Analysis of high molecular mass compounds from the spider pamphobeteus verdolaga venom gland. A transcriptomic and MS ID approach

ABSTRACT: Nowadays, spider venom research focuses on the neurotoxic activity of small peptides. In this study, we investigated high-molecular-mass compounds that have either enzymatic activity or housekeeping functions present in either the venom gland or venom of Pamphobeteus verdolaga. We used proteomic and transcriptomic-assisted approaches to recognize the proteins sequences related to high-molecular-mass compounds present in either venom gland or venom. We report the amino acid sequences (partial or complete) of 45 high-molecular-mass compounds detected by transcriptomics showing similarity to other proteins with either enzymatic activity (i.e., phospholipases A2, kunitz-type, hyaluronidases, and sphingomyelinase D) or housekeeping functions involved in the signaling process, glucanotransferase function, and beta-N-acetylglucosaminidase activity. MS/MS analysis showed fragments exhibiting a resemblance similarity with different sequences detected by transcriptomics corresponding to sphingomyelinase D, hyaluronidase, lycotoxins, cysteine-rich secretory proteins, and kunitz-type serine protease inhibitors, among others. Additionally, we report a probably new protein sequence corresponding to the lycotoxin family detected by transcriptomics. The phylogeny analysis suggested that P. verdolaga includes a basal protein that underwent a duplication event that gave origin to the lycotoxin proteins reported for Lycosa sp. This approach allows proposing an evolutionary relationship of high-molecular-mass proteins among P. verdolaga and other spider species.

• Autores: Estrada Gómez, Sebastián; Segura Latorre, Cesar; Arenas Gómez, Claudia Marcela; Vargas Muñoz, Leidy Johana; Saldarriaga Córdoba, Monica María
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2021
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/41071
• Acceso en línea: https://hdl.handle.net/10495/41071
• Palabra clave: Secuencia de Aminoácidos; Amino Acid Sequence; Proteínas de Artrópodos - análisis; Arthropod Proteins - analysis; Proteínas de Artrópodos - química; Arthropod Proteins - chemistry; Glándulas Exocrinas - química; Exocrine Glands - chemistry; Peso Molecular; Molecular Weight; Proteoma; Proteome; Venenos de Araña; Spider Venoms; Arañas; Spiders; Espectrometría de Masas en Tándem; Tandem Mass Spectrometry; Transcriptoma; Transcriptome; https://id.nlm.nih.gov/mesh/D000595; https://id.nlm.nih.gov/mesh/D060829; https://id.nlm.nih.gov/mesh/D005088; https://id.nlm.nih.gov/mesh/D008970; https://id.nlm.nih.gov/mesh/D020543; https://id.nlm.nih.gov/mesh/D013111; https://id.nlm.nih.gov/mesh/D013112; https://id.nlm.nih.gov/mesh/D053719; https://id.nlm.nih.gov/mesh/D059467
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/2.5/co/