Discovery of a Protective Rickettsia prowazekii Antigen Recognized by CD8+ T Cells, RP884, Using an In Vivo Screening Plataform

ABSTRACT: Rickettsia prowazekii has been tested for biological warfare due to the high mortality that it produces after aerosol transmission of very low numbers of rickettsiae. Epidemic typhus, the infection caused by these obligately intracellular bacteria, continues to be a threat because it is difficult to diagnose due to initial non-specific symptoms and the lack of commercial diagnostic tests that are sensitive and specific during the initial clinical presentation. A vaccine to prevent epidemic typhus would constitute an effective deterrent to the weaponization of R. prowazekii; however, an effective and safe vaccine is not currently available. Due to the cytoplasmic niche of Rickettsia, CD8+ T-cells are critical effectors of immunity; however, the identification of antigens recognized by these cells has not been systematically addressed. To help close this gap, we designed an antigen discovery strategy that uses cell-based vaccination with antigen presenting cells expressing microbe's proteins targeted to the MHC class I presentation pathway. We report the use of this method to discover a protective T-cell rickettsial antigen, RP884, among a test subset of rickettsial proteins.

• Autores: Correa Vanegas, Paula Andrea; Gazi, Michal; Caro Gómez, Erika; Goez, Yenny; Céspedes, María A.; Hidalgo, Marilyn; Valbuena, Gustavo
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2013
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/39562
• Acceso en línea: https://hdl.handle.net/10495/39562
• Palabra clave: Células Presentadoras de Antígenos; Antigen-Presenting Cells; Antígenos Bacterianos; Antigens, Bacterial; Proteínas Bacterianas; Bacterial Proteins; Linfocitos T CD8-positivos; CD8-Positive T-Lymphocytes; Biología Computacional; Computational Biology; Regulación Bacteriana de la Expresión Génica; Gene Expression Regulation, Bacterial; Genes Bacterianos; Genes, Bacterial; Vectores Genéticos; Genetic Vectors; Rickettsia prowazekii; Vacunas contra Rickettsia; Rickettsial Vaccines; Tifus Epidémico Transmitido por Piojos; Typhus, Epidemic Louse-Borne; https://id.nlm.nih.gov/mesh/D000938; https://id.nlm.nih.gov/mesh/D000942; https://id.nlm.nih.gov/mesh/D001426; https://id.nlm.nih.gov/mesh/D018414; https://id.nlm.nih.gov/mesh/D019295; https://id.nlm.nih.gov/mesh/D015964; https://id.nlm.nih.gov/mesh/D005798; https://id.nlm.nih.gov/mesh/D005822; https://id.nlm.nih.gov/mesh/D012283; https://id.nlm.nih.gov/mesh/D012290; https://id.nlm.nih.gov/mesh/D014438
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/2.5/co/