Validation of a Liquid Chromatographic Method for Determination of Sulphadoxine and Pyrimethamine in Whole Blood Spotted on Filter Paper
ABSTRACT: Objective: to validate an analytical method for simultaneous determination and quantification of sulphadoxine and pyrimethamine in human blood dried onto filter paper, whose cost and analysis time can be reduced. Methods: whole blood spotted on filter paper of a healthy volunteer and solut...
- Autores:
-
Márquez Fernández, Diana Margarita
Pabón Vidal, Adriana Lucía
López Córdoba, Carlos Alberto
Blair Trujillo, Silvia
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2012
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/35097
- Acceso en línea:
- https://hdl.handle.net/10495/35097
- Palabra clave:
- Pyrimethamine
Pirimetamina
Sulfadoxine
Sulfadoxina
Validation Study
Estudio de Validación
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc/4.0/
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| dc.title.spa.fl_str_mv |
Validation of a Liquid Chromatographic Method for Determination of Sulphadoxine and Pyrimethamine in Whole Blood Spotted on Filter Paper |
| dc.title.translated.spa.fl_str_mv |
Validación de un método de cromatografía líquida para determinar la presencia de sulfadoxina y pirimetamina en muestra de sangre secada sobre papel de filtro |
| title |
Validation of a Liquid Chromatographic Method for Determination of Sulphadoxine and Pyrimethamine in Whole Blood Spotted on Filter Paper |
| spellingShingle |
Validation of a Liquid Chromatographic Method for Determination of Sulphadoxine and Pyrimethamine in Whole Blood Spotted on Filter Paper Pyrimethamine Pirimetamina Sulfadoxine Sulfadoxina Validation Study Estudio de Validación |
| title_short |
Validation of a Liquid Chromatographic Method for Determination of Sulphadoxine and Pyrimethamine in Whole Blood Spotted on Filter Paper |
| title_full |
Validation of a Liquid Chromatographic Method for Determination of Sulphadoxine and Pyrimethamine in Whole Blood Spotted on Filter Paper |
| title_fullStr |
Validation of a Liquid Chromatographic Method for Determination of Sulphadoxine and Pyrimethamine in Whole Blood Spotted on Filter Paper |
| title_full_unstemmed |
Validation of a Liquid Chromatographic Method for Determination of Sulphadoxine and Pyrimethamine in Whole Blood Spotted on Filter Paper |
| title_sort |
Validation of a Liquid Chromatographic Method for Determination of Sulphadoxine and Pyrimethamine in Whole Blood Spotted on Filter Paper |
| dc.creator.fl_str_mv |
Márquez Fernández, Diana Margarita Pabón Vidal, Adriana Lucía López Córdoba, Carlos Alberto Blair Trujillo, Silvia |
| dc.contributor.author.none.fl_str_mv |
Márquez Fernández, Diana Margarita Pabón Vidal, Adriana Lucía López Córdoba, Carlos Alberto Blair Trujillo, Silvia |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo Malaria Productos Naturales Marinos |
| dc.subject.decs.none.fl_str_mv |
Pyrimethamine Pirimetamina Sulfadoxine Sulfadoxina Validation Study Estudio de Validación |
| topic |
Pyrimethamine Pirimetamina Sulfadoxine Sulfadoxina Validation Study Estudio de Validación |
| description |
ABSTRACT: Objective: to validate an analytical method for simultaneous determination and quantification of sulphadoxine and pyrimethamine in human blood dried onto filter paper, whose cost and analysis time can be reduced. Methods: whole blood spotted on filter paper of a healthy volunteer and solutions of sulphadoxine-pyrimethamine standard mixture were used. HPLC separations were carried out on Agilent equipment using a LiChrospher® column C18 with a mobile phase acetonitrile/0.1 M potassium phosphate buffer at pH 3.0 (1:1) for eight minutes under isocratic conditions. A flow rate of 0.7 mL/min, and a 20 mL volume injection were used. External standard method for quantitation of analytes was used. Results: the HPLC method described for the simultaneous determination of sulphadoxine and pyrimethamine in 100 mL of whole blood spotted on filter paper has been found to be linear, precise, accurate and selective. In this method, the sample preparation is simple using liquid liquid extraction, and HPLC with ultraviolet detection is used. Conclusions: a simple, fast and sensitive method for determination of sulphadoxine and pyrimethamine in human blood dried onto filter paper was validated. This method can be used for the monitoring of both metabolites in pharmacokinetic and clinical studies. |
| publishDate |
2012 |
| dc.date.issued.none.fl_str_mv |
2012 |
| dc.date.accessioned.none.fl_str_mv |
2023-05-24T19:45:42Z |
| dc.date.available.none.fl_str_mv |
2023-05-24T19:45:42Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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0034-7515 |
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https://hdl.handle.net/10495/35097 |
| dc.identifier.eissn.none.fl_str_mv |
1561-2988 |
| identifier_str_mv |
0034-7515 1561-2988 |
| url |
https://hdl.handle.net/10495/35097 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Cuba. Farm. |
| dc.relation.citationendpage.spa.fl_str_mv |
319 |
| dc.relation.citationissue.spa.fl_str_mv |
3 |
| dc.relation.citationstartpage.spa.fl_str_mv |
311 |
| dc.relation.citationvolume.spa.fl_str_mv |
46 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Revista Cubana de Farmacia |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0/ |
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http://creativecommons.org/licenses/by-nc/2.5/co/ |
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openAccess |
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9 páginas |
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application/pdf |
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Centro Nacional de Información de Ciencias Médicas |
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La Habana, Cuba |
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Universidad de Antioquia |
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Márquez Fernández, Diana MargaritaPabón Vidal, Adriana LucíaLópez Córdoba, Carlos AlbertoBlair Trujillo, SilviaGrupo MalariaProductos Naturales Marinos2023-05-24T19:45:42Z2023-05-24T19:45:42Z20120034-7515https://hdl.handle.net/10495/350971561-2988ABSTRACT: Objective: to validate an analytical method for simultaneous determination and quantification of sulphadoxine and pyrimethamine in human blood dried onto filter paper, whose cost and analysis time can be reduced. Methods: whole blood spotted on filter paper of a healthy volunteer and solutions of sulphadoxine-pyrimethamine standard mixture were used. HPLC separations were carried out on Agilent equipment using a LiChrospher® column C18 with a mobile phase acetonitrile/0.1 M potassium phosphate buffer at pH 3.0 (1:1) for eight minutes under isocratic conditions. A flow rate of 0.7 mL/min, and a 20 mL volume injection were used. External standard method for quantitation of analytes was used. Results: the HPLC method described for the simultaneous determination of sulphadoxine and pyrimethamine in 100 mL of whole blood spotted on filter paper has been found to be linear, precise, accurate and selective. In this method, the sample preparation is simple using liquid liquid extraction, and HPLC with ultraviolet detection is used. Conclusions: a simple, fast and sensitive method for determination of sulphadoxine and pyrimethamine in human blood dried onto filter paper was validated. This method can be used for the monitoring of both metabolites in pharmacokinetic and clinical studies.RESUMEN: Objetivo: validar un método de análisis para la determinación y cuantificación simultánea de sulfadoxina y pirimetamina en sangre humana secada sobre papel de filtro que sea rápido y barato. Métodos: se usó sangre de un voluntario sano impregnada sobre papel de filtro y soluciones estándar de la mezcla sulfadoxina y pririmetamina. Las separaciones por cromatografía líquida de alta resolución (CLAR) se hicieron en un equipo Agilent sobre una columna C18 LiChrospher® con acetonitrilo/buffer fosfato de potasio 0,1 M a pH 3,0 como fase móvil, usando condiciones isocráticas durante 8 min. Se usó un flujo de 0,7 mL/min y un volumen de inyección de 20 mL. Para la cuantificación de los analitos se utilizó el método del estándar externo. Resultados: el método CLAR descrito para la determinación simultánea de sulfadoxina y pirimetamina en 100 mL de sangre impregnada sobre el papel de filtro mostró linealidad, precisión, exactitud y selectividad. En este método la preparación de la muestra es simple ya que usa extracción líquido-líquido y detección ultravioleta. Conclusión: se obtuvo un método validado que es simple, rápido y sensible para la determinación de sulfadoxina y pirimetamina en sangre humana impregnada sobre papel de filtro, que puede ser usado para el monitoreo de ambos metabolitos en estudios farmacocinéticos y clínicos.COL0007524COL00150439 páginasapplication/pdfengCentro Nacional de Información de Ciencias MédicasLa Habana, Cubahttps://creativecommons.org/licenses/by-nc/4.0/http://creativecommons.org/licenses/by-nc/2.5/co/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Validation of a Liquid Chromatographic Method for Determination of Sulphadoxine and Pyrimethamine in Whole Blood Spotted on Filter PaperValidación de un método de cromatografía líquida para determinar la presencia de sulfadoxina y pirimetamina en muestra de sangre secada sobre papel de filtroArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPyrimethaminePirimetaminaSulfadoxineSulfadoxinaValidation StudyEstudio de ValidaciónRev. Cuba. 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