Optimización de las condiciones de fermentación líquida en biorreactor para la producción de Lentinula edodes

ilustraciones, gráficas, tablas

Autores:: Bello Forero, Robinson Augusto

Tipo de recurso:

Fecha de publicación:: 2021

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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oai_identifier_str	oai:repositorio.unal.edu.co:unal/80126
network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Optimización de las condiciones de fermentación líquida en biorreactor para la producción de Lentinula edodes
dc.title.translated.eng.fl_str_mv	Optimization of culture conditions in a bioreactor for the production of biomass and metabolites of the macromycete Lentinus edodes
title	Optimización de las condiciones de fermentación líquida en biorreactor para la producción de Lentinula edodes
spellingShingle	Optimización de las condiciones de fermentación líquida en biorreactor para la producción de Lentinula edodes 660 - Ingeniería química 620 - Ingeniería y operaciones afines Antioxidantes Biomasa Biomass Antioxidants Fermentación en estado líquido (FEL) Macromiceto Lentinula edodes Polisacáridos Esteroles Liquid State Fermentation Macromicete Lentinus edodes Steroles Polysaccharides
title_short	Optimización de las condiciones de fermentación líquida en biorreactor para la producción de Lentinula edodes
title_full	Optimización de las condiciones de fermentación líquida en biorreactor para la producción de Lentinula edodes
title_fullStr	Optimización de las condiciones de fermentación líquida en biorreactor para la producción de Lentinula edodes
title_full_unstemmed	Optimización de las condiciones de fermentación líquida en biorreactor para la producción de Lentinula edodes
title_sort	Optimización de las condiciones de fermentación líquida en biorreactor para la producción de Lentinula edodes
dc.creator.fl_str_mv	Bello Forero, Robinson Augusto
dc.contributor.advisor.none.fl_str_mv	Serrato Bermudez, Juan Carlos Chegwin, Carolina
dc.contributor.author.none.fl_str_mv	Bello Forero, Robinson Augusto
dc.contributor.researchgroup.spa.fl_str_mv	Procesos Químicos y Bioquímicos Química de Hongos Macromicetos
dc.subject.ddc.spa.fl_str_mv	660 - Ingeniería química 620 - Ingeniería y operaciones afines
topic	660 - Ingeniería química 620 - Ingeniería y operaciones afines Antioxidantes Biomasa Biomass Antioxidants Fermentación en estado líquido (FEL) Macromiceto Lentinula edodes Polisacáridos Esteroles Liquid State Fermentation Macromicete Lentinus edodes Steroles Polysaccharides
dc.subject.armarc.spa.fl_str_mv	Antioxidantes
dc.subject.lemb.spa.fl_str_mv	Biomasa
dc.subject.lemb.eng.fl_str_mv	Biomass Antioxidants
dc.subject.proposal.spa.fl_str_mv	Fermentación en estado líquido (FEL) Macromiceto Lentinula edodes Polisacáridos
dc.subject.proposal.none.fl_str_mv	Esteroles
dc.subject.proposal.eng.fl_str_mv	Liquid State Fermentation Macromicete Lentinus edodes Steroles Polysaccharides
description	ilustraciones, gráficas, tablas
publishDate	2021
dc.date.accessioned.none.fl_str_mv	2021-09-08T04:06:53Z
dc.date.available.none.fl_str_mv	2021-09-08T04:06:53Z
dc.date.issued.none.fl_str_mv	2021-06
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/80126
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/80126 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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spelling	Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Serrato Bermudez, Juan Carlos595155aadb20cd0440a67ad2b96ce2e4Chegwin, Carolinad68b4f79b8ee691437e6041b3b049ae6600Bello Forero, Robinson Augustobf1b3ee42cf42d433ae3ebe716ab870a600Procesos Químicos y BioquímicosQuímica de Hongos Macromicetos2021-09-08T04:06:53Z2021-09-08T04:06:53Z2021-06https://repositorio.unal.edu.co/handle/unal/80126Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, gráficas, tablasEsta investigación consistió en la determinación del efecto y la optimización de algunas de las condiciones de la fermentación en estado líquido en un biorreactor sobre la producción de biomasa y la composición del macromiceto Lentinula edodes. Inicialmente, se evalúo el efecto de la concentración de glucosa en el medio de cultivo, la aireación y la agitación mediante un diseño Box-Behnken, y se determinó que tanto la concentración de glucosa como la agitación tuvieron un efecto significativo sobre las variables de respuesta (biomasa, esteroles y polisacáridos). Así mismo se determinó que bajo las condiciones evaluadas, los valores de los variables que optimizaron la producción de biomasa fueron de 1.2 vvm, 60 rpm y 21.97 g/L de glucosa. Posteriormente, se cuantificaron los esteroles y carbohidratos totales extraídos de la biomasa a través de un análisis espectrofotométrico, encontrando que para optimizar estas dos variables de respuesta se requieren valores similares para la aireación (1.2 vvm) y la agitación (60 rpm), mientras que la concentración de glucosa fue de 16.32 g/L y 19.6 g/L para esteroles y polisacáridos totales respectivamente. Finalmente, se realizó un estudio del perfil de los extractos mediante HPLC-DAD y GC-MS, encontrando que la concentración de glucosa afecta el número de señales cromatográficas y la abundancia de los diferentes grupos de metabolitos de interés. (Texto tomado de la fuente)This research used an experimental design for optimizing and determining some of the submerged culture conditions effect over Lentinus edodes biomass production and its composition. To start with, glucose concentration, aeration and agitation effect were evaluated in the culture medium using a Box-Behnken design, which, allows concluding glucose concentration and agitation had a significant effect over the response variables (biomass, sterols and polysaccharides). Also, it was figured out that 1.2 vvm, 60 rpm and 21.97g / L were the values for aeration, agitation, and glucose concentration respectively for biomass production optimization. Subsequently, sterols and total carbohydrates were extracted from biomass and quantified through a spectrophotometric analysis, which, allows to determine that although these response variables were optimized using the same values for aeration and agitation obtained from the biomass production optimization, the glucose concentration was different (16.32 g / L for sterols and 19.6 g / L for total polysaccharides). Finally, the extracts profile was analyzed using HPLC-DAD and GC-MS. It was found that glucose affects the number of chromatographic signals and the quantity of different metabolites groups.MaestríaMagíster en Ingeniería - Ingeniería QuímicaBioprocesosxv, 109 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ingeniería - Maestría en Ingeniería - Ingeniería QuímicaDepartamento de Ingeniería Química y AmbientalFacultad de IngenieríaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá660 - Ingeniería química620 - Ingeniería y operaciones afinesAntioxidantesBiomasaBiomassAntioxidantsFermentación en estado líquido (FEL)MacromicetoLentinula edodesPolisacáridosEsterolesLiquid State FermentationMacromiceteLentinus edodesSterolesPolysaccharidesOptimización de las condiciones de fermentación líquida en biorreactor para la producción de Lentinula edodesOptimization of culture conditions in a bioreactor for the production of biomass and metabolites of the macromycete Lentinus edodesTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMA Pandey, CR Soccol, JA Rodriguez-Leon, P. 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World Journal of Microbiology and Biotechnology, 21(6–7), 1267–1271. https://doi.org/10.1007/s11274-005-1941-5Optimización de las condiciones de fermentación líquida en biorreactor para la producción de Lentinula edodesOptimization of the submerged culture conditions in a bioreactor for the production of Lentinus edodesPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-83964https://repositorio.unal.edu.co/bitstream/unal/80126/1/license.txtcccfe52f796b7c63423298c2d3365fc6MD51ORIGINAL11275357.2021.pdf11275357.2021.pdfTesis de Magister en Ingeniería - Ingeniería Químicaapplication/pdf3538902https://repositorio.unal.edu.co/bitstream/unal/80126/2/11275357.2021.pdfe70842f7e8f3c959a3e538b71566e7efMD52THUMBNAIL11275357.2021.pdf.jpg11275357.2021.pdf.jpgGenerated Thumbnailimage/jpeg5224https://repositorio.unal.edu.co/bitstream/unal/80126/3/11275357.2021.pdf.jpgb6d718a9d09f8c6fcb116b003cc12730MD53unal/80126oai:repositorio.unal.edu.co:unal/801262023-07-27 23:03:47.035Repositorio 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Colombiarepositorio_nal@unal.edu.coUExBTlRJTExBIERFUMOTU0lUTwoKQ29tbyBlZGl0b3IgZGUgZXN0ZSDDrXRlbSwgdXN0ZWQgcHVlZGUgbW92ZXJsbyBhIHJldmlzacOzbiBzaW4gYW50ZXMgcmVzb2x2ZXIgbG9zIHByb2JsZW1hcyBpZGVudGlmaWNhZG9zLCBkZSBsbyBjb250cmFyaW8sIGhhZ2EgY2xpYyBlbiBHdWFyZGFyIHBhcmEgZ3VhcmRhciBlbCDDrXRlbSB5IHNvbHVjaW9uYXIgZXN0b3MgcHJvYmxlbWFzIG1hcyB0YXJkZS4KCk5PVEFTOgoqU0kgTEEgVEVTSVMgQSBQVUJMSUNBUiBBRFFVSVJJw5MgQ09NUFJPTUlTT1MgREUgQ09ORklERU5DSUFMSURBRCBFTiBFTCBERVNBUlJPTExPIE8gUEFSVEVTIERFTCBET0NVTUVOVE8uIFNJR0EgTEEgRElSRUNUUklaIERFIExBIFJFU09MVUNJw5NOIDAyMyBERSAyMDE1LCBQT1IgTEEgQ1VBTCBTRSBFU1RBQkxFQ0UgRUwgUFJPQ0VESU1JRU5UTyBQQVJBIExBIFBVQkxJQ0FDScOTTiBERSBURVNJUyBERSBNQUVTVFLDjUEgWSBET0NUT1JBRE8gREUgTE9TIEVTVFVESUFOVEVTIERFIExBIFVOSVZFUlNJREFEIE5BQ0lPTkFMIERFIENPTE9NQklBIEVOIEVMIFJFUE9TSVRPUklPIElOU1RJVFVDSU9OQUwgVU4sIEVYUEVESURBIFBPUiBMQSBTRUNSRVRBUsONQSBHRU5FUkFMLgoqTEEgVEVTSVMgQSBQVUJMSUNBUiBERUJFIFNFUiBMQSBWRVJTScOTTiBGSU5BTCBBUFJPQkFEQS4KUGFyYSB0cmFiYWpvcyBkZXBvc2l0YWRvcyBwb3Igc3UgcHJvcGlvIGF1dG9yOiBBbCBhdXRvYXJjaGl2YXIgZXN0ZSBncnVwbyBkZSBhcmNoaXZvcyBkaWdpdGFsZXMgeSBzdXMgbWV0YWRhdG9zLCBZbyBnYXJhbnRpem8gYWwgUmVwb3NpdG9yaW8gSW5zdGl0dWNpb25hbCBVTiBlbCBkZXJlY2hvIGEgYWxtYWNlbmFybG9zIHkgbWFudGVuZXJsb3MgZGlzcG9uaWJsZXMgZW4gbMOtbmVhIGRlIG1hbmVyYSBncmF0dWl0YS4gRGVjbGFybyBxdWUgZGljaG8gbWF0ZXJpYWwgZXMgZGUgbWkgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgcXVlIGVsIFJlcG9zaXRvcmlvIEluc3RpdHVjaW9uYWwgVU4gbm8gYXN1bWUgbmluZ3VuYSByZXNwb25zYWJpbGlkYWQgc2kgaGF5IGFsZ3VuYSB2aW9sYWNpw7NuIGEgbG9zIGRlcmVjaG9zIGRlIGF1dG9yIGFsIGRpc3RyaWJ1aXIgZXN0b3MgYXJjaGl2b3MgeSBtZXRhZGF0b3MuIChTZSByZWNvbWllbmRhIGEgdG9kb3MgbG9zIGF1dG9yZXMgYSBpbmRpY2FyIHN1cyBkZXJlY2hvcyBkZSBhdXRvciBlbiBsYSBww6FnaW5hIGRlIHTDrXR1bG8gZGUgc3UgZG9jdW1lbnRvLikgRGUgbGEgbWlzbWEgbWFuZXJhLCBhY2VwdG8gbG9zIHTDqXJtaW5vcyBkZSBsYSBzaWd1aWVudGUgbGljZW5jaWE6IExvcyBhdXRvcmVzIG8gdGl0dWxhcmVzIGRlbCBkZXJlY2hvIGRlIGF1dG9yIGRlbCBwcmVzZW50ZSBkb2N1bWVudG8gY29uZmllcmVuIGEgbGEgVW5pdmVyc2lkYWQgTmFjaW9uYWwgZGUgQ29sb21iaWEgdW5hIGxpY2VuY2lhIG5vIGV4Y2x1c2l2YSwgbGltaXRhZGEgeSBncmF0dWl0YSBzb2JyZSBsYSBvYnJhIHF1ZSBzZSBpbnRlZ3JhIGVuIGVsIFJlcG9zaXRvcmlvIEluc3RpdHVjaW9uYWwsIHF1ZSBzZSBhanVzdGEgYSBsYXMgc2lndWllbnRlcyBjYXJhY3RlcsOtc3RpY2FzOiBhKSBFc3RhcsOhIHZpZ2VudGUgYSBwYXJ0aXIgZGUgbGEgZmVjaGEgZW4gcXVlIHNlIGluY2x1eWUgZW4gZWwgcmVwb3NpdG9yaW8sIHF1ZSBzZXLDoW4gcHJvcnJvZ2FibGVzIGluZGVmaW5pZGFtZW50ZSBwb3IgZWwgdGllbXBvIHF1ZSBkdXJlIGVsIGRlcmVjaG8gcGF0cmltb25pYWwgZGVsIGF1dG9yLiBFbCBhdXRvciBwb2Ryw6EgZGFyIHBvciB0ZXJtaW5hZGEgbGEgbGljZW5jaWEgc29saWNpdMOhbmRvbG8gYSBsYSBVbml2ZXJzaWRhZC4gYikgTG9zIGF1dG9yZXMgYXV0b3JpemFuIGEgbGEgVW5pdmVyc2lkYWQgTmFjaW9uYWwgZGUgQ29sb21iaWEgcGFyYSBwdWJsaWNhciBsYSBvYnJhIGVuIGVsIGZvcm1hdG8gcXVlIGVsIHJlcG9zaXRvcmlvIGxvIHJlcXVpZXJhIChpbXByZXNvLCBkaWdpdGFsLCBlbGVjdHLDs25pY28gbyBjdWFscXVpZXIgb3RybyBjb25vY2lkbyBvIHBvciBjb25vY2VyKSB5IGNvbm9jZW4gcXVlIGRhZG8gcXVlIHNlIHB1YmxpY2EgZW4gSW50ZXJuZXQgcG9yIGVzdGUgaGVjaG8gY2lyY3VsYSBjb24gdW4gYWxjYW5jZSBtdW5kaWFsLiBjKSBMb3MgYXV0b3JlcyBhY2VwdGFuIHF1ZSBsYSBhdXRvcml6YWNpw7NuIHNlIGhhY2UgYSB0w610dWxvIGdyYXR1aXRvLCBwb3IgbG8gdGFudG8sIHJlbnVuY2lhbiBhIHJlY2liaXIgZW1vbHVtZW50byBhbGd1bm8gcG9yIGxhIHB1YmxpY2FjacOzbiwgZGlzdHJpYnVjacOzbiwgY29tdW5pY2FjacOzbiBww7pibGljYSB5IGN1YWxxdWllciBvdHJvIHVzbyBxdWUgc2UgaGFnYSBlbiBsb3MgdMOpcm1pbm9zIGRlIGxhIHByZXNlbnRlIGxpY2VuY2lhIHkgZGUgbGEgbGljZW5jaWEgQ3JlYXRpdmUgQ29tbW9ucyBjb24gcXVlIHNlIHB1YmxpY2EuIGQpIExvcyBhdXRvcmVzIG1hbmlmaWVzdGFuIHF1ZSBzZSB0cmF0YSBkZSB1bmEgb2JyYSBvcmlnaW5hbCBzb2JyZSBsYSBxdWUgdGllbmVuIGxvcyBkZXJlY2hvcyBxdWUgYXV0b3JpemFuIHkgcXVlIHNvbiBlbGxvcyBxdWllbmVzIGFzdW1lbiB0b3RhbCByZXNwb25zYWJpbGlkYWQgcG9yIGVsIGNvbnRlbmlkbyBkZSBzdSBvYnJhIGFudGUgbGEgVW5pdmVyc2lkYWQgTmFjaW9uYWwgeSBhbnRlIHRlcmNlcm9zLiBFbiB0b2RvIGNhc28gbGEgVW5pdmVyc2lkYWQgTmFjaW9uYWwgZGUgQ29sb21iaWEgc2UgY29tcHJvbWV0ZSBhIGluZGljYXIgc2llbXByZSBsYSBhdXRvcsOtYSBpbmNsdXllbmRvIGVsIG5vbWJyZSBkZWwgYXV0b3IgeSBsYSBmZWNoYSBkZSBwdWJsaWNhY2nDs24uIGUpIExvcyBhdXRvcmVzIGF1dG9yaXphbiBhIGxhIFVuaXZlcnNpZGFkIHBhcmEgaW5jbHVpciBsYSBvYnJhIGVuIGxvcyDDrW5kaWNlcyB5IGJ1c2NhZG9yZXMgcXVlIGVzdGltZW4gbmVjZXNhcmlvcyBwYXJhIHByb21vdmVyIHN1IGRpZnVzacOzbi4gZikgTG9zIGF1dG9yZXMgYWNlcHRhbiBxdWUgbGEgVW5pdmVyc2lkYWQgTmFjaW9uYWwgZGUgQ29sb21iaWEgcHVlZGEgY29udmVydGlyIGVsIGRvY3VtZW50byBhIGN1YWxxdWllciBtZWRpbyBvIGZvcm1hdG8gcGFyYSBwcm9ww7NzaXRvcyBkZSBwcmVzZXJ2YWNpw7NuIGRpZ2l0YWwuIFNJIEVMIERPQ1VNRU5UTyBTRSBCQVNBIEVOIFVOIFRSQUJBSk8gUVVFIEhBIFNJRE8gUEFUUk9DSU5BRE8gTyBBUE9ZQURPIFBPUiBVTkEgQUdFTkNJQSBPIFVOQSBPUkdBTklaQUNJw5NOLCBDT04gRVhDRVBDScOTTiBERSBMQSBVTklWRVJTSURBRCBOQUNJT05BTCBERSBDT0xPTUJJQSwgTE9TIEFVVE9SRVMgR0FSQU5USVpBTiBRVUUgU0UgSEEgQ1VNUExJRE8gQ09OIExPUyBERVJFQ0hPUyBZIE9CTElHQUNJT05FUyBSRVFVRVJJRE9TIFBPUiBFTCBSRVNQRUNUSVZPIENPTlRSQVRPIE8gQUNVRVJETy4KUGFyYSB0cmFiYWpvcyBkZXBvc2l0YWRvcyBwb3Igb3RyYXMgcGVyc29uYXMgZGlzdGludGFzIGEgc3UgYXV0b3I6IERlY2xhcm8gcXVlIGVsIGdydXBvIGRlIGFyY2hpdm9zIGRpZ2l0YWxlcyB5IG1ldGFkYXRvcyBhc29jaWFkb3MgcXVlIGVzdG95IGFyY2hpdmFuZG8gZW4gZWwgUmVwb3NpdG9yaW8gSW5zdGl0dWNpb25hbCBVTikgZXMgZGUgZG9taW5pbyBww7pibGljby4gU2kgbm8gZnVlc2UgZWwgY2FzbywgYWNlcHRvIHRvZGEgbGEgcmVzcG9uc2FiaWxpZGFkIHBvciBjdWFscXVpZXIgaW5mcmFjY2nDs24gZGUgZ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Optimización de las condiciones de fermentación líquida en biorreactor para la producción de Lentinula edodes

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