Semicontinuous system for the production of recombinant mCherry protein in Chlamydomonas reinhardtii
Biotechnology advances have allowed bacteria, yeasts, plants, mammalian and insect cells to function as heterologous protein expression systems. Recently, microalgae have gained attention as an innovative platform for recombinant protein production, due to low culture media cost, compared to traditi...
- Autores:
-
Diaz Arias, Cesar Andres
Matsudo, Marcelo Chuei
Ferreira-Camargo, Livia Seno
Dutra Molino, João Vitor
Mayfield, Stephen Patrick
Monteiro de Carvalho, João Carlos
- Tipo de recurso:
- https://purl.org/coar/resource_type/c_6501
- Fecha de publicación:
- 2021
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/5885
- Palabra clave:
- Biotechnology
Bubble column photobioreactor
Heterologous protein
Microalgae cultivation
- Rights
- License
- Acceso abierto
| Summary: | Biotechnology advances have allowed bacteria, yeasts, plants, mammalian and insect cells to function as heterologous protein expression systems. Recently, microalgae have gained attention as an innovative platform for recombinant protein production, due to low culture media cost, compared to traditional systems, as well as the fact that microalgae such as Chlamydomonas reinhardtii are considered safe (GRAS) by the Food and Drug Administration (FDA). Previous studies showed that recombinant protein production in traditional platforms by semicontinuous process increased biomass and bio product productivity, when compared to batch process. As there is a lack of studies on semicontinuous process for recombinant protein production in microalgae, the production of recombinant mCherry fluorescent protein was evaluated by semicontinuous cultivation of Chlamydomonas reinhardtii in bubble column photobioreactor. This semicontinuous cultivation process was evaluated in the following conditions: 20%, 40%, and 60% culture portion withdrawal. The highest culture withdrawal percentage (60%) provided the best results, as an up to 161% increase in mCherry productivity (454.5 RFU h−1 – Relative Fluorescence Unit h−1), in comparison to batch cultivation (174.0 RFU h−1) of the same strain. All cultivations were carried out for 13 days, at pH 7, temperature 25°C and, by semicontinuous process, two culture withdrawals were taken during the cultivations. Throughout the production cycles, it was possible to obtain biomass concentration up to 1.36 g L−1. |
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