In vitro callogenesis induction of Guarianthe skinneri (Bateman) Dressler and W.E. Higgins (Orchidaceae)

Guarianthe skinneri (Bateman) Dressler and W.E. Higgins, is a native orchid from Mexico, considered as threatened species in the NOM-ECOL-059-SEMARNAT-2010, mainly due to the disappearance of its natural habitat and illegal collection during the flowering season. The aim of this research was to induce in vitro callogenesis using different types of explants and phytoregulators, in order to establish a mass production technique as a contribution to the conservation of this species. We evaluated leaf and pseudobulb pith explants growing in semi-solid medium MS, adding BAP, 2, 4-D, Kin, the combination of BAP/2 and 4-D/Kin/Sad and a control without any type of plant growth regulators. Statistical analysis showed that pseudobulb pith explants are more suitable for in vitro callus induction in comparison to leaf explants, because of a lower percentage of contamination (18.8% in pith and 73.2% in leaves). Likewise, the pseudobulb pith explants showed increased callus formation (10.8%) in comparison to leaf explants (7.6%). Regarding the phytoregulators employed, BAP promoted increased callus formation (17%) compared to other phytoregulators (7-10%). This is the first report of the use of pseudobulb pith as an explant for callus induction in G. skinneri.

• Autores: Coutiño-Cortés, Ana Gabriela; Bertolini, Vincenzo; Iracheta Donjuan, Leobardo; Ruíz-Montoya, Lorena; Valle-Mora, Javier Francisco
• Tipo de recurso: Article of journal
• Fecha de publicación: 2017
• Institución: Universidad Nacional de Colombia
• Repositorio: Universidad Nacional de Colombia
• Idioma: spa
• OAI Identifier: oai:repositorio.unal.edu.co:unal/61102
• Acceso en línea: https://repositorio.unal.edu.co/handle/unal/61102; http://bdigital.unal.edu.co/59910/
• Palabra clave: 55 Ciencias de la tierra / Earth sciences and geology; 63 Agricultura y tecnologías relacionadas / Agriculture; Biodiversity conservation; cell division; oxidation; phytoregulator; Biodiversity conservation; cell division; oxidation; phytoregulator
• Rights: openAccess
• License: Atribución-NoComercial 4.0 Internacional