Amaryllidaceae alkaloids in skin cancer management: Photoprotective effect on human keratinocytes and anti-proliferative activity in melanoma cells

Skin cancer has high rates of mortality and therapeutic failure. In this study, to develop a multi-agent strategy for skin cancer management, the selective cytotoxicity of several alkaloid fractions and pure alkaloids isolated from Amaryllidaceae species was evaluated in melanoma cells. In addition, UVB-stimulated keratinocytes (HaCaT) were exposed to seven alkaloid fractions characterized by GC-MS, and the production of intracellular reactive oxygen species (ROS) and IL-6, were measured to evaluate their photoprotection effects. The Eucharis caucana (bulb) alkaloid fraction (20 μg/ml) had a clear effect on the viability of melanoma cells, reducing it by 45.7% without affecting healthy keratinocytes. This alkaloid fraction and tazettine (both at 2.5 μg/ml) suppressed UVB-induced ROS production by 31.6% and 29.4%, respectively. The highest anti-inflammatory potential was shown by the Zephyranthes carinata (bulb) alkaloid fraction (10 μg/ml), which reduced IL-6 production by 90.8%. According to the chemometric analysis, lycoramine and tazettine had a photoprotective effect on the UVB-exposed HaCaT cells, attenuating the production of ROS and IL-6. These results suggest that Amaryllidaceae alkaloids have photoprotective and therapeutic potential in skin cancer management, especially at low concentrations.

• Autores: Posada Correa, Gina Carolina; Bravo, Karent; Cortés, Natalie; Bedoya, Janeth; Borges, Warley de S.; Bastida, Jaume; Osorio Lopez, Edison Humberto
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2023
• Institución: Universidad de Ibagué
• Repositorio: Repositorio Universidad de Ibagué
• Idioma: eng
• OAI Identifier: oai:repositorio.unibague.edu.co:20.500.12313/5583
• Acceso en línea: https://hdl.handle.net/20.500.12313/5583; https://jab.zsf.jcu.cz/artkey/jab-202301-0005_amaryllidaceae-alkaloids-in-skin-cancer-management-photoprotective-effect-on-human-keratinocytes-and-anti-prol.php?back=%2Fsearch.php?query%3DAmaryllidaceae%2Balkaloids%2Bin%2Bskin%2Bcancer%2Bmanagement%253A%2BPhotoprotective%2Beffect%2Bon%2Bhuman%2Bkeratinocytes%2Band%2Banti-proliferative%2Bactivity%2Bin%2Bmelanoma%2Bcells%26sfrom%3D0%26spage%3D30
• Palabra clave: Cancer de piel; Alcaloides de Amaryllidaceae - Fotoprotector; Amaryllidaceae alkaloids; Eucharis caucana; Photoprotection; Skin cancer; Zephyranthes carinata
• Rights: openAccess
• License: © 2023 The Authors.