The physical properties of the cytoplasm change during aging in s. cerevisiae

Age-related diseases are among the leading causes of mortality, with conditions like cardiovascular diseases, cancer, and diabetes escalating exponentially with age. Delaying aging may have a bigger payoff than tackling individual diseases.To achieve this goal, a deeper understanding of aging at the...

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Autores:: Durán Chaparro, David Camilo

Tipo de recurso:: Doctoral thesis

Fecha de publicación:: 2025

Institución:: Universidad de los Andes

Repositorio:: Séneca: repositorio Uniandes

Idioma:: eng

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Summary:	Age-related diseases are among the leading causes of mortality, with conditions like cardiovascular diseases, cancer, and diabetes escalating exponentially with age. Delaying aging may have a bigger payoff than tackling individual diseases.To achieve this goal, a deeper understanding of aging at the cellular level is crucial. While several hallmarks of aging have been identified, there is still an absence of a Unified Theory of Aging. Many of these hallmarks appear to be interconnected through physical phenomena like phase separation. Physical Properties hold promise as a unifying framework, potentially offering avenues for rejuvenating cells through physical interventions. Macromolecular crowding emerges as a key aspect in this context, intimately linked with phase separation. While its effects have been observed at nanometer and organelle scales, understanding crowding at the mesoscale (10-100 nm) remains elusive. Our study delves into this mesoscale crowding using Genetically Encoded Multimeric Nanoparticles (GEMs), revealing how changes in cytoplasmic physical properties correlate with different cellular states during the aging process. Our research sheds light on the intricate relationship between macromolecular crowding, phase separation, and cellular aging, offering insights that could pave the way for novel therapeutic interventions. Additionally, we highlight potential biases in traditional single-cell aging studies due to cell trapping pressures. To address this, we developed a microfluidic device that makes use of the Slipstreaming Effect to trap single cells in a low-pressure environment, providing a more accurate representation of cellular aging. This study aims to contribute valuable insights into the aging process, ultimately striving to enhance the quality of life for individuals.

The physical properties of the cytoplasm change during aging in s. cerevisiae

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