Uso de anticuerpos como herramienta diagnóstica en queratitis infecciosa por hongos y Acanthamoeba SPP. Revisión sistemática

El propósito es identificar a través de una revisión sistemática de la literatura la evidencia científica disponible sobre el uso de anticuerpos monoclonales y policlonales como herramienta diagnóstica de queratitis por Acanthamoeba y hongos. Una búsqueda de la literatura fue realizada en cuatro bas...

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Autores:
Pérez Riaño, Brian David
Tipo de recurso:
https://purl.org/coar/resource_type/c_7a1f
Fecha de publicación:
0022
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
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spa
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oai:repositorio.unbosque.edu.co:20.500.12495/13747
Acceso en línea:
https://hdl.handle.net/20.500.12495/13747
Palabra clave:
Acanthamoeba
Hongos
Queratitis
Diagnóstico
Anticuerpos
Acanthamoeba
Fungi
Keratitis
Diagnosis
Antibodies
WW 100
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id UNBOSQUE2_ecac718e443152f77ce197318fff1d62
oai_identifier_str oai:repositorio.unbosque.edu.co:20.500.12495/13747
network_acronym_str UNBOSQUE2
network_name_str Repositorio U. El Bosque
repository_id_str
dc.title.none.fl_str_mv Uso de anticuerpos como herramienta diagnóstica en queratitis infecciosa por hongos y Acanthamoeba SPP. Revisión sistemática
dc.title.translated.none.fl_str_mv Use of antibodies as a diagnostic tool in infectious keratitis caused by Fungi and Acanthamoeba SPP. Systematic review
title Uso de anticuerpos como herramienta diagnóstica en queratitis infecciosa por hongos y Acanthamoeba SPP. Revisión sistemática
spellingShingle Uso de anticuerpos como herramienta diagnóstica en queratitis infecciosa por hongos y Acanthamoeba SPP. Revisión sistemática
Acanthamoeba
Hongos
Queratitis
Diagnóstico
Anticuerpos
Acanthamoeba
Fungi
Keratitis
Diagnosis
Antibodies
WW 100
title_short Uso de anticuerpos como herramienta diagnóstica en queratitis infecciosa por hongos y Acanthamoeba SPP. Revisión sistemática
title_full Uso de anticuerpos como herramienta diagnóstica en queratitis infecciosa por hongos y Acanthamoeba SPP. Revisión sistemática
title_fullStr Uso de anticuerpos como herramienta diagnóstica en queratitis infecciosa por hongos y Acanthamoeba SPP. Revisión sistemática
title_full_unstemmed Uso de anticuerpos como herramienta diagnóstica en queratitis infecciosa por hongos y Acanthamoeba SPP. Revisión sistemática
title_sort Uso de anticuerpos como herramienta diagnóstica en queratitis infecciosa por hongos y Acanthamoeba SPP. Revisión sistemática
dc.creator.fl_str_mv Pérez Riaño, Brian David
dc.contributor.advisor.none.fl_str_mv Isaza Gómez, Luis Guillerno
Yaacov Peña, Fernando
dc.contributor.author.none.fl_str_mv Pérez Riaño, Brian David
dc.contributor.orcid.none.fl_str_mv Pérez Riaño, Brian David [0000-0002-2256-4363]
dc.subject.none.fl_str_mv Acanthamoeba
Hongos
Queratitis
Diagnóstico
Anticuerpos
topic Acanthamoeba
Hongos
Queratitis
Diagnóstico
Anticuerpos
Acanthamoeba
Fungi
Keratitis
Diagnosis
Antibodies
WW 100
dc.subject.keywords.none.fl_str_mv Acanthamoeba
Fungi
Keratitis
Diagnosis
Antibodies
dc.subject.nlm.none.fl_str_mv WW 100
description El propósito es identificar a través de una revisión sistemática de la literatura la evidencia científica disponible sobre el uso de anticuerpos monoclonales y policlonales como herramienta diagnóstica de queratitis por Acanthamoeba y hongos. Una búsqueda de la literatura fue realizada en cuatro bases de datos especializadas, PubMed, Embase, Web Of Science y ScienceDirect usando palabras claves como “Acanthamoeba”, “hongos”, “queratitis”, “diagnóstico” y “anticuerpos”, en artículos publicados en inglés y en español durante los últimos 10 años (2014 – 2024). El análisis incluyó once estudios, abarcando investigaciones de Corea del Sur, Japón, Brasil e India. Anticuerpos policlonales y monoclonales detectaron antígenos específicos de Acanthamoeba spp y Asperguillus spp. ELISA, Western Blot y citometría evidenciaron alta especificidad y sensibilidad en investigaciones in vitro, mientras que dispositivos como el AspLFD y el kit FICGA demostraron resultados similares cuando fueron comparados con estudios convencionales. El uso de anticuerpos para el diagnóstico de queratitis por Acanthamoeba spp, presentó alta sensibilidad y especificidad sin evidencia de reactividad cruzada en estudios in vitro. Herramientas como el dispositivo AspLFD y el kit FICGA ofrecieron diagnósticos precisos y más rápidos. Sin embargo, se requiere de validación de estos resultados con futuras investigaciones en diversos contextos clínicos.
publishDate 0022
dc.date.issued.none.fl_str_mv 0022-11
dc.date.accessioned.none.fl_str_mv 2025-01-20T17:26:55Z
dc.date.available.none.fl_str_mv 2025-01-20T17:26:55Z
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
dc.type.local.spa.fl_str_mv Tesis/Trabajo de grado - Monografía - Especialización
dc.type.coar.none.fl_str_mv https://purl.org/coar/resource_type/c_7a1f
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/bachelorThesis
dc.type.coarversion.none.fl_str_mv https://purl.org/coar/version/c_ab4af688f83e57aa
format https://purl.org/coar/resource_type/c_7a1f
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12495/13747
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
dc.identifier.repourl.none.fl_str_mv repourl:https://repositorio.unbosque.edu.co
url https://hdl.handle.net/20.500.12495/13747
identifier_str_mv instname:Universidad El Bosque
reponame:Repositorio Institucional Universidad El Bosque
repourl:https://repositorio.unbosque.edu.co
dc.language.iso.fl_str_mv spa
language spa
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spelling Isaza Gómez, Luis GuillernoYaacov Peña, FernandoPérez Riaño, Brian DavidPérez Riaño, Brian David [0000-0002-2256-4363]2025-01-20T17:26:55Z2025-01-20T17:26:55Z0022-11https://hdl.handle.net/20.500.12495/13747instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coEl propósito es identificar a través de una revisión sistemática de la literatura la evidencia científica disponible sobre el uso de anticuerpos monoclonales y policlonales como herramienta diagnóstica de queratitis por Acanthamoeba y hongos. Una búsqueda de la literatura fue realizada en cuatro bases de datos especializadas, PubMed, Embase, Web Of Science y ScienceDirect usando palabras claves como “Acanthamoeba”, “hongos”, “queratitis”, “diagnóstico” y “anticuerpos”, en artículos publicados en inglés y en español durante los últimos 10 años (2014 – 2024). El análisis incluyó once estudios, abarcando investigaciones de Corea del Sur, Japón, Brasil e India. Anticuerpos policlonales y monoclonales detectaron antígenos específicos de Acanthamoeba spp y Asperguillus spp. ELISA, Western Blot y citometría evidenciaron alta especificidad y sensibilidad en investigaciones in vitro, mientras que dispositivos como el AspLFD y el kit FICGA demostraron resultados similares cuando fueron comparados con estudios convencionales. El uso de anticuerpos para el diagnóstico de queratitis por Acanthamoeba spp, presentó alta sensibilidad y especificidad sin evidencia de reactividad cruzada en estudios in vitro. Herramientas como el dispositivo AspLFD y el kit FICGA ofrecieron diagnósticos precisos y más rápidos. Sin embargo, se requiere de validación de estos resultados con futuras investigaciones en diversos contextos clínicos.Especialista en OftalmologíaEspecializaciónThe porpuse is to identify, through a systematic literature review, the available scientific evidence on the use of monoclonal and polyclonal antibodies as diagnostic tool for Acanthamoeba and fungal keratitis. A literature search was conducted in four specialized databases: PubMed, Embase, Web of Science and ScienceDirect, using keywords such as “Acanthamoeba”, “fungi”, “keratitis”, “diagnosis” and “antibodies”, in articles published in English and Spanish over the last 10 years (2014-2024). The analysis included eleven studies, including research from South Korea, Japan, Brazil, and India. Polyclonal and monoclonal antibodies detected specific antigens of Acanthamoeba spp., and Asperguillus spp. ELISA, Western Blot and flow cytometry demostrated high specificity and sensitivity in in vitro investigations, while devices such as the AspLFD and the FICGA kit demostrated similar results when compared to conventional studies. The use of antibodies for the diagnosis of Acanthamoeba ssp keratitis showed high sensitivity and specificity with no evidence of cross-reactivity in in vitro studies. Tools such as the AspLFD device and the FICGA kit offered accurate and faster diagnosis. However, validation of these results is needed through future research in different clinical context.application/pdfAttribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/Acceso abiertohttps://purl.org/coar/access_right/c_abf2http://purl.org/coar/access_right/c_abf2AcanthamoebaHongosQueratitisDiagnósticoAnticuerposAcanthamoebaFungiKeratitisDiagnosisAntibodiesWW 100Uso de anticuerpos como herramienta diagnóstica en queratitis infecciosa por hongos y Acanthamoeba SPP. Revisión sistemáticaUse of antibodies as a diagnostic tool in infectious keratitis caused by Fungi and Acanthamoeba SPP. Systematic reviewEspecialización en OftalmologíaUniversidad El BosqueFacultad de MedicinaTesis/Trabajo de grado - Monografía - Especializaciónhttps://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesishttps://purl.org/coar/version/c_ab4af688f83e57aaCabrera-Aguas MBBS M, Khoo P, Watson SL. Infectious keratitis: A review. Clin Exp Ophthalmol. 2022;50(5):543–62.McCormick I, James C, Welton NJ, Mayaud P, Turner KME, Gottlieb SL, et al. Incidence of herpes simplex virus keratitis and other ocular disease: global review and estimates [Internet]. Vol. 29, Ophthalmic Epidemiology. Ophthalmic Epidemiol; 2022 [cited 2024 Nov 11]. p. 353–62. Available from: https://pubmed.ncbi.nlm.nih.gov/34622738/Kim MJ, Jo HJ, Sohn HJ, Shin HJ, Quan FS, Kong HH, et al. Evaluating the Diagnostic Potential of Chorismate Mutase Poly-Clonal Peptide Antibody for the Acanthamoeba Keratitis in an Animal Model. Pathogens. 2023 Apr 1;12(4).Weber-Lima MM, Prado-Costa B, Becker-Finco A, Costa AO, Billilad P, Furst C, et al. Acanthamoeba spp. monoclonal antibody against a CPA2 transporter: a promising molecular tool for acanthamoebiasis diagnosis and encystment study. Parasitology. 2020 Dec 1;147(14):1678–88.Carnt N, Hoffman JJ, Verma S, Hau S, Radford CF, Minassian DC, et al. Acanthamoeba keratitis: Confirmation of the UK outbreak and a prospective case-control study identifying contributing risk factors. Br J Ophthalmol [Internet]. 2018 Dec 1 [cited 2024 Nov 11];102(12):1621–8. Available from: https://pubmed.ncbi.nlm.nih.gov/30232172/Azzopardi M, Chong YJ, Ng B, Recchioni A, Logeswaran A, Ting DSJ. Diagnosis of Acanthamoeba Keratitis: Past, Present and Future [Internet]. Vol. 13, Diagnostics. 2023 [cited 2024 Oct 25]. Available from: https://doi.org/10.3390/diagnostics13162655Zhang Y, Xu X, Wei Z, Cao K, Zhang Z, Liang Q. The global epidemiology and clinical diagnosis of Acanthamoeba keratitis [Internet]. Vol. 16, Journal of Infection and Public Health. J Infect Public Health; 2023 [cited 2024 Nov 11]. p. 841–52. Available from: https://pubmed.ncbi.nlm.nih.gov/37030037/Lee H, Kim MJ, Chung J, Kim W, Jo HJ, Kim TG, et al. A chorismate mutase-targeted, core-shell nanoassembly-activated SERS immunoassay platform for rapid non-invasive detection of Acanthamoeba infection. Nano Today. 2024 Dec 1;59:102506.Aiello F, Afflitto GG, Ceccarelli F, Turco MV, Han Y, Amescua G, et al. Perspectives on the incidence of Acanthamoeba Keratitis: A Systematic Review and Meta-Analysis. Ophthalmology [Internet]. 2024 Aug [cited 2024 Nov 11];0(0). Available from: http://www.aaojournal.org/article/S0161642024004627/fulltextAwad R, Ghaith AA, Awad K, Saad MM, Elmassry AA. Fungal Keratitis: Diagnosis, Management, and Recent Advances. Clin Ophthalmol [Internet]. 2024 [cited 2024 Oct 31];18:85. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC10788054/Sadik N, Elzeiny SM, Ali YE, Sobeih D. Fungal Keratitis in the Egyptian Delta: Epidemiology, Risk Factors, and Microbiological Diagnosis. Ophthalmic Epidemiol [Internet]. 2022 Mar 4 [cited 2024 Nov 11];29(2):198–205. 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