C. saccharoperbutylacetonicum N1-4 electroactivity and co2 fixation under different electrochemical conditions

The capability of certain microbial strains to uptake electrons and fix CO2 can be exploited to capture greenhouse gas and convert it into products of interest through a process called microbial bioelectrosynthesis. This study evaluated the capability of C. saccharoperbutylacetonicum N1-4 to utilize bicarbonate for growth, by both supplying electrons in a single-cell reactor and using a graphite-felt assembly as electrodes. The medium was supplemented with 4 g/L bicarbonate and 200 μM NADH. An open circuit experiment was carried out in a medium with bicarbonate and no complex nitrogen sources. The applied potential was -600 mVAg/AgCl. The impedance spectroscopy and cyclic voltammetry techniques were used to characterize and monitor the reduction and oxidation peaks. The conditions that promote the highest observed specific growth rate (0.87±0.18 h-1), were -600 mV, 4 g/L HCO3 -, and NADH. The growth rates of 0.57±0.01 h-1 were observed at 4 g/L HCO3 - without any potential input, and 0.51±0.10 h-1 at a potential of -600 mV in the presence of NADH. The results showed that an environment that provides exogenous electrons and an externally applied potential promotes the capability of C. saccharoperbutylacetonicum N1-4 to reduce CO2, as evidenced when biomass concentration and specific growth rate were increased.

• Autores: Quintero Díaz, Juan Carlos; García Mogollón, Carlos Alberto; Avignone Rossa, Claudio; Arrieta Almario, Álvaro
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2023
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/45926
• Acceso en línea: https://hdl.handle.net/10495/45926
• Palabra clave: Síntesis microbiológica; Microbiological synthesis; Dióxido de Carbono; Carbon Dioxide; NAD; Gases de Efecto Invernadero; Greenhouse Gases; Clostridium; Análisis Espectral; Spectrum Analysis; Biomasa; Biomass; Clostridium saccharoperbutylacetonicum; http://id.loc.gov/authorities/subjects/sh85084780; https://id.nlm.nih.gov/mesh/D002245; https://id.nlm.nih.gov/mesh/D009243; https://id.nlm.nih.gov/mesh/D000074382; https://id.nlm.nih.gov/mesh/D003013; https://id.nlm.nih.gov/mesh/D013057; https://id.nlm.nih.gov/mesh/D018533; ODS 7: Energía asequible y no contaminante. Garantizar el acceso a una energía asequible, fiable, sostenible y moderna para todos; ODS 12: Producción y consumo responsables. Garantizar modalidades de consumo y producción sostenibles
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/4.0/