Challenge in the diagnosis of Staphylococcus aureus: Discordance between the genotypical and phenotypical profiles

Staphylococcus aureus is a superbug because of its molecular and phenotypical features. That makes this pathogen challenging to track and to establish a successful antibiotic treatment. In this research, 57 S. aureus clinical isolates were characterized by their molecular and phenotypical properties...

Full description

Autores:
Mancera García, María Alejandra
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2021
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
spa
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/55071
Acceso en línea:
http://hdl.handle.net/1992/55071
Palabra clave:
Aislamientos clínicos
Staphylococcus aureus
Rastreo epidemiológico
SARM
Aislamientos de comunidad
Microbiología
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/4.0/
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oai_identifier_str oai:repositorio.uniandes.edu.co:1992/55071
network_acronym_str UNIANDES2
network_name_str Séneca: repositorio Uniandes
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dc.title.eng.fl_str_mv Challenge in the diagnosis of Staphylococcus aureus: Discordance between the genotypical and phenotypical profiles
title Challenge in the diagnosis of Staphylococcus aureus: Discordance between the genotypical and phenotypical profiles
spellingShingle Challenge in the diagnosis of Staphylococcus aureus: Discordance between the genotypical and phenotypical profiles
Aislamientos clínicos
Staphylococcus aureus
Rastreo epidemiológico
SARM
Aislamientos de comunidad
Microbiología
title_short Challenge in the diagnosis of Staphylococcus aureus: Discordance between the genotypical and phenotypical profiles
title_full Challenge in the diagnosis of Staphylococcus aureus: Discordance between the genotypical and phenotypical profiles
title_fullStr Challenge in the diagnosis of Staphylococcus aureus: Discordance between the genotypical and phenotypical profiles
title_full_unstemmed Challenge in the diagnosis of Staphylococcus aureus: Discordance between the genotypical and phenotypical profiles
title_sort Challenge in the diagnosis of Staphylococcus aureus: Discordance between the genotypical and phenotypical profiles
dc.creator.fl_str_mv Mancera García, María Alejandra
dc.contributor.advisor.none.fl_str_mv Celis Ramírez, Adriana Marcela
dc.contributor.author.spa.fl_str_mv Mancera García, María Alejandra
dc.subject.keyword.none.fl_str_mv Aislamientos clínicos
Staphylococcus aureus
Rastreo epidemiológico
SARM
Aislamientos de comunidad
topic Aislamientos clínicos
Staphylococcus aureus
Rastreo epidemiológico
SARM
Aislamientos de comunidad
Microbiología
dc.subject.themes.none.fl_str_mv Microbiología
description Staphylococcus aureus is a superbug because of its molecular and phenotypical features. That makes this pathogen challenging to track and to establish a successful antibiotic treatment. In this research, 57 S. aureus clinical isolates were characterized by their molecular and phenotypical properties: The detection of the mecA resistance gene, the pvl virulence gene, Spa typing, and their antibiotic susceptibility profile. S. aureus isolates were classified as methicillin-resistant S. aureus (MRSA), methicillin-sensible S. aureus (MSSA), oxacillin sensible methicillin-resistant S. aureus (OS-MRSA), and borderline oxacillin-resistant S. aureus (BORSA). MRSA correspond to 47% of the total isolates. The presumptive origin of MRSA infection was mainly related to community environments (57% CA-MRSA). We would conclude that community-acquired (CA-MRSA) isolates are replacing healthcare-associated (HA-MRSA). The presence of pvl was detected in almost every clinical group, (e.g., multi-resistant MSSA isolates), and determine this as an ambiguous marker for CA-MRSA. Finally, the spa-typing results allowed us to identify the possible establishment of pandemic clones in Bogotá-Colombia. The characterization of Staphylococcus aureus clinical types is difficult because of transferable mobile genetic elements that harbor remarkable resistance and virulent genes; nonetheless, their accurate identification is necessary to guarantee therapeutic success.
publishDate 2021
dc.date.issued.none.fl_str_mv 2021
dc.date.accessioned.none.fl_str_mv 2022-02-22T19:49:49Z
dc.date.available.none.fl_str_mv 2022-02-22T19:49:49Z
dc.type.spa.fl_str_mv Trabajo de grado - Pregrado
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dc.identifier.pdf.spa.fl_str_mv 26444.pdf
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dc.format.extent.spa.fl_str_mv 19 páginas
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dc.publisher.spa.fl_str_mv Universidad de los Andes
dc.publisher.program.spa.fl_str_mv Microbiología
dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias
dc.publisher.department.spa.fl_str_mv Departamento de Ciencias Biológicas
institution Universidad de los Andes
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spelling Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Celis Ramírez, Adriana Marcelavirtual::17495-1Mancera García, María Alejandraeae1dca1-9f6f-42f4-9ebb-cc47fde552285002022-02-22T19:49:49Z2022-02-22T19:49:49Z2021http://hdl.handle.net/1992/5507126444.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Staphylococcus aureus is a superbug because of its molecular and phenotypical features. That makes this pathogen challenging to track and to establish a successful antibiotic treatment. In this research, 57 S. aureus clinical isolates were characterized by their molecular and phenotypical properties: The detection of the mecA resistance gene, the pvl virulence gene, Spa typing, and their antibiotic susceptibility profile. S. aureus isolates were classified as methicillin-resistant S. aureus (MRSA), methicillin-sensible S. aureus (MSSA), oxacillin sensible methicillin-resistant S. aureus (OS-MRSA), and borderline oxacillin-resistant S. aureus (BORSA). MRSA correspond to 47% of the total isolates. The presumptive origin of MRSA infection was mainly related to community environments (57% CA-MRSA). We would conclude that community-acquired (CA-MRSA) isolates are replacing healthcare-associated (HA-MRSA). The presence of pvl was detected in almost every clinical group, (e.g., multi-resistant MSSA isolates), and determine this as an ambiguous marker for CA-MRSA. Finally, the spa-typing results allowed us to identify the possible establishment of pandemic clones in Bogotá-Colombia. The characterization of Staphylococcus aureus clinical types is difficult because of transferable mobile genetic elements that harbor remarkable resistance and virulent genes; nonetheless, their accurate identification is necessary to guarantee therapeutic success.Staphylococcus aureus es un poderoso patógeno humano debido a sus característica moleculares y fenotípicas que lo hacen un microorganismo de difícil rastreo y tratamiento antibiótico. En esta investigación se caracterizaron 57 aislamientos clínicos S. aureus mediante su perfil antibiótico y molecular. La detección del gen de resistencia mecA, el gen de virulencia pvl y rastreo epidemiológico mediante spa-typing fue realizada. Los aislamientos fueron clasificados como S. aureus resistente a meticilina (SARM), S. aureus sensible a meticilina (SASM), S. aureus sensible a oxacilina y resistente a meticilina (SARM-OS), y S. aureus con resistencia limítrofe a oxacilina (BORSA). El grupo clínico con mayor proporción correspondió a SARM (47% de los aislamientos) concordando con reportes previos. El origen de los aislamientos fue clasificado como infección adquirida en comunidad (SARM -AC) u hospital (SARM-AH), donde el 52.6% de SARM fue presuntivo de origen en comunidad. Por lo anterior, se puede concluir que los aislamientos de comunidad están reemplazando a los de hospital. El gen pvl fue detectado en todos los grupos clínicos, por lo que no puede definirse como un factor de clasificación de SARM- AC. Asimismo, la tipificación molecular del gen spa nos permitió identificar cuáles fueron los clones pandémicos exitosos en Bogotá- Colombia. Finalmente, pudimos concluir que la identificación y clasificación de cepas S. aureus es desafiante debido a la transferencia de elementos móviles que portan genes significativos para la resistencia y virulencia, de identificación obligatoria para el éxito terapéutico.MicrobiólogoPregrado19 páginasapplication/pdfspaUniversidad de los AndesMicrobiologíaFacultad de CienciasDepartamento de Ciencias BiológicasChallenge in the diagnosis of Staphylococcus aureus: Discordance between the genotypical and phenotypical profilesTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPAislamientos clínicosStaphylococcus aureusRastreo epidemiológicoSARMAislamientos de comunidadMicrobiología201425114Publication0000-0003-3057-1966virtual::17495-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000178225virtual::17495-17ec7a7b4-b510-4816-b236-023aaa0754f0virtual::17495-17ec7a7b4-b510-4816-b236-023aaa0754f0virtual::17495-1ORIGINAL26444.pdfapplication/pdf576330https://repositorio.uniandes.edu.co/bitstreams/31aed4d9-b2f7-41fd-a689-854a6d916558/download33a82ee0137b0937f52441fd21193450MD51TEXT26444.pdf.txt26444.pdf.txtExtracted texttext/plain60551https://repositorio.uniandes.edu.co/bitstreams/e8d84751-c3fb-4b38-891d-575db997ab16/download73277a3aa25bff072389bd18a0a52d54MD52THUMBNAIL26444.pdf.jpg26444.pdf.jpgIM Thumbnailimage/jpeg22959https://repositorio.uniandes.edu.co/bitstreams/86add8fb-6c3e-4123-b72f-9e837ccc179b/downloada42126c1380e416d89768ffe1f8a319cMD531992/55071oai:repositorio.uniandes.edu.co:1992/550712024-03-13 16:02:15.799http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co

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