The physical properties of the cytoplasm change during aging in s. cerevisiae

Age-related diseases are among the leading causes of mortality, with conditions like cardiovascular diseases, cancer, and diabetes escalating exponentially with age. Delaying aging may have a bigger payoff than tackling individual diseases.To achieve this goal, a deeper understanding of aging at the...

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Autores:
Durán Chaparro, David Camilo
Tipo de recurso:
Doctoral thesis
Fecha de publicación:
2025
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/75865
Acceso en línea:
https://hdl.handle.net/1992/75865
Palabra clave:
Aging
Macromolecular crowding
Microfluidics
Yeast
Diffusion
Saccharomyces cerevisiae
Slipstreaming
Replicative Aging
Chronological Aging
Genetically Encoded Multimeric Nanoparticles (GEMs)
Physical Properties of the Cell
Physics of Aging
Física
Biología
Ingeniería
Rights
openAccess
License
Attribution-NonCommercial 4.0 International
id UNIANDES2_449b618867d4012960e13affbbcd666f
oai_identifier_str oai:repositorio.uniandes.edu.co:1992/75865
network_acronym_str UNIANDES2
network_name_str Séneca: repositorio Uniandes
repository_id_str
dc.title.eng.fl_str_mv The physical properties of the cytoplasm change during aging in s. cerevisiae
title The physical properties of the cytoplasm change during aging in s. cerevisiae
spellingShingle The physical properties of the cytoplasm change during aging in s. cerevisiae
Aging
Macromolecular crowding
Microfluidics
Yeast
Diffusion
Saccharomyces cerevisiae
Slipstreaming
Replicative Aging
Chronological Aging
Genetically Encoded Multimeric Nanoparticles (GEMs)
Physical Properties of the Cell
Physics of Aging
Física
Biología
Ingeniería
title_short The physical properties of the cytoplasm change during aging in s. cerevisiae
title_full The physical properties of the cytoplasm change during aging in s. cerevisiae
title_fullStr The physical properties of the cytoplasm change during aging in s. cerevisiae
title_full_unstemmed The physical properties of the cytoplasm change during aging in s. cerevisiae
title_sort The physical properties of the cytoplasm change during aging in s. cerevisiae
dc.creator.fl_str_mv Durán Chaparro, David Camilo
dc.contributor.advisor.none.fl_str_mv Pedraza Leal, Juan Manuel
dc.contributor.author.none.fl_str_mv Durán Chaparro, David Camilo
dc.contributor.jury.none.fl_str_mv Leidy, Chad
Veenhoff, Liesbeth M.
Holt, Liam J.
dc.contributor.researchgroup.none.fl_str_mv Facultad de Ciencias
dc.subject.keyword.eng.fl_str_mv Aging
Macromolecular crowding
Microfluidics
Yeast
Diffusion
Saccharomyces cerevisiae
Slipstreaming
Replicative Aging
Chronological Aging
Genetically Encoded Multimeric Nanoparticles (GEMs)
Physical Properties of the Cell
Physics of Aging
topic Aging
Macromolecular crowding
Microfluidics
Yeast
Diffusion
Saccharomyces cerevisiae
Slipstreaming
Replicative Aging
Chronological Aging
Genetically Encoded Multimeric Nanoparticles (GEMs)
Physical Properties of the Cell
Physics of Aging
Física
Biología
Ingeniería
dc.subject.themes.none.fl_str_mv Física
Biología
Ingeniería
description Age-related diseases are among the leading causes of mortality, with conditions like cardiovascular diseases, cancer, and diabetes escalating exponentially with age. Delaying aging may have a bigger payoff than tackling individual diseases.To achieve this goal, a deeper understanding of aging at the cellular level is crucial. While several hallmarks of aging have been identified, there is still an absence of a Unified Theory of Aging. Many of these hallmarks appear to be interconnected through physical phenomena like phase separation. Physical Properties hold promise as a unifying framework, potentially offering avenues for rejuvenating cells through physical interventions. Macromolecular crowding emerges as a key aspect in this context, intimately linked with phase separation. While its effects have been observed at nanometer and organelle scales, understanding crowding at the mesoscale (10-100 nm) remains elusive. Our study delves into this mesoscale crowding using Genetically Encoded Multimeric Nanoparticles (GEMs), revealing how changes in cytoplasmic physical properties correlate with different cellular states during the aging process. Our research sheds light on the intricate relationship between macromolecular crowding, phase separation, and cellular aging, offering insights that could pave the way for novel therapeutic interventions. Additionally, we highlight potential biases in traditional single-cell aging studies due to cell trapping pressures. To address this, we developed a microfluidic device that makes use of the Slipstreaming Effect to trap single cells in a low-pressure environment, providing a more accurate representation of cellular aging. This study aims to contribute valuable insights into the aging process, ultimately striving to enhance the quality of life for individuals.
publishDate 2025
dc.date.accessioned.none.fl_str_mv 2025-01-30T19:53:52Z
dc.date.available.none.fl_str_mv 2025-01-30T19:53:52Z
dc.date.issued.none.fl_str_mv 2025-01-29
dc.type.none.fl_str_mv Trabajo de grado - Doctorado
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
dc.type.version.none.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_db06
dc.type.content.none.fl_str_mv Text
dc.type.redcol.none.fl_str_mv https://purl.org/redcol/resource_type/TD
format http://purl.org/coar/resource_type/c_db06
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/1992/75865
dc.identifier.instname.none.fl_str_mv instname:Universidad de los Andes
dc.identifier.reponame.none.fl_str_mv reponame:Repositorio Institucional Séneca
dc.identifier.repourl.none.fl_str_mv repourl:https://repositorio.uniandes.edu.co/
url https://hdl.handle.net/1992/75865
identifier_str_mv instname:Universidad de los Andes
reponame:Repositorio Institucional Séneca
repourl:https://repositorio.uniandes.edu.co/
dc.language.iso.none.fl_str_mv eng
language eng
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spelling Pedraza Leal, Juan Manuelvirtual::22891-1Durán Chaparro, David CamiloLeidy, ChadVeenhoff, Liesbeth M.Holt, Liam J.Facultad de Ciencias2025-01-30T19:53:52Z2025-01-30T19:53:52Z2025-01-29https://hdl.handle.net/1992/75865instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Age-related diseases are among the leading causes of mortality, with conditions like cardiovascular diseases, cancer, and diabetes escalating exponentially with age. Delaying aging may have a bigger payoff than tackling individual diseases.To achieve this goal, a deeper understanding of aging at the cellular level is crucial. While several hallmarks of aging have been identified, there is still an absence of a Unified Theory of Aging. Many of these hallmarks appear to be interconnected through physical phenomena like phase separation. Physical Properties hold promise as a unifying framework, potentially offering avenues for rejuvenating cells through physical interventions. Macromolecular crowding emerges as a key aspect in this context, intimately linked with phase separation. While its effects have been observed at nanometer and organelle scales, understanding crowding at the mesoscale (10-100 nm) remains elusive. Our study delves into this mesoscale crowding using Genetically Encoded Multimeric Nanoparticles (GEMs), revealing how changes in cytoplasmic physical properties correlate with different cellular states during the aging process. Our research sheds light on the intricate relationship between macromolecular crowding, phase separation, and cellular aging, offering insights that could pave the way for novel therapeutic interventions. Additionally, we highlight potential biases in traditional single-cell aging studies due to cell trapping pressures. To address this, we developed a microfluidic device that makes use of the Slipstreaming Effect to trap single cells in a low-pressure environment, providing a more accurate representation of cellular aging. This study aims to contribute valuable insights into the aging process, ultimately striving to enhance the quality of life for individuals.Universidad de los AndesNYU Langone HealthDoctorado228 páginasapplication/pdfengUniversidad de los AndesDoctorado en Ciencias - FísicaFacultad de CienciasDepartamento de FísicaAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2The physical properties of the cytoplasm change during aging in s. cerevisiaeTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttps://purl.org/redcol/resource_type/TDAgingMacromolecular crowdingMicrofluidicsYeastDiffusionSaccharomyces cerevisiaeSlipstreamingReplicative AgingChronological AgingGenetically Encoded Multimeric Nanoparticles (GEMs)Physical Properties of the CellPhysics of AgingFísicaBiologíaIngeniería[1] B. 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