Protopectinase-se from Geotrichumklebahnii: Studies of the adsorption and pectin-solubilization capacity

Protopectinase SE (PPase-SE) is a polygalacturonase produced by Geotrichumklebahnii with the capacity to liberate pectin through protopectin hydrolysis. The protopectin cleavage is a typical heterogeneous-catalysis reaction whose interaction between the enzyme and the protopectin substrate from lemon albedo along with the release of the pectin-reaction product were the objectives of this investigation. The interaction between PPase-SE and protopectin depended on the particle size and the structure of the substrate as well as on the nature of the buffer. The adsorption kinetics follows, for small particles, a Langmuir isotherm pattern. The reaction exhibited Michaelis-Menten kinetics, giving respective apparent-Km and Vmax values of 30.2 g/l and 57.3 g/l.h. The better results in enzyme adsorption and pectin releasing were obtained with citrate and citrate-phosphate buffers. This report constitutes the first investigation in pectin solubilization involving a model for the substrate-binding mechanism within the pectinase-protopectinase system.

• Autores: Zapata Zapata, Arley David; Hours, Roque Alberto; Cavalitto, Sebastian Fernando
• Tipo de recurso: Article of journal
• Fecha de publicación: 2017
• Institución: Universidad Nacional de Colombia
• Repositorio: Universidad Nacional de Colombia
• Idioma: spa
• OAI Identifier: oai:repositorio.unal.edu.co:unal/60379
• Acceso en línea: https://repositorio.unal.edu.co/handle/unal/60379; http://bdigital.unal.edu.co/58711/
• Palabra clave: 62 Ingeniería y operaciones afines / Engineering; Pectin; protopectin; protopectinase-SE; Langmuir isotherm; Michaelis-Menten; Pectina; protopectina; protopectinasa-SE; Isoterma de Langmuir; Michaelis-Menten
• Rights: openAccess
• License: Atribución-NoComercial 4.0 Internacional