Genetic diversity of Leptospira in northwestern Colombia: first report of Leptospira santarosai as a recognised leptospirosis agent

ABSTRACT: The region of Antioquia in northeastern Colombia has the highest number of reported leptospirosis cases in the country. It also shows high seroprevalence indexes in the general population and socio-environmental conditions favourable for the transmission of the disease between humans and animals. In this study, 25 Leptospira isolates from Colombia’s Antioquia department were identified to the species level as L. santarosai (12), L. interrogans (9) and L. meyeri (4) using phylogenetic analysis of the Amidohydrolase gene. Typing at the serovar level was performed using multilocus sequence typing (MLST) and monoclonal antibodies. The serovars Canalzonae, Babudieri, Alice, Beye, and Copenhageni have been identified as causing human or animal infections in Antioquia, Colombia. The four environmental isolates were not identified to the serovar level. L. santarosai serovar Canalzonae and Alice were identified as new etiologic agents of human leptospirosis in Antioquia, Colombia. This paper reports species and serovars that were previously unknown in the region.

• Autores: López Quintero, Juan Álvaro; Peláez Sánchez, Ronald Guillermo; Pereira, Martha María; Arboleda Naranjo, Margarita; Agudelo Flórez, Piedad
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2016
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/38752
• Acceso en línea: https://hdl.handle.net/10495/38752
• Palabra clave: Leptospirosis; Leptospira; Colombia; Tipificación de Secuencias Multilocus; Multilocus Sequence Typing; Técnicas de Tipificación Bacteriana; Bacterial Typing Techniques; Electroforesis en Gel de Campo Pulsado; Electrophoresis, Gel, Pulsed-Field; Variación Genética; Genetic Variation; ARN Ribosómico 16S; RNA, Ribosomal, 16S; https://id.nlm.nih.gov/mesh/D007922; https://id.nlm.nih.gov/mesh/D007919; https://id.nlm.nih.gov/mesh/D003105; https://id.nlm.nih.gov/mesh/D058885; https://id.nlm.nih.gov/mesh/D015373; https://id.nlm.nih.gov/mesh/D016521; https://id.nlm.nih.gov/mesh/D014644; https://id.nlm.nih.gov/mesh/D012336
• Rights: openAccess
• License: https://creativecommons.org/licenses/by/4.0/