, varias muestras de hojas y cortezas biosintetizaron compuestos secundarios específicos, incluidos los alcaloides, en tiempos de colecta diferentes. Con los resultados obtenidos se puede establecer que la planta M. grandiflora presenta quimiotipos ricos en alcaloides, especialmente del tipo aporfinoide, en respuesta a condiciones ambientales y ecológicas diferentes.
publishDate	2017
dc.date.accessioned.none.fl_str_mv	2017-06-30T18:08:41Z 2019-12-26T21:08:55Z
dc.date.available.none.fl_str_mv	2017-06-30T18:08:41Z 2019-12-26T21:08:55Z
dc.date.issued.none.fl_str_mv	2017-04-21
dc.type.spa.fl_str_mv	info:eu-repo/semantics/bachelorThesis
dc.type.coar.fl_str_mv	http://purl.org/coar/resource_type/c_7a1f
dc.type.local.spa.fl_str_mv	Trabajo de grado
dc.identifier.uri.none.fl_str_mv	http://hdl.handle.net/10654/16163
url	http://hdl.handle.net/10654/16163
dc.language.iso.spa.fl_str_mv	spa
language	spa
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The alkaloids Chemistry and Biology. Volume 73. Elsevier, Oxford UK, pp. 178 Liscombe DK, MacLeod BP, Loukanina N, Nandi OI, Facchini PJ. 2005. Evidence for the monophyletic evolution of benzylisoquinoline alkaloid biosynthesis in angiosperms. Phytochemistry, 66(11): 1374-1393 Marcano D. y Hasegawa M. 2002. Fitoquímica orgánica. Segunda edición. Consejo de Desarrollo Científico y Humanístico. Universidad Central de Venezuela, pp. 384 Muzquiz M, Burbano C, Cuadrado C, De la Cuadra C. 1993. Determinación de factores antinutritivos termorresistentes en leguminosas I: Alcaloides. Inv. Agraria. Producción y Protección Veg., 8: 351-361 Phillipson JD, Roberts MF, Zenk MH. 1985. The chemistry and biology of Isoquinoline alkaloids. Springer-Verlag Berlin, Heidelberg, pp. 217-220 Thorne AD, Pexton JJ, Dytham C, Mayhew PJ. 2006. Small body size in an insect shifts development, prior to adult eclosion, towards early reproduction. Proc. R. Soc. B 273(1590): 1099-1103 Tzin V. y Galili G. 2010. 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Chemometrics 1 (1): 41-56 Zamora-Natera F, García-López P, Ruiz-López M, Salcedo-Pérez E. 2008. Composición de alcaloides en semillas de Lupinus mexicanus (fabaceae) y evaluación antifúngica y alelopática del extracto alcaloideo. Agrociencia, vol.42 no. 2 http://www.scielo.org.mx/scielo.php?pid=S1405-31952008000200006&script=sci_arttext
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spelling	Coy Barrera, EricssonRavagli Castillo, Andrea CarolinaBiólogoCampus UMNG2017-06-30T18:08:41Z2019-12-26T21:08:55Z2017-06-30T18:08:41Z2019-12-26T21:08:55Z2017-04-21http://hdl.handle.net/10654/16163En las interacciones de las plantas con su entorno se producen respuestas metabólicas a través de la síntesis de metabolitos secundarios y, por tanto, los factores ambientales y ecológicos que influyen en la planta establecen variaciones en sus perfiles metabólicos. En el caso de los alcaloides, que se encuentran ampliamente distribuidos en plantas y son agentes activos en las interacciones evolutivas debido a que su metabolismo está controlado bajo regulación genética, se convierten en componentes variables relacionados con acondicionamiento debido a presiones externas. En este contexto, como parte del interés del grupo en el estudio de respuestas metabólicas de angiospermas basales, se evaluó la variación del metaboloma y la composición alcaloidal de la especie Magnolia grandiflora (Magnoliaceae) distribuida en diferentes lugares. A partir de los extractos etanólicos de hojas y cortezas analizados por HPLC-DAD-MS se detectaron 23 compuestos secundarios, de los cuales 13 correspondían a alcaloides, acompañados de lignanos y neolignanos, lactonas sesquiterpénicas, antraquinona, y triterpeno. En el análisis por HPLC-DAD-MS de los extractos alcaloidales de hojas y cortezas, se detectaron 28 compuestos secundarios de los cuales 23 eran alcaloides entre los que se destacaron los tipos aporfinoide (dehidroaporfinas y dioxoaporfinas), isoquinolínicos y bis-bencilisoquinolínicos, morfinanos, y alcaloides derivados del tipo hasubanonina. El análisis multivariado supervisado (i.e., OPLS) con la cuantificación de fenoles conectó la naturaleza fenólica de ciertos alcaloides expresados por la planta que, en su mayoría, fueron del tipo aporfinoide, los cuales concuerdan con la incorporación de fenoles en su estructura por proceso biosintético. En general, el análisis multivariado confirmó por consiguiente que existe variación en los perfiles metabólicos y en la composición alcaloidal en relación a las partes de la planta evaluadas, los tiempos de colecta y los ambientes establecidos. Así mismo, varias muestras de hojas y cortezas biosintetizaron compuestos secundarios específicos, incluidos los alcaloides, en tiempos de colecta diferentes. Con los resultados obtenidos se puede establecer que la planta M. grandiflora presenta quimiotipos ricos en alcaloides, especialmente del tipo aporfinoide, en respuesta a condiciones ambientales y ecológicas diferentes.Vicerrectoría de Investigaciones de la Universidad Militar Nueva Granada. Proyecto IMP-CIAS-1567In the interactions of plants with their environment metabolic responses are produced through the synthesis of secondary metabolites and, therefore, the environmental and ecological factors that influence the plant establish variations in their metabolic profiles. In the case of alkaloids that are widely distributed in plants and are active agents in the evolutionary interactions because their metabolism is controlled under genetic regulation , they become variable components related to conditioning due to external pressures. In this context, as part of the group's interest in the study of metabolic responses of basal angiosperms, the variation of the metabolome and the alkaloidal composition of the species Magnolia grandiflora (Magnoliaceae) distributed in different places was evaluated. Twenty-three secondary compounds, of which 13 corresponded to alkaloids, were detected additionally lignans and neolignans, sesquiterpene lactones, anthraquinone, and triterpene from ethanolic leaf and bark extracts analyzed by HPLC-DAD-MS. In the HPLC-DAD-MS analysis of alkaloidal extracts of leaves and barks, 28 secondary compounds were detected, of which 23 were alkaloids, among which the aporphinoid (dehydrophosphine and dioxoaporfins), isoquinolinic and bis-benzylisoquinolinic, morphinan , and hasubanonine-derived alkaloids. Supervised multivariate analysis (ie, OPLS) with the quantification of phenols connected the phenolic nature of certain alkaloids expressed by the plant, which were mostly of the aporphinoid type, which are in agreement with the incorporation of phenols into its structure by biosynthetic process . In general, the multivariate analysis confirmed that there is indeed variation in the metabolic profiles and in the alkaloidal composition based on the parts of the plant evaluated, the collection times and the established environments. Also, several leaf and bark samples biosynthesized specific secondary compounds, including alkaloids, at different collection times. 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Agrociencia, vol.42 no. 2 http://www.scielo.org.mx/scielo.php?pid=S1405-31952008000200006&script=sci_arttexthttp://purl.org/coar/access_right/c_abf2ORIGINALRavagliCastilloAndreaCarolina2017.pdfTesisapplication/pdf3806006http://repository.unimilitar.edu.co/bitstream/10654/16163/1/RavagliCastilloAndreaCarolina2017.pdffd4063262ef3fe1803cdc4417fffa07cMD51LICENSElicense.txttext/plain1521http://repository.unimilitar.edu.co/bitstream/10654/16163/2/license.txt57c1b5429c07cf705f9d5e4ce515a2f6MD52TEXTRavagliCastilloAndreaCarolina2017.pdf.txtExtracted texttext/plain198658http://repository.unimilitar.edu.co/bitstream/10654/16163/3/RavagliCastilloAndreaCarolina2017.pdf.txta2815b71e9f1eb0f57c9aebfb495af0bMD53THUMBNAILRavagliCastilloAndreaCarolina2017.pdf.jpgIM Thumbnailimage/jpeg8763http://repository.unimilitar.edu.co/bitstream/10654/16163/4/RavagliCastilloAndreaCarolina2017.pdf.jpgb91addc91367e4ec1e4514795bc7b84aMD5410654/16163oai:repository.unimilitar.edu.co:10654/161632020-06-30 08:37:25.694Repositorio Institucional UMNGbibliodigital@unimilitar.edu.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