Galleria mellonella native and analogue peptides Gm1 and ΔGm1. II) Anti-bacterial and anti-endotoxic effects

ABSTRACT: Antimicrobial peptides (AMPs) are important components of the innate immune system of animals, plants, fungi and bacteria and are recently under discussion as promising alternatives to conventional antibiotics. We have investigated two cecropin-like synthetic peptides, Gm1, which correspon...

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Autores:: Correa Vargas, Wilmar Alexander
Manrique Moreno, Marcela María
Patiño González, Edwin Bairon
Peláez Jaramillo, Carlos Alberto
Behrends, Jochen
Marella, Chakravarthy
Gutsmann, Thomas
Brandenburg, Klaus
Heinbockel, Lena
Chakravarthy, Marella

Tipo de recurso:: Article of investigation

Fecha de publicación:: 2014

Institución:: Universidad de Antioquia

Repositorio:: Repositorio UdeA

Idioma:: eng

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Summary:	ABSTRACT: Antimicrobial peptides (AMPs) are important components of the innate immune system of animals, plants, fungi and bacteria and are recently under discussion as promising alternatives to conventional antibiotics. We have investigated two cecropin-like synthetic peptides, Gm1, which corresponds to the natural overall uncharged Galleria mellonella native peptide and ΔGm1, a modified overall positively charged Gm1 variant. We have analysed these peptides for their potential to inhibit the endotoxin-induced secretion of tumour necrosis factor-α (TNF-α) from human mononuclear cells. Furthermore, in a conventional microbiological assay, the ability of these peptides to inhibit the growth of the rough mutant bacteria Salmonella enterica Minnesota R60 and the polymyxin B-resistant Proteus mirabilis R45 was investigated and atomic force microscopy (AFM) measurements were performed to characterize the morphology of the bacteria treated by the two peptides. We have also studied their cytotoxic properties in a haemolysis assay to clarify potential toxic effects. Our data revealed for both peptides minor anti-inflammatory (anti-endotoxin) activity, but demonstrated anti- microbial activity with differences depending on the endotoxin composition of the respective bacteria. In accor- dance with the antimicrobial assay, AFM data revealed a stronger morphology change of the R45 bacteria than for the R60. Furthermore, Gm1 had a stronger effect on the bacteria than ΔGm1, leading to a different morphology regarding indentations and coalescing of bacterial structures. The findings verify the biophysical measurements with the peptides on model systems. Both peptides lack any haemolytic activity up to an amount of 100 μg/ml, making them suitable as new anti-infective agents.

Galleria mellonella native and analogue peptides Gm1 and ΔGm1. II) Anti-bacterial and anti-endotoxic effects

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