Viability of Basidiomycete fungal strains under different conservation methods : Crypreservation vs. freeze-drying processes
ABSTRACT: Four basidiomycete fungi; Agaricus blazei Murrill (Agaricomycetideae), Ganoderma lucidum (W.Curt.: Fr.) P. Karst., Grifola frondosa (Dicks.: Fr.) S.F. Gray (Higher Basidiomycetes), and Pleurotus pulmonarius (Fr.) Quél. (Agaricomycetideae) were evaluated using three conservation methods for...
- Autores:
-
Palacio Barrera, Ana María
Gutiérrez López, Yessica
Rojas Vahos, Diego Fernando
Atehortúa Garcés, Lucía
Zapata Ocampo, Paola Andrea
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2014
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/10156
- Acceso en línea:
- http://hdl.handle.net/10495/10156
- Palabra clave:
- Criopreservación
Cryopreservation
Liofilización
Freeze-drying
Basidiomycetos
Basidiomycetes
Biomasa
Biomass
Exopolisacáridos
Exopolysaccharides
Intrapolisacáridos
http://aims.fao.org/aos/agrovoc/c_67816500
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/2.5/co/
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| dc.title.spa.fl_str_mv |
Viability of Basidiomycete fungal strains under different conservation methods : Crypreservation vs. freeze-drying processes |
| dc.title.translated.spa.fl_str_mv |
Viabilidad de cepas de hongos Basidiomycetes bajo diferentes técnicas de conservación : procesos de crioconservación vs. liofilización |
| title |
Viability of Basidiomycete fungal strains under different conservation methods : Crypreservation vs. freeze-drying processes |
| spellingShingle |
Viability of Basidiomycete fungal strains under different conservation methods : Crypreservation vs. freeze-drying processes Criopreservación Cryopreservation Liofilización Freeze-drying Basidiomycetos Basidiomycetes Biomasa Biomass Exopolisacáridos Exopolysaccharides Intrapolisacáridos http://aims.fao.org/aos/agrovoc/c_67816500 |
| title_short |
Viability of Basidiomycete fungal strains under different conservation methods : Crypreservation vs. freeze-drying processes |
| title_full |
Viability of Basidiomycete fungal strains under different conservation methods : Crypreservation vs. freeze-drying processes |
| title_fullStr |
Viability of Basidiomycete fungal strains under different conservation methods : Crypreservation vs. freeze-drying processes |
| title_full_unstemmed |
Viability of Basidiomycete fungal strains under different conservation methods : Crypreservation vs. freeze-drying processes |
| title_sort |
Viability of Basidiomycete fungal strains under different conservation methods : Crypreservation vs. freeze-drying processes |
| dc.creator.fl_str_mv |
Palacio Barrera, Ana María Gutiérrez López, Yessica Rojas Vahos, Diego Fernando Atehortúa Garcés, Lucía Zapata Ocampo, Paola Andrea |
| dc.contributor.author.none.fl_str_mv |
Palacio Barrera, Ana María Gutiérrez López, Yessica Rojas Vahos, Diego Fernando Atehortúa Garcés, Lucía Zapata Ocampo, Paola Andrea |
| dc.contributor.researchgroup.spa.fl_str_mv |
Biotecnología |
| dc.subject.decs.none.fl_str_mv |
Criopreservación Cryopreservation |
| topic |
Criopreservación Cryopreservation Liofilización Freeze-drying Basidiomycetos Basidiomycetes Biomasa Biomass Exopolisacáridos Exopolysaccharides Intrapolisacáridos http://aims.fao.org/aos/agrovoc/c_67816500 |
| dc.subject.lemb.none.fl_str_mv |
Liofilización Freeze-drying Basidiomycetos Basidiomycetes Biomasa Biomass |
| dc.subject.agrovoc.none.fl_str_mv |
Exopolisacáridos Exopolysaccharides |
| dc.subject.proposal.spa.fl_str_mv |
Intrapolisacáridos |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_67816500 |
| description |
ABSTRACT: Four basidiomycete fungi; Agaricus blazei Murrill (Agaricomycetideae), Ganoderma lucidum (W.Curt.: Fr.) P. Karst., Grifola frondosa (Dicks.: Fr.) S.F. Gray (Higher Basidiomycetes), and Pleurotus pulmonarius (Fr.) Quél. (Agaricomycetideae) were evaluated using three conservation methods for 12 months, recording their viability in order to establish the best conservation method. Growth kinetics, biomass, and polysaccharide production were studied. The conservation methods implemented included: distilled wáter at 24 ºC; sawdust and rice bran with 10% glicerol at -20 ºC, sawdustand rice bran with 10% glicerol at -80 ºC; and freeze-drying of biomass with trehalose or simmed milk. After conducting the analysis of the results after 12 months of conservation, we determined that the distilled water treatment at 24 ºC was the best conservation method with the highest percentage of recoverability, at 83.3% during the 12 month, followed by the cryoconservation treatment at 80º C, where 75% were recovered with no negative effects on biomass and polysaccharide production. The -20 ºC and freeze-drying treatments were not effective; with cryoconservation at -20 ºC treatment, strain recovery only occurred during the first month and with freeze-drying it was not possible to recover any strains during the entire 12-month period evaluated. |
| publishDate |
2014 |
| dc.date.issued.none.fl_str_mv |
2014 |
| dc.date.accessioned.none.fl_str_mv |
2018-10-01T18:52:38Z |
| dc.date.available.none.fl_str_mv |
2018-10-01T18:52:38Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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Palacio A, Gutiérrez Y, Rojas D, Atehortúa L, Zapata P. Viability of Basidiomycete fungal strains under different conservation methods: Crypreservation vs. freeze-drying processes. Actual. Biol. 2014;100(36):13-21. |
| dc.identifier.issn.none.fl_str_mv |
0304-3584 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/10156 |
| dc.identifier.eissn.none.fl_str_mv |
2145-7166 |
| identifier_str_mv |
Palacio A, Gutiérrez Y, Rojas D, Atehortúa L, Zapata P. Viability of Basidiomycete fungal strains under different conservation methods: Crypreservation vs. freeze-drying processes. Actual. Biol. 2014;100(36):13-21. 0304-3584 2145-7166 |
| url |
http://hdl.handle.net/10495/10156 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Actual. Biol. |
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21 |
| dc.relation.citationissue.spa.fl_str_mv |
100 |
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13 |
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36 |
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Actualidades Biológicas |
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https://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
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info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/co/ https://creativecommons.org/licenses/by-nc-sa/4.0/ Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) http://purl.org/coar/access_right/c_abf2 |
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Universidad de Antioquia, Facultad de Ciencias Exactas y Naturales, Instituto de Biología |
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Medellín, Colombia |
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Universidad de Antioquia |
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Palacio Barrera, Ana MaríaGutiérrez López, YessicaRojas Vahos, Diego FernandoAtehortúa Garcés, LucíaZapata Ocampo, Paola AndreaBiotecnología2018-10-01T18:52:38Z2018-10-01T18:52:38Z2014Palacio A, Gutiérrez Y, Rojas D, Atehortúa L, Zapata P. Viability of Basidiomycete fungal strains under different conservation methods: Crypreservation vs. freeze-drying processes. Actual. Biol. 2014;100(36):13-21.0304-3584http://hdl.handle.net/10495/101562145-7166ABSTRACT: Four basidiomycete fungi; Agaricus blazei Murrill (Agaricomycetideae), Ganoderma lucidum (W.Curt.: Fr.) P. Karst., Grifola frondosa (Dicks.: Fr.) S.F. Gray (Higher Basidiomycetes), and Pleurotus pulmonarius (Fr.) Quél. (Agaricomycetideae) were evaluated using three conservation methods for 12 months, recording their viability in order to establish the best conservation method. Growth kinetics, biomass, and polysaccharide production were studied. The conservation methods implemented included: distilled wáter at 24 ºC; sawdust and rice bran with 10% glicerol at -20 ºC, sawdustand rice bran with 10% glicerol at -80 ºC; and freeze-drying of biomass with trehalose or simmed milk. After conducting the analysis of the results after 12 months of conservation, we determined that the distilled water treatment at 24 ºC was the best conservation method with the highest percentage of recoverability, at 83.3% during the 12 month, followed by the cryoconservation treatment at 80º C, where 75% were recovered with no negative effects on biomass and polysaccharide production. The -20 ºC and freeze-drying treatments were not effective; with cryoconservation at -20 ºC treatment, strain recovery only occurred during the first month and with freeze-drying it was not possible to recover any strains during the entire 12-month period evaluated.RESUMEN: Cuatro hongos basidiomycetes, Agaricus blazei (Agaricomycetideae), Ganoderma lucidum (W. Curt.: Fr.) P. Karsrt. y Grifola frondosa (Dicks.: Fr) S.F. Gray (Higher Basidiomycetes), y Pleurotus pulmonorius (Fr.) Quél. (Agaricomycetidae) fueron evaluados bajo tres métodos de conservación durante 12 meses, observando su viabilidad con el fin de establecer el mejor método de conservación. La cinética de crecimiento, producción de biomasa y polisacáridos fueron estudiados. Los métodos de conservación implementados incluyeron: agua destilada a 24 ºC; aserrín y salvado de arroz con glicerol 10% a -20 ºC; aserrín y salvado de arroz con glicerol 10% a -80 ºC y; liofilización de biomasa con trehalosa o leche desnatada. Luego de realizar el análisis de los resultados de 12 meses de conservación, se determinó que el tratamiento de agua destilada a 24 ºC fue el mejor método de conservación con el porcentaje de recuperabilidad más alto un 83, 3% en el mes 12, seguido por el tratamiento de criconservación a 80 º c donde se recuperó el 75%, sin afectar negativamente la producción de biomasa y polisacáridos. El tratamiento a -20º C y la liofilización no fueron efectivos; con la crionconservación a -20 ºC solo se recuperan cepas en el primer mes y con la liofilicazión no fue posible recuperar cepas en el periodo de 12 meses evaluado.COL0001084application/pdfengUniversidad de Antioquia, Facultad de Ciencias Exactas y Naturales, Instituto de BiologíaMedellín, Colombiahttps://creativecommons.org/licenses/by-nc-sa/2.5/co/https://creativecommons.org/licenses/by-nc-sa/4.0/Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Viability of Basidiomycete fungal strains under different conservation methods : Crypreservation vs. freeze-drying processesViabilidad de cepas de hongos Basidiomycetes bajo diferentes técnicas de conservación : procesos de crioconservación vs. liofilizaciónArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionCriopreservaciónCryopreservationLiofilizaciónFreeze-dryingBasidiomycetosBasidiomycetesBiomasaBiomassExopolisacáridosExopolysaccharidesIntrapolisacáridoshttp://aims.fao.org/aos/agrovoc/c_67816500Actual. 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