Licania arborea fraction bioactive potential assessment in jurkat and cho-k1 cell lines
ABSTRACT: Introduction: Several species of Chrysobalanaceae plants have shown a variety of biological activities, exhibiting a source of interesting compounds from the pharmacological point of view. Objective: To assess the bioactive potential of L. arborea fractions on Jurkat and CHO-K1 cell lines....
- Autores:
-
Márquez Fernández, Diana Margarita
Estrada Ortiz, Natalia
Márquez Fernández, María Elena
López Ortiz, Juan Bautista
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/38811
- Acceso en línea:
- https://hdl.handle.net/10495/38811
- Palabra clave:
- Genotoxicidad
Genotoxicity
Línea Celular
Cell Line
Citotoxicidad
Cytotoxicity
http://aims.fao.org/aos/agrovoc/c_34251
https://id.nlm.nih.gov/mesh/D002460
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc/2.5/co/
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| dc.title.spa.fl_str_mv |
Licania arborea fraction bioactive potential assessment in jurkat and cho-k1 cell lines |
| dc.title.translated.spa.fl_str_mv |
Valoración del potencial bioactivo de la fracción de Licania arbórea en las líneas celulares Jurkat y Cho-K1 |
| title |
Licania arborea fraction bioactive potential assessment in jurkat and cho-k1 cell lines |
| spellingShingle |
Licania arborea fraction bioactive potential assessment in jurkat and cho-k1 cell lines Genotoxicidad Genotoxicity Línea Celular Cell Line Citotoxicidad Cytotoxicity http://aims.fao.org/aos/agrovoc/c_34251 https://id.nlm.nih.gov/mesh/D002460 |
| title_short |
Licania arborea fraction bioactive potential assessment in jurkat and cho-k1 cell lines |
| title_full |
Licania arborea fraction bioactive potential assessment in jurkat and cho-k1 cell lines |
| title_fullStr |
Licania arborea fraction bioactive potential assessment in jurkat and cho-k1 cell lines |
| title_full_unstemmed |
Licania arborea fraction bioactive potential assessment in jurkat and cho-k1 cell lines |
| title_sort |
Licania arborea fraction bioactive potential assessment in jurkat and cho-k1 cell lines |
| dc.creator.fl_str_mv |
Márquez Fernández, Diana Margarita Estrada Ortiz, Natalia Márquez Fernández, María Elena López Ortiz, Juan Bautista |
| dc.contributor.author.none.fl_str_mv |
Márquez Fernández, Diana Margarita Estrada Ortiz, Natalia Márquez Fernández, María Elena López Ortiz, Juan Bautista |
| dc.contributor.researchgroup.spa.fl_str_mv |
Productos Naturales Marinos |
| dc.subject.decs.none.fl_str_mv |
Genotoxicidad Genotoxicity Línea Celular Cell Line |
| topic |
Genotoxicidad Genotoxicity Línea Celular Cell Line Citotoxicidad Cytotoxicity http://aims.fao.org/aos/agrovoc/c_34251 https://id.nlm.nih.gov/mesh/D002460 |
| dc.subject.agrovoc.none.fl_str_mv |
Citotoxicidad Cytotoxicity |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_34251 |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D002460 |
| description |
ABSTRACT: Introduction: Several species of Chrysobalanaceae plants have shown a variety of biological activities, exhibiting a source of interesting compounds from the pharmacological point of view. Objective: To assess the bioactive potential of L. arborea fractions on Jurkat and CHO-K1 cell lines. Methods: All the experiments were carried out in Jurkat and CHO-K1 (mammalian cell lines). Cytotoxicity of the fractions was assessed via tTrypan blue dye and tetrazolium salt (MTT) assays. Genotoxicity was evaluated determining the sister chromatid exchange (SCE). Antiproliferative effect was estimated by clonogenic assay and cell cycle progression. Furthermore, proliferative kinetics were assessed by SCE of L. arborea fractions. Results: The IC50 of F8 and F10 fractions was approximately 100 µg/mL for Jurkat and CHO-K1 cell lines. Cytotoxicity of the evaluated fractions led to reduced cell viability and cloning capability. Particularly, fraction F8 showed genotoxic effect reflected in increments of SCEs frequency, mainly on Jurkat cells. Conclusions: Jurkat and CHO-K1 cells clearly displayed a dose dependent cytotoxicity and genotoxicity, although the effect was more severe on the lymphoid cell line compared with the noncancerous cells, showing total absence of mitosis in the Jurkat cell line treated with fraction F8 (100μg/mL). |
| publishDate |
2016 |
| dc.date.issued.none.fl_str_mv |
2016 |
| dc.date.accessioned.none.fl_str_mv |
2024-03-27T15:24:24Z |
| dc.date.available.none.fl_str_mv |
2024-03-27T15:24:24Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.issn.none.fl_str_mv |
0034-7515 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/38811 |
| dc.identifier.eissn.none.fl_str_mv |
1561-2988 |
| identifier_str_mv |
0034-7515 1561-2988 |
| url |
https://hdl.handle.net/10495/38811 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Cuba. Farm. |
| dc.relation.citationendpage.spa.fl_str_mv |
66 |
| dc.relation.citationissue.spa.fl_str_mv |
4 |
| dc.relation.citationstartpage.spa.fl_str_mv |
62 |
| dc.relation.citationvolume.spa.fl_str_mv |
50 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Revista Cubana de Farmacia |
| dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc/2.5/co/ |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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openAccess |
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12 páginas |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Centro Nacional de Información de Ciencias Médicas |
| dc.publisher.place.spa.fl_str_mv |
La Habana, Cuba |
| institution |
Universidad de Antioquia |
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Márquez Fernández, Diana MargaritaEstrada Ortiz, NataliaMárquez Fernández, María ElenaLópez Ortiz, Juan BautistaProductos Naturales Marinos2024-03-27T15:24:24Z2024-03-27T15:24:24Z20160034-7515https://hdl.handle.net/10495/388111561-2988ABSTRACT: Introduction: Several species of Chrysobalanaceae plants have shown a variety of biological activities, exhibiting a source of interesting compounds from the pharmacological point of view. Objective: To assess the bioactive potential of L. arborea fractions on Jurkat and CHO-K1 cell lines. Methods: All the experiments were carried out in Jurkat and CHO-K1 (mammalian cell lines). Cytotoxicity of the fractions was assessed via tTrypan blue dye and tetrazolium salt (MTT) assays. Genotoxicity was evaluated determining the sister chromatid exchange (SCE). Antiproliferative effect was estimated by clonogenic assay and cell cycle progression. Furthermore, proliferative kinetics were assessed by SCE of L. arborea fractions. Results: The IC50 of F8 and F10 fractions was approximately 100 µg/mL for Jurkat and CHO-K1 cell lines. Cytotoxicity of the evaluated fractions led to reduced cell viability and cloning capability. Particularly, fraction F8 showed genotoxic effect reflected in increments of SCEs frequency, mainly on Jurkat cells. Conclusions: Jurkat and CHO-K1 cells clearly displayed a dose dependent cytotoxicity and genotoxicity, although the effect was more severe on the lymphoid cell line compared with the noncancerous cells, showing total absence of mitosis in the Jurkat cell line treated with fraction F8 (100μg/mL).RESUMEN: Introducción: Diversas especies de Chrysobalanaceae mostrado una variedad de actividades biológicas que constituyen una fuente de compuestos interesantes desde el punto de vista farmacológico Objetivo: Valorar el potencial bioactivo de las fracciones de L.arborea en las líneas celulares Jurkat y CHO-K1. Métodos: Todos los experimentos se realizaron en las líneas Jurkat y CHO-K1 (líneas celulares de mamíferos). Se evaluó la citotoxicidad de las fracciones mediante ensayos colorimétricos con sales de tetrazolio y azul de Tripán. La genotoxicidad se determinó con la prueba de intercambio de cromátides hermanas. Se calculó el efecto antiproliferativo por medio del ensayo clonogénico y la progresión del ciclo celular. Asimismo, la valoración de la cinética proliferativa se basó en el intercambio de cromátides hermanas en fracciones de L. arbórea. Resultados: El valor de la CI50 de las fracciones F8 y F10 fue de 100 µg/mL aproximadamente para las líneas celulares Jurkat y CHO-K1. La citotoxicidad de las fracciones evaluadas trajo como resultado la reducción de la viabilidad celular y una menor capacidad de clonación. La fracción F8 en especial mostró un efecto genotóxico reflejado en los incrementos de la frecuencia de intercambio de las cromátides hermanas, particularmente en las células Jurkat. Conclusiones: Las células Jurkat y CHO-K1 muestran con claridad la existencia de citotoxicidad y genotoxicidad dependiente de la dosis, si bien el efecto es más severo en la línea de células linfoides en comparación con las células no cancerosas, lo que demuestra la ausencia total de mitosis en la línea celular Jurkat que recibió tratamiento con la fracción F8 (100 µg/mL).COL001504312 páginasapplication/pdfengCentro Nacional de Información de Ciencias MédicasLa Habana, Cubahttp://creativecommons.org/licenses/by-nc/2.5/co/https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Licania arborea fraction bioactive potential assessment in jurkat and cho-k1 cell linesValoración del potencial bioactivo de la fracción de Licania arbórea en las líneas celulares Jurkat y Cho-K1Artículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionGenotoxicidadGenotoxicityLínea CelularCell LineCitotoxicidadCytotoxicityhttp://aims.fao.org/aos/agrovoc/c_34251https://id.nlm.nih.gov/mesh/D002460Rev. Cuba. 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