Identification of an interaction between diguanylate cyclase CdgB and a putative interacting partner (PIP) in Streptomyces
Streptomyces is a group of filamentous bacteria, best known to produce diverse clinically important antibiotics. This group has a complex life cycle regulated by 3’-5’-cyclic diguanylic acid (c-diGMP). C-di-GMP is synthesized by enzymes called diguanylate cyclases (DGCs), and there are multiple gene...
- Autores:
-
Prada Mora, Juanita
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2025
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/75699
- Acceso en línea:
- https://hdl.handle.net/1992/75699
- Palabra clave:
- Streptomyces
3’-5’-cyclic diguanylic acid
Diguanylate cyclases
Bacterial Adenylate Cyclase Two-Hybrid system
Microbiología
- Rights
- openAccess
- License
- https://repositorio.uniandes.edu.co/static/pdf/aceptacion_uso_es.pdf
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Identification of an interaction between diguanylate cyclase CdgB and a putative interacting partner (PIP) in Streptomyces |
| title |
Identification of an interaction between diguanylate cyclase CdgB and a putative interacting partner (PIP) in Streptomyces |
| spellingShingle |
Identification of an interaction between diguanylate cyclase CdgB and a putative interacting partner (PIP) in Streptomyces Streptomyces 3’-5’-cyclic diguanylic acid Diguanylate cyclases Bacterial Adenylate Cyclase Two-Hybrid system Microbiología |
| title_short |
Identification of an interaction between diguanylate cyclase CdgB and a putative interacting partner (PIP) in Streptomyces |
| title_full |
Identification of an interaction between diguanylate cyclase CdgB and a putative interacting partner (PIP) in Streptomyces |
| title_fullStr |
Identification of an interaction between diguanylate cyclase CdgB and a putative interacting partner (PIP) in Streptomyces |
| title_full_unstemmed |
Identification of an interaction between diguanylate cyclase CdgB and a putative interacting partner (PIP) in Streptomyces |
| title_sort |
Identification of an interaction between diguanylate cyclase CdgB and a putative interacting partner (PIP) in Streptomyces |
| dc.creator.fl_str_mv |
Prada Mora, Juanita |
| dc.contributor.advisor.none.fl_str_mv |
Villegas Torres, Maria Francisca |
| dc.contributor.author.none.fl_str_mv |
Prada Mora, Juanita |
| dc.subject.keyword.none.fl_str_mv |
Streptomyces 3’-5’-cyclic diguanylic acid Diguanylate cyclases Bacterial Adenylate Cyclase Two-Hybrid system |
| topic |
Streptomyces 3’-5’-cyclic diguanylic acid Diguanylate cyclases Bacterial Adenylate Cyclase Two-Hybrid system Microbiología |
| dc.subject.themes.spa.fl_str_mv |
Microbiología |
| description |
Streptomyces is a group of filamentous bacteria, best known to produce diverse clinically important antibiotics. This group has a complex life cycle regulated by 3’-5’-cyclic diguanylic acid (c-diGMP). C-di-GMP is synthesized by enzymes called diguanylate cyclases (DGCs), and there are multiple genes that encode these enzymes. However, its function in regulating the life cycle is not completely clear. The deletion of the diguanylate cyclase-encoding gene, cdgB, affects the phenotype of the model species Streptomyces venezuelae, indicating its regulatory role in the organism's life cycle. In this internship, I was part of the Gallagher lab, and we aimed to implement the Bacterial Adenylate Cyclase two-hybrid system (BACTH) to determine possible candidates for protein-protein interaction with CdgB. The samples that showed positive interactions revealed alignment with various genes. A putative interacting partner (PIP) was screened directly through BACTH system, but the initial results were inconclusive. |
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2025 |
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2025-01-27T21:07:37Z |
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2025-01-27T21:07:37Z |
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2025-01-23 |
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Trabajo de grado - Pregrado |
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info:eu-repo/semantics/bachelorThesis |
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info:eu-repo/semantics/acceptedVersion |
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http://purl.org/coar/resource_type/c_7a1f |
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https://hdl.handle.net/1992/75699 |
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instname:Universidad de los Andes |
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eng |
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eng |
| dc.relation.references.none.fl_str_mv |
Schlimpert, S., & Elliot, M. A. (2023). The Best of Both Worlds-Streptomyces coelicolor and Streptomyces venezuelae as Model Species for Studying Antibiotic Production and Bacterial Multicellular Development. Journal of bacteriology, 205(7), e0015323. https://doi.org/10.1128/jb.00153-23 Gallagher, K. A., Schumacher, M. A., Bush, M. J., Bibb, M. J., Chandra, G., Holmes, N. A., Zeng, W., Henderson, M., Zhang, H., Findlay, K. C., Brennan, R. G., & Buttner, M. J. (2020). c-di-GMP Arms an Anti-σ to Control Progression of Multicellular Differentiation in Streptomyces. Molecular cell, 77(3), 586–599.e6. https://doi.org/10.1016/j.molcel.2019.11.006 Al-Bassam, M. M., Haist, J., Neumann, S. A., Lindenberg, S., & Tschowri, N. (2018). Expression Patterns, Genomic Conservation and Input Into Developmental Regulation of the GGDEF/EAL/HD-GYP Domain Proteins in Streptomyces. Frontiers in microbiology, 9, 2524. https://doi.org/10.3389/fmicb.2018.02524 Jenal, U., Reinders, A., & Lori, C. (2017). Cyclic di-GMP: second messenger extraordinaire. Nature reviews. Microbiology, 15(5), 271–284. https://doi.org/10.1038/nrmicro.2016.190 Schumacher, M. A., Gallagher, K. A., Holmes, N. A., Chandra, G., Henderson, M., Kysela, D. T., Brennan, R. G., & Buttner, M. J. (2021). Evolution of a σ-(c-di-GMP)-antiσ switch. Proceedings of the National Academy of Sciences of the United States of America, 118(30), e2105447118. https://doi.org/10.1073/pnas.2105447118 Haist, J., Neumann, S. A., Al-Bassam, M. M., Lindenberg, S., Elliot, M. A., & Tschowri, N. (2020). Specialized and shared functions of diguanylate cyclases and phosphodiesterases in Streptomyces development. Molecular microbiology, 114(5), 808–822. https://doi.org/10.1111/mmi.14581 Olson, M. G., Goldammer, M., Gauliard, E., Ladant, D., & Ouellette, S. P. (2018). A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning. Methods in molecular biology (Clifton, N.J.), 1794, 75–96. https://doi.org/10.1007/978-1-4939-7871-7_6 |
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Universidad de los Andes |
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Villegas Torres, Maria Franciscavirtual::22654-1Prada Mora, Juanita2025-01-27T21:07:37Z2025-01-27T21:07:37Z2025-01-23https://hdl.handle.net/1992/75699instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Streptomyces is a group of filamentous bacteria, best known to produce diverse clinically important antibiotics. This group has a complex life cycle regulated by 3’-5’-cyclic diguanylic acid (c-diGMP). C-di-GMP is synthesized by enzymes called diguanylate cyclases (DGCs), and there are multiple genes that encode these enzymes. However, its function in regulating the life cycle is not completely clear. The deletion of the diguanylate cyclase-encoding gene, cdgB, affects the phenotype of the model species Streptomyces venezuelae, indicating its regulatory role in the organism's life cycle. In this internship, I was part of the Gallagher lab, and we aimed to implement the Bacterial Adenylate Cyclase two-hybrid system (BACTH) to determine possible candidates for protein-protein interaction with CdgB. The samples that showed positive interactions revealed alignment with various genes. A putative interacting partner (PIP) was screened directly through BACTH system, but the initial results were inconclusive.Pregrado13 páginasapplication/pdfengUniversidad de los AndesMicrobiologíaFacultad de CienciasDepartamento de Ciencias Biológicashttps://repositorio.uniandes.edu.co/static/pdf/aceptacion_uso_es.pdfinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Identification of an interaction between diguanylate cyclase CdgB and a putative interacting partner (PIP) in StreptomycesTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPStreptomyces3’-5’-cyclic diguanylic acidDiguanylate cyclasesBacterial Adenylate Cyclase Two-Hybrid systemMicrobiologíaSchlimpert, S., & Elliot, M. A. (2023). The Best of Both Worlds-Streptomyces coelicolor and Streptomyces venezuelae as Model Species for Studying Antibiotic Production and Bacterial Multicellular Development. Journal of bacteriology, 205(7), e0015323.https://doi.org/10.1128/jb.00153-23Gallagher, K. A., Schumacher, M. A., Bush, M. J., Bibb, M. J., Chandra, G., Holmes, N. A., Zeng, W., Henderson, M., Zhang, H., Findlay, K. C., Brennan, R. G., & Buttner, M. J. (2020). c-di-GMP Arms an Anti-σ to Control Progression of Multicellular Differentiationin Streptomyces. Molecular cell, 77(3), 586–599.e6. https://doi.org/10.1016/j.molcel.2019.11.006Al-Bassam, M. M., Haist, J., Neumann, S. A., Lindenberg, S., & Tschowri, N. (2018). Expression Patterns, Genomic Conservation and Input Into Developmental Regulation of the GGDEF/EAL/HD-GYP Domain Proteins in Streptomyces. Frontiers in microbiology, 9, 2524. https://doi.org/10.3389/fmicb.2018.02524Jenal, U., Reinders, A., & Lori, C. (2017). Cyclic di-GMP: second messenger extraordinaire. Nature reviews. Microbiology, 15(5), 271–284. https://doi.org/10.1038/nrmicro.2016.190Schumacher, M. A., Gallagher, K. A., Holmes, N. A., Chandra, G., Henderson, M., Kysela, D. T., Brennan, R. G., & Buttner, M. J. (2021). Evolution of a σ-(c-di-GMP)-antiσ switch. Proceedings of the National Academy of Sciences of the United States of America, 118(30), e2105447118. https://doi.org/10.1073/pnas.2105447118Haist, J., Neumann, S. A., Al-Bassam, M. M., Lindenberg, S., Elliot, M. A., & Tschowri, N. (2020). Specialized and shared functions of diguanylate cyclases and phosphodiesterases in Streptomyces development. Molecular microbiology, 114(5), 808–822. https://doi.org/10.1111/mmi.14581Olson, M. G., Goldammer, M., Gauliard, E., Ladant, D., & Ouellette, S. P. (2018). A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning. Methods in molecular biology (Clifton, N.J.), 1794, 75–96. https://doi.org/10.1007/978-1-4939-7871-7_6202013169Publicationhttps://scholar.google.es/citations?user=OY35UmAAAAAJvirtual::22654-10000-0001-7067-4113virtual::22654-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000892203virtual::22654-15cfe6ba7-9a70-4327-b4fb-45d641e1396bvirtual::22654-15cfe6ba7-9a70-4327-b4fb-45d641e1396bvirtual::22654-1ORIGINALIdentification of an interaction between diguanylate cyclase CdgB and a putative interacting partner (PIP) in Streptomyces.pdfIdentification of an interaction between diguanylate cyclase CdgB and a putative interacting partner (PIP) in 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