Rapid differentiation between Livestock-Associated and Livestock-Independent Staphylococcus aureus CC398 Clades

ABSTRACT: Staphylococcus aureus clonal complex 398 (CC398) isolates cluster into two distinct phylogenetic clades based on singlenucleotide polymorphisms (SNPs) revealing a basal human clade and a more derived livestock clade. The scn and tet(M) genes are strongly associated with the human and the l...

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Autores:
Stegger, Marc
Liu, Cindy M.
Larsen, Jesper
Soldanova, Katerina
Aziz, Maliha
Contente Cuomo, Tania
Petersen, Andreas
Vandendriessche, Stien
Jiménez Quiceno, Judy Natalia
Mammina, Caterina
Belkum, Alex Van
Salmenlinna, Saara
Laurent, Frederic
Skov, Robert L.
Larsen, Anders R.
Andersen, Paal S.
Price, Lance B.
Tipo de recurso:
Article of investigation
Fecha de publicación:
2013
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/22136
Acceso en línea:
http://hdl.handle.net/10495/22136
Palabra clave:
Staphylococcus aureus
Filogenia
Phylogeny
Polimorfismo genético
Genetic polymorphism
Polimorfismo de un solo nucleótido
Single nucleotide polymorphism
http://aims.fao.org/aos/agrovoc/c_24031
http://aims.fao.org/aos/agrovoc/c_0273c413
Rights
openAccess
License
http://creativecommons.org/licenses/by/2.5/co/
id UDEA2_fc1c3c0fd7724450f1444a56ed7a17fe
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/22136
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Rapid differentiation between Livestock-Associated and Livestock-Independent Staphylococcus aureus CC398 Clades
title Rapid differentiation between Livestock-Associated and Livestock-Independent Staphylococcus aureus CC398 Clades
spellingShingle Rapid differentiation between Livestock-Associated and Livestock-Independent Staphylococcus aureus CC398 Clades
Staphylococcus aureus
Filogenia
Phylogeny
Polimorfismo genético
Genetic polymorphism
Polimorfismo de un solo nucleótido
Single nucleotide polymorphism
http://aims.fao.org/aos/agrovoc/c_24031
http://aims.fao.org/aos/agrovoc/c_0273c413
title_short Rapid differentiation between Livestock-Associated and Livestock-Independent Staphylococcus aureus CC398 Clades
title_full Rapid differentiation between Livestock-Associated and Livestock-Independent Staphylococcus aureus CC398 Clades
title_fullStr Rapid differentiation between Livestock-Associated and Livestock-Independent Staphylococcus aureus CC398 Clades
title_full_unstemmed Rapid differentiation between Livestock-Associated and Livestock-Independent Staphylococcus aureus CC398 Clades
title_sort Rapid differentiation between Livestock-Associated and Livestock-Independent Staphylococcus aureus CC398 Clades
dc.creator.fl_str_mv Stegger, Marc
Liu, Cindy M.
Larsen, Jesper
Soldanova, Katerina
Aziz, Maliha
Contente Cuomo, Tania
Petersen, Andreas
Vandendriessche, Stien
Jiménez Quiceno, Judy Natalia
Mammina, Caterina
Belkum, Alex Van
Salmenlinna, Saara
Laurent, Frederic
Skov, Robert L.
Larsen, Anders R.
Andersen, Paal S.
Price, Lance B.
dc.contributor.author.none.fl_str_mv Stegger, Marc
Liu, Cindy M.
Larsen, Jesper
Soldanova, Katerina
Aziz, Maliha
Contente Cuomo, Tania
Petersen, Andreas
Vandendriessche, Stien
Jiménez Quiceno, Judy Natalia
Mammina, Caterina
Belkum, Alex Van
Salmenlinna, Saara
Laurent, Frederic
Skov, Robert L.
Larsen, Anders R.
Andersen, Paal S.
Price, Lance B.
dc.contributor.researchgroup.spa.fl_str_mv Microbiología Molecular
dc.subject.decs.none.fl_str_mv Staphylococcus aureus
Filogenia
Phylogeny
topic Staphylococcus aureus
Filogenia
Phylogeny
Polimorfismo genético
Genetic polymorphism
Polimorfismo de un solo nucleótido
Single nucleotide polymorphism
http://aims.fao.org/aos/agrovoc/c_24031
http://aims.fao.org/aos/agrovoc/c_0273c413
dc.subject.agrovoc.none.fl_str_mv Polimorfismo genético
Genetic polymorphism
Polimorfismo de un solo nucleótido
Single nucleotide polymorphism
dc.subject.agrovocuri.none.fl_str_mv http://aims.fao.org/aos/agrovoc/c_24031
http://aims.fao.org/aos/agrovoc/c_0273c413
description ABSTRACT: Staphylococcus aureus clonal complex 398 (CC398) isolates cluster into two distinct phylogenetic clades based on singlenucleotide polymorphisms (SNPs) revealing a basal human clade and a more derived livestock clade. The scn and tet(M) genes are strongly associated with the human and the livestock clade, respectively, due to loss and acquisition of mobile genetic elements. We present canonical single-nucleotide polymorphism (canSNP) assays that differentiate the two major host-associated S. aureus CC398 clades and a duplex PCR assay for detection of scn and tet(M). The canSNP assays correctly placed 88 S. aureus CC398 isolates from a reference collection into the human and livestock clades and the duplex PCR assay correctly identified scn and tet(M). The assays were successfully applied to a geographically diverse collection of 272 human S. aureus CC398 isolates. The simple assays described here generate signals comparable to a whole-genome phylogeny for major clade assignment and are easily integrated into S. aureus CC398 surveillance programs and epidemiological studies.
publishDate 2013
dc.date.issued.none.fl_str_mv 2013
dc.date.accessioned.none.fl_str_mv 2021-09-03T17:23:46Z
dc.date.available.none.fl_str_mv 2021-09-03T17:23:46Z
dc.type.spa.fl_str_mv Artículo de investigación
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
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dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/22136
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0079645
dc.identifier.eissn.none.fl_str_mv 1932-6203
url http://hdl.handle.net/10495/22136
identifier_str_mv 10.1371/journal.pone.0079645
1932-6203
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv PLoS ONE.
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dc.relation.citationissue.spa.fl_str_mv 11
dc.relation.citationstartpage.spa.fl_str_mv 1
dc.relation.citationvolume.spa.fl_str_mv 8
dc.relation.ispartofjournal.spa.fl_str_mv PLoS ONE
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/2.5/co/
dc.rights.uri.spa.fl_str_mv https://creativecommons.org/licenses/by/4.0/
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dc.publisher.place.spa.fl_str_mv San Francisco, Estados Unidos
institution Universidad de Antioquia
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spelling Stegger, MarcLiu, Cindy M.Larsen, JesperSoldanova, KaterinaAziz, MalihaContente Cuomo, TaniaPetersen, AndreasVandendriessche, StienJiménez Quiceno, Judy NataliaMammina, CaterinaBelkum, Alex VanSalmenlinna, SaaraLaurent, FredericSkov, Robert L.Larsen, Anders R.Andersen, Paal S.Price, Lance B.Microbiología Molecular2021-09-03T17:23:46Z2021-09-03T17:23:46Z2013http://hdl.handle.net/10495/2213610.1371/journal.pone.00796451932-6203ABSTRACT: Staphylococcus aureus clonal complex 398 (CC398) isolates cluster into two distinct phylogenetic clades based on singlenucleotide polymorphisms (SNPs) revealing a basal human clade and a more derived livestock clade. The scn and tet(M) genes are strongly associated with the human and the livestock clade, respectively, due to loss and acquisition of mobile genetic elements. We present canonical single-nucleotide polymorphism (canSNP) assays that differentiate the two major host-associated S. aureus CC398 clades and a duplex PCR assay for detection of scn and tet(M). The canSNP assays correctly placed 88 S. aureus CC398 isolates from a reference collection into the human and livestock clades and the duplex PCR assay correctly identified scn and tet(M). The assays were successfully applied to a geographically diverse collection of 272 human S. aureus CC398 isolates. The simple assays described here generate signals comparable to a whole-genome phylogeny for major clade assignment and are easily integrated into S. aureus CC398 surveillance programs and epidemiological studies.COL00137465application/pdfengPublic Library of ScienceSan Francisco, Estados Unidoshttp://creativecommons.org/licenses/by/2.5/co/https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Rapid differentiation between Livestock-Associated and Livestock-Independent Staphylococcus aureus CC398 CladesArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionStaphylococcus aureusFilogeniaPhylogenyPolimorfismo genéticoGenetic polymorphismPolimorfismo de un solo nucleótidoSingle nucleotide polymorphismhttp://aims.fao.org/aos/agrovoc/c_24031http://aims.fao.org/aos/agrovoc/c_0273c413PLoS ONE.51118PLoS ONEPublicationORIGINALSoldanovaKaterina_2013_DifferentiationLivestock.pdfSoldanovaKaterina_2013_DifferentiationLivestock.pdfArtículo de investigaciónapplication/pdf623893https://bibliotecadigital.udea.edu.co/bitstreams/38e249e5-2b39-46fb-ba38-4e9b88721686/downloada5f47e52b21f657a315636d86be412a1MD51trueAnonymousREADCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927https://bibliotecadigital.udea.edu.co/bitstreams/37487786-149b-4041-a665-c5f9553978ff/download1646d1f6b96dbbbc38035efc9239ac9cMD52falseAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/77a980b4-79db-4849-9128-1761ea01a86e/download8a4605be74aa9ea9d79846c1fba20a33MD53falseAnonymousREADTEXTSoldanovaKaterina_2013_DifferentiationLivestock.pdf.txtSoldanovaKaterina_2013_DifferentiationLivestock.pdf.txtExtracted texttext/plain27019https://bibliotecadigital.udea.edu.co/bitstreams/31e72deb-00aa-46a1-95e3-9ed78da8ef18/download520abf1b4ef9e0c26f1affdc620a31d1MD54falseAnonymousREADTHUMBNAILSoldanovaKaterina_2013_DifferentiationLivestock.pdf.jpgSoldanovaKaterina_2013_DifferentiationLivestock.pdf.jpgGenerated Thumbnailimage/jpeg17640https://bibliotecadigital.udea.edu.co/bitstreams/b9a1326e-7c02-4f44-aa4d-4d218040fa4d/downloadb76f9b7dcb16b96ea6fb3f552fc9771dMD55falseAnonymousREAD10495/22136oai:bibliotecadigital.udea.edu.co:10495/221362025-03-27 01:21:48.665http://creativecommons.org/licenses/by/2.5/co/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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