Conservando el gigante del Caribe: Ensamblaje denovo del Genoma del Mero Guasa (Epinephelus itajara) y su estructura poblacional, en el PNN Corales del Rosario y de San Bernardo
Epinephelus itajara, conocido como Mero guasa, es la especie más grande de la familia Epinephelidae y se encuentra en peligro crítico de extinción. Esta especie es de gran importancia ecológica, cultural y económica, y ha sido objeto de esfuerzos de reproducción en cautiverio en el Oceanario de las...
- Autores:
-
Jácome Castro, Juan Esteban
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2025
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/75891
- Acceso en línea:
- https://hdl.handle.net/1992/75891
- Palabra clave:
- Mero guasa
Epinephelus itajara
Cría en cautiverio
Genómica
Ensamble
Biología
- Rights
- openAccess
- License
- Attribution-ShareAlike 4.0 International
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Conservando el gigante del Caribe: Ensamblaje denovo del Genoma del Mero Guasa (Epinephelus itajara) y su estructura poblacional, en el PNN Corales del Rosario y de San Bernardo |
| title |
Conservando el gigante del Caribe: Ensamblaje denovo del Genoma del Mero Guasa (Epinephelus itajara) y su estructura poblacional, en el PNN Corales del Rosario y de San Bernardo |
| spellingShingle |
Conservando el gigante del Caribe: Ensamblaje denovo del Genoma del Mero Guasa (Epinephelus itajara) y su estructura poblacional, en el PNN Corales del Rosario y de San Bernardo Mero guasa Epinephelus itajara Cría en cautiverio Genómica Ensamble Biología |
| title_short |
Conservando el gigante del Caribe: Ensamblaje denovo del Genoma del Mero Guasa (Epinephelus itajara) y su estructura poblacional, en el PNN Corales del Rosario y de San Bernardo |
| title_full |
Conservando el gigante del Caribe: Ensamblaje denovo del Genoma del Mero Guasa (Epinephelus itajara) y su estructura poblacional, en el PNN Corales del Rosario y de San Bernardo |
| title_fullStr |
Conservando el gigante del Caribe: Ensamblaje denovo del Genoma del Mero Guasa (Epinephelus itajara) y su estructura poblacional, en el PNN Corales del Rosario y de San Bernardo |
| title_full_unstemmed |
Conservando el gigante del Caribe: Ensamblaje denovo del Genoma del Mero Guasa (Epinephelus itajara) y su estructura poblacional, en el PNN Corales del Rosario y de San Bernardo |
| title_sort |
Conservando el gigante del Caribe: Ensamblaje denovo del Genoma del Mero Guasa (Epinephelus itajara) y su estructura poblacional, en el PNN Corales del Rosario y de San Bernardo |
| dc.creator.fl_str_mv |
Jácome Castro, Juan Esteban |
| dc.contributor.advisor.none.fl_str_mv |
Caballero Gaitán, Susana Josefina Duitama Castellanos, Jorge Alexander Martínez, José Gregorio |
| dc.contributor.author.none.fl_str_mv |
Jácome Castro, Juan Esteban |
| dc.contributor.other.none.fl_str_mv |
Rojas Ruiz, Jaime Alberto Quiroga Camacho, Diego Alejandro Hakim de la Torre, Nadine |
| dc.contributor.jury.none.fl_str_mv |
Crawford, Andrew Jackson |
| dc.contributor.researchgroup.none.fl_str_mv |
Facultad de Ciencias::Laboratorio de Ecologia Molecular de Vertebrados Acuaticos |
| dc.subject.keyword.spa.fl_str_mv |
Mero guasa Epinephelus itajara Cría en cautiverio Genómica Ensamble |
| topic |
Mero guasa Epinephelus itajara Cría en cautiverio Genómica Ensamble Biología |
| dc.subject.themes.spa.fl_str_mv |
Biología |
| description |
Epinephelus itajara, conocido como Mero guasa, es la especie más grande de la familia Epinephelidae y se encuentra en peligro crítico de extinción. Esta especie es de gran importancia ecológica, cultural y económica, y ha sido objeto de esfuerzos de reproducción en cautiverio en el Oceanario de las Islas del Rosario, Colombia. Sin embargo, su estructura genética, adaptaciones evolutivas y dinámica poblacional son aún poco comprendidas en Colombia. Este estudio presenta un genoma de referencia con pseudocromosomas para E. itajara, obtenido mediante la tecnología de secuenciación PacBio HiFi. El genoma ensamblado tiene un tamaño aproximado de 1.1 Gb, con un contig N50 de 42.69 Mb y un scaffold N50 de 46.83 Mb. Fueron identificados 22,682 genes que codifican para proteínas después de enmascarar el 46% del genoma compuesto por elementos repetitivos. Además, se realizó un análisis de genómica poblacional utilizando 7,706 SNPs de alta calidad para evaluar la estructura genética de las poblaciones en cautiverio. Los resultados revelaron cuatro linajes genéticos diferenciados, con un flujo génico limitado entre ellos. En Colombia se identificaron dos linajes únicos, asociados a las localidades de Bahía Cispatá y Bahía Barbacoas, lo que sugiere un posible aislamiento geográfico. Estos recursos genómicos abren nuevas oportunidades y proporcionan herramientas valiosas para comprender la diversidad genética, la historia evolutiva y los mecanismos reproductivos de E. itajara, así como para diseñar estrategias de conservación, incluyendo programas de reproducción selectiva para aumentar la diversidad genética en cautiverio y guiar los esfuerzos de restauración en su hábitat natural. |
| publishDate |
2025 |
| dc.date.accessioned.none.fl_str_mv |
2025-01-31T13:34:56Z |
| dc.date.available.none.fl_str_mv |
2025-01-31T13:34:56Z |
| dc.date.issued.none.fl_str_mv |
2025-01-26 |
| dc.type.none.fl_str_mv |
Trabajo de grado - Pregrado |
| dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
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info:eu-repo/semantics/acceptedVersion |
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http://purl.org/coar/resource_type/c_7a1f |
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http://purl.org/redcol/resource_type/TP |
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https://hdl.handle.net/1992/75891 |
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instname:Universidad de los Andes |
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reponame:Repositorio Institucional Séneca |
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repourl:https://repositorio.uniandes.edu.co/ |
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instname:Universidad de los Andes reponame:Repositorio Institucional Séneca repourl:https://repositorio.uniandes.edu.co/ |
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spa |
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Harte Research Institute for Gulf of Mexico Studies, Special Publication Series, (1), 137-179. Sadovy, Y., & Eklund, A. M. (1999). Synopsis of biological data on the Nassau grouper, Epinephelus striatus (Bloch, 1792), and the jewfish, E. itajara (Lichtenstein, 1822). |
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Caballero Gaitán, Susana Josefinavirtual::22950-1Duitama Castellanos, Jorge Alexandervirtual::22951-1Martínez, José GregorioJácome Castro, Juan EstebanRojas Ruiz, Jaime AlbertoQuiroga Camacho, Diego AlejandroHakim de la Torre, NadineCrawford, Andrew JacksonFacultad de Ciencias::Laboratorio de Ecologia Molecular de Vertebrados Acuaticos2025-01-31T13:34:56Z2025-01-31T13:34:56Z2025-01-26https://hdl.handle.net/1992/75891instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Epinephelus itajara, conocido como Mero guasa, es la especie más grande de la familia Epinephelidae y se encuentra en peligro crítico de extinción. Esta especie es de gran importancia ecológica, cultural y económica, y ha sido objeto de esfuerzos de reproducción en cautiverio en el Oceanario de las Islas del Rosario, Colombia. Sin embargo, su estructura genética, adaptaciones evolutivas y dinámica poblacional son aún poco comprendidas en Colombia. Este estudio presenta un genoma de referencia con pseudocromosomas para E. itajara, obtenido mediante la tecnología de secuenciación PacBio HiFi. El genoma ensamblado tiene un tamaño aproximado de 1.1 Gb, con un contig N50 de 42.69 Mb y un scaffold N50 de 46.83 Mb. Fueron identificados 22,682 genes que codifican para proteínas después de enmascarar el 46% del genoma compuesto por elementos repetitivos. Además, se realizó un análisis de genómica poblacional utilizando 7,706 SNPs de alta calidad para evaluar la estructura genética de las poblaciones en cautiverio. Los resultados revelaron cuatro linajes genéticos diferenciados, con un flujo génico limitado entre ellos. En Colombia se identificaron dos linajes únicos, asociados a las localidades de Bahía Cispatá y Bahía Barbacoas, lo que sugiere un posible aislamiento geográfico. Estos recursos genómicos abren nuevas oportunidades y proporcionan herramientas valiosas para comprender la diversidad genética, la historia evolutiva y los mecanismos reproductivos de E. itajara, así como para diseñar estrategias de conservación, incluyendo programas de reproducción selectiva para aumentar la diversidad genética en cautiverio y guiar los esfuerzos de restauración en su hábitat natural.PADI FoundationPregrado30 páginasapplication/pdfspaUniversidad de los AndesBiologíaFacultad de CienciasDepartamento de Ciencias BiológicasAttribution-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Conservando el gigante del Caribe: Ensamblaje denovo del Genoma del Mero Guasa (Epinephelus itajara) y su estructura poblacional, en el PNN Corales del Rosario y de San BernardoTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPMero guasaEpinephelus itajaraCría en cautiverioGenómicaEnsambleBiologíaSouth Atlantic Fishery Management Council (SAFMC). Amendment Number 4, Regulatory Impact Review, Initial Regulatory Flexibility Analysis and Environmental Assessment for the Fishery Management Plan for the Snapper Grouper Fishery of the South Atlantic Region; South Atlantic Fishery Management Council: Charleston, SC, USA, 1990; p. 200Alonge, Michael, et al. "Automated assembly scaffolding elevates a new tomato system for high-throughput genome editing." Genome Biology (2022). https://doi.org/10.1186/s13059-022-02823-7Andrea Polanco F., Arturo Acero P. y Mónica Almanza. 2017. Epinephelus itajara. Páginas 61-64. En: Chasqui V., L., A. Polanco F., A. Acero P., P.A. Mejía-Falla, A. Navia, L.A. Zapata y J.P. Caldas. (Eds.). 2017. Libro rojo de peces marinos de Colombia. Instituto de Investigaciones Marinas y Costeras Invemar, Ministerio de Ambiente y Desarrollo Sostenible. Serie de Publicaciones Generales de INVEMAR # 93. Santa Marta, Colombia. 552 p.Birney, Ewan, Michele Clamp, and Richard Durbin. "GeneWise and genomewise." 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