Synthesis and characterization of a novel colorimetric and fluorometric probe “Turn-on” for the detection of Cu2+ of derivatives rhodamine

A new Rhodamine-based “Turn On” fluorescent probe (E)- 3′,6′-bis(diethylamino)-2-((2,5-dimethoxybenzylidene)amino)spiro[isoindoline-1,9′-xanthen]-3-one (WGB) was synthesized. Results show that WGB is selective for Cu2+ cations, forming a WGB-Cu2+ complex in a 2:1 stoichiometry, confirmed through density functional theory (DFT) electronic structure calculations and reactive molecular dynamics (MD) simulations. Theoretical calculations agreed with the experimental data. The detection limit of WGB-Cu+2 complex is 6.76 × 10-8 M. Preliminary studies employing epifluorescence microscopy demonstrate that Cu2+ can be imaged in neuroblastoma SH-SY5Y cells treated with WGB.

• Autores: Zuzunaga Sanchez, Camilo Andres; Yañez, Osvaldo; Galdámez, Antonio; Tapia, Victoria; Núñez, Marco T; Osorio-Román, Igor; García, Camilo; García-Beltrán, Olimpo
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2023
• Institución: Universidad de Ibagué
• Repositorio: Repositorio Universidad de Ibagué
• Idioma: eng
• OAI Identifier: oai:repositorio.unibague.edu.co:20.500.12313/5581
• Acceso en línea: https://hdl.handle.net/20.500.12313/5581; https://www.sciencedirect.com/science/article/pii/S1010603022005019
• Palabra clave: Sonda colorimétrica - Características; Sonda fluorométrica - Características; Chemosensor; Colorimetric fluorescent dye; Cu2+ ions; Rhodamine derivate; Turn-On
• Rights: openAccess
• License: © 2022 The Author(s)