Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango Pulp
ABSTRACT: Previous studies have indicated that mango fruit has a chemopreventive capacity against colorectal cancer cells. The objective of this research was to evaluate the effect of an aqueous extract of lyophilized mango pulp (LMPE) on colon adenocarcinoma cells (SW480) and their metastatic deriv...
- Autores:
-
Lozano Casabianca, Gustavo Argenor
Maldonado Celis, María Elena
Arango Varela, Sandra Sulay
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2023
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/41248
- Acceso en línea:
- https://hdl.handle.net/10495/41248
- Palabra clave:
- Adenocarcinoma - patología
Adenocarcinoma - pathology
Apoptosis
Proteínas Reguladoras de la Apoptosis
Apoptosis Regulatory Proteins
Autofagia
Autophagy
Línea Celular Tumoral
Cell Line, Tumor
Neoplasias del Colon
Colonic Neoplasms
Mangifera
Metaloproteinasas de la Matriz
Matrix Metalloproteinases
https://id.nlm.nih.gov/mesh/D000230
https://id.nlm.nih.gov/mesh/D017209
https://id.nlm.nih.gov/mesh/D051017
https://id.nlm.nih.gov/mesh/D001343
https://id.nlm.nih.gov/mesh/D045744
https://id.nlm.nih.gov/mesh/D003110
https://id.nlm.nih.gov/mesh/D031022
https://id.nlm.nih.gov/mesh/D020782
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by/4.0/
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| dc.title.spa.fl_str_mv |
Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango Pulp |
| title |
Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango Pulp |
| spellingShingle |
Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango Pulp Adenocarcinoma - patología Adenocarcinoma - pathology Apoptosis Proteínas Reguladoras de la Apoptosis Apoptosis Regulatory Proteins Autofagia Autophagy Línea Celular Tumoral Cell Line, Tumor Neoplasias del Colon Colonic Neoplasms Mangifera Metaloproteinasas de la Matriz Matrix Metalloproteinases https://id.nlm.nih.gov/mesh/D000230 https://id.nlm.nih.gov/mesh/D017209 https://id.nlm.nih.gov/mesh/D051017 https://id.nlm.nih.gov/mesh/D001343 https://id.nlm.nih.gov/mesh/D045744 https://id.nlm.nih.gov/mesh/D003110 https://id.nlm.nih.gov/mesh/D031022 https://id.nlm.nih.gov/mesh/D020782 |
| title_short |
Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango Pulp |
| title_full |
Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango Pulp |
| title_fullStr |
Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango Pulp |
| title_full_unstemmed |
Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango Pulp |
| title_sort |
Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango Pulp |
| dc.creator.fl_str_mv |
Lozano Casabianca, Gustavo Argenor Maldonado Celis, María Elena Arango Varela, Sandra Sulay |
| dc.contributor.author.none.fl_str_mv |
Lozano Casabianca, Gustavo Argenor Maldonado Celis, María Elena Arango Varela, Sandra Sulay |
| dc.contributor.researchgroup.spa.fl_str_mv |
Impacto de Componentes Alimentarios en la Salud |
| dc.subject.decs.none.fl_str_mv |
Adenocarcinoma - patología Adenocarcinoma - pathology Apoptosis Proteínas Reguladoras de la Apoptosis Apoptosis Regulatory Proteins Autofagia Autophagy Línea Celular Tumoral Cell Line, Tumor Neoplasias del Colon Colonic Neoplasms Mangifera Metaloproteinasas de la Matriz Matrix Metalloproteinases |
| topic |
Adenocarcinoma - patología Adenocarcinoma - pathology Apoptosis Proteínas Reguladoras de la Apoptosis Apoptosis Regulatory Proteins Autofagia Autophagy Línea Celular Tumoral Cell Line, Tumor Neoplasias del Colon Colonic Neoplasms Mangifera Metaloproteinasas de la Matriz Matrix Metalloproteinases https://id.nlm.nih.gov/mesh/D000230 https://id.nlm.nih.gov/mesh/D017209 https://id.nlm.nih.gov/mesh/D051017 https://id.nlm.nih.gov/mesh/D001343 https://id.nlm.nih.gov/mesh/D045744 https://id.nlm.nih.gov/mesh/D003110 https://id.nlm.nih.gov/mesh/D031022 https://id.nlm.nih.gov/mesh/D020782 |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D000230 https://id.nlm.nih.gov/mesh/D017209 https://id.nlm.nih.gov/mesh/D051017 https://id.nlm.nih.gov/mesh/D001343 https://id.nlm.nih.gov/mesh/D045744 https://id.nlm.nih.gov/mesh/D003110 https://id.nlm.nih.gov/mesh/D031022 https://id.nlm.nih.gov/mesh/D020782 |
| description |
ABSTRACT: Previous studies have indicated that mango fruit has a chemopreventive capacity against colorectal cancer cells. The objective of this research was to evaluate the effect of an aqueous extract of lyophilized mango pulp (LMPE) on colon adenocarcinoma cells (SW480) and their metastatic derivatives (SW620) death and cellular invasion. DNA fragmentation was assessed by TUNEL assay; autophagy and expression of DR4 and Bcl-2 by flow cytometry; the expression of 35 apoptosis-related proteins and of matrix metalloproteinases 7 and 9 by immunodetection; and the invasive capacity of the cells by Boyden chamber. The results showed that LMPE at 30 mg/mL and 48 h of exposure results in DNA fragmentation and apoptosis in SW480 (p < 0.001) and SW620 (p < 0.01) cells. Additionally, LMPE decreased autophagy in the SW480 and SW620 cell lines (p < 0.001), which could sensitize them to the DNA damage generated by LMPE. The LMPE did not modulate the expression of matrix metalloproteinases 7 and 9, nor did it affect cellular invasion processes in the SW480 and SW620 cell lines. In conclusion, LMPE induces apoptosis and decreases autophagy in SW480 and SW620 cells. |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2024-08-20T20:25:06Z |
| dc.date.available.none.fl_str_mv |
2024-08-20T20:25:06Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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Lozano-Casabianca, G.A.; Arango-Varela, S.S.; Maldonado- Celis, M.E. Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango Pulp. Int. J. Environ. Res. Public Health 2023, 20, 4165. https://doi.org/10.3390/ ijerph20054165 |
| dc.identifier.issn.none.fl_str_mv |
1661-7827 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/41248 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/ijerph20054165 |
| dc.identifier.eissn.none.fl_str_mv |
1660-4601 |
| identifier_str_mv |
Lozano-Casabianca, G.A.; Arango-Varela, S.S.; Maldonado- Celis, M.E. Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango Pulp. Int. J. Environ. Res. Public Health 2023, 20, 4165. https://doi.org/10.3390/ ijerph20054165 1661-7827 10.3390/ijerph20054165 1660-4601 |
| url |
https://hdl.handle.net/10495/41248 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Int. J. Environ. Res. Public. Health. |
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16 |
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5 |
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1 |
| dc.relation.citationvolume.spa.fl_str_mv |
20 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
International Journal of Environmental Research and Public Health |
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openAccess |
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16 páginas |
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MDPI |
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Lozano Casabianca, Gustavo ArgenorMaldonado Celis, María ElenaArango Varela, Sandra SulayImpacto de Componentes Alimentarios en la Salud2024-08-20T20:25:06Z2024-08-20T20:25:06Z2023Lozano-Casabianca, G.A.; Arango-Varela, S.S.; Maldonado- Celis, M.E. Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango Pulp. Int. J. Environ. Res. Public Health 2023, 20, 4165. https://doi.org/10.3390/ ijerph200541651661-7827https://hdl.handle.net/10495/4124810.3390/ijerph200541651660-4601ABSTRACT: Previous studies have indicated that mango fruit has a chemopreventive capacity against colorectal cancer cells. The objective of this research was to evaluate the effect of an aqueous extract of lyophilized mango pulp (LMPE) on colon adenocarcinoma cells (SW480) and their metastatic derivatives (SW620) death and cellular invasion. DNA fragmentation was assessed by TUNEL assay; autophagy and expression of DR4 and Bcl-2 by flow cytometry; the expression of 35 apoptosis-related proteins and of matrix metalloproteinases 7 and 9 by immunodetection; and the invasive capacity of the cells by Boyden chamber. The results showed that LMPE at 30 mg/mL and 48 h of exposure results in DNA fragmentation and apoptosis in SW480 (p < 0.001) and SW620 (p < 0.01) cells. Additionally, LMPE decreased autophagy in the SW480 and SW620 cell lines (p < 0.001), which could sensitize them to the DNA damage generated by LMPE. The LMPE did not modulate the expression of matrix metalloproteinases 7 and 9, nor did it affect cellular invasion processes in the SW480 and SW620 cell lines. In conclusion, LMPE induces apoptosis and decreases autophagy in SW480 and SW620 cells.Colombia. Ministerio de Ciencia, Tecnología e Innovación - MincienciasColombia. Ministerio de Educación Nacional - MineducaciónColombia. Ministerio de Comercio, Industria, Industria y TurismoICETEXCOL008381116 páginasapplication/pdfengMDPIBasilea, Suizahttps://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/2.5/co/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango PulpArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAdenocarcinoma - patologíaAdenocarcinoma - pathologyApoptosisProteínas Reguladoras de la ApoptosisApoptosis Regulatory ProteinsAutofagiaAutophagyLínea Celular TumoralCell Line, TumorNeoplasias del ColonColonic NeoplasmsMangiferaMetaloproteinasas de la MatrizMatrix Metalloproteinaseshttps://id.nlm.nih.gov/mesh/D000230https://id.nlm.nih.gov/mesh/D017209https://id.nlm.nih.gov/mesh/D051017https://id.nlm.nih.gov/mesh/D001343https://id.nlm.nih.gov/mesh/D045744https://id.nlm.nih.gov/mesh/D003110https://id.nlm.nih.gov/mesh/D031022https://id.nlm.nih.gov/mesh/D020782Int. J. Environ. Res. Public. 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