Inmovilización covalente en quitosano de enzimas ligninolíticas obtenidas a partir de hongos de la podredumbre blanca y su aplicación en la degradación de contaminantes emergentes como acetaminofén e ibuprofeno
El agua de buena calidad es esencial para mantener el bienestar humano y el equilibrio ecológico de los ecosistemas con miras al desarrollo sostenible. Una subclase de productos químicos orgánicos que se detectan cada vez con más frecuencia en cuerpos de agua y que son de importancia porque alteran...
- Autores:
-
Flórez Restrepo, María Alejandra
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- spa
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/47590
- Acceso en línea:
- https://hdl.handle.net/10495/47590
- Palabra clave:
- Impacto ambiental
Environmental Impact
Podredumbre blanca (madera)
white rot (wood)
Biodegradación
biodegradation
Hongos de la podredumbre blanca
Enzimas ligninolíticas
Contaminantes emergentes
Inmovilización en quitosano
Ligninolytic enzymes
Emerging contaminants
Chitosan immobilization
http://aims.fao.org/aos/agrovoc/c_25100
http://aims.fao.org/aos/agrovoc/c_9261
ODS 3: Salud y bienestar. Garantizar una vida sana y promover el bienestar de todos a todas las edades
ODS 6: Agua limpia y saneamiento. Garantizar la disponibilidad y la gestión sostenible del agua y el saneamiento para todos
ODS 9: Industria, innovación e infraestructura. Construir infraestructuras resilientes, promover la industrialización inclusiva y sostenible y fomentar la innovación
ODS 11: Ciudades y comunidades sostenibles. Lograr que las ciudades y los asentamientos humanos sean inclusivos, seguros, resilientes y sostenibles
ODS 14: Vida submarina. Conservar y utilizar sosteniblemente los océanos, los mares y los recursos marinos para el desarrollo sostenible
ODS 15: Vida de ecosistemas terrestres. Proteger, restablecer y promover el uso sostenible de los ecosistemas terrestres, gestionar sosteniblemente los bosques, luchar contra la desertificación, detener e invertir la degradación de las tierras y detener la pérdida de biodiversidad
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/4.0/
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| dc.title.spa.fl_str_mv |
Inmovilización covalente en quitosano de enzimas ligninolíticas obtenidas a partir de hongos de la podredumbre blanca y su aplicación en la degradación de contaminantes emergentes como acetaminofén e ibuprofeno |
| dc.title.translated.none.fl_str_mv |
Covalent immobilization in chitosan of ligninolytic enzymes obtained from white-rot fungi and their application in the degradation of emerging contaminants such as acetaminophen and ibuprofen |
| title |
Inmovilización covalente en quitosano de enzimas ligninolíticas obtenidas a partir de hongos de la podredumbre blanca y su aplicación en la degradación de contaminantes emergentes como acetaminofén e ibuprofeno |
| spellingShingle |
Inmovilización covalente en quitosano de enzimas ligninolíticas obtenidas a partir de hongos de la podredumbre blanca y su aplicación en la degradación de contaminantes emergentes como acetaminofén e ibuprofeno Impacto ambiental Environmental Impact Podredumbre blanca (madera) white rot (wood) Biodegradación biodegradation Hongos de la podredumbre blanca Enzimas ligninolíticas Contaminantes emergentes Inmovilización en quitosano Ligninolytic enzymes Emerging contaminants Chitosan immobilization http://aims.fao.org/aos/agrovoc/c_25100 http://aims.fao.org/aos/agrovoc/c_9261 ODS 3: Salud y bienestar. Garantizar una vida sana y promover el bienestar de todos a todas las edades ODS 6: Agua limpia y saneamiento. Garantizar la disponibilidad y la gestión sostenible del agua y el saneamiento para todos ODS 9: Industria, innovación e infraestructura. Construir infraestructuras resilientes, promover la industrialización inclusiva y sostenible y fomentar la innovación ODS 11: Ciudades y comunidades sostenibles. Lograr que las ciudades y los asentamientos humanos sean inclusivos, seguros, resilientes y sostenibles ODS 14: Vida submarina. Conservar y utilizar sosteniblemente los océanos, los mares y los recursos marinos para el desarrollo sostenible ODS 15: Vida de ecosistemas terrestres. Proteger, restablecer y promover el uso sostenible de los ecosistemas terrestres, gestionar sosteniblemente los bosques, luchar contra la desertificación, detener e invertir la degradación de las tierras y detener la pérdida de biodiversidad |
| title_short |
Inmovilización covalente en quitosano de enzimas ligninolíticas obtenidas a partir de hongos de la podredumbre blanca y su aplicación en la degradación de contaminantes emergentes como acetaminofén e ibuprofeno |
| title_full |
Inmovilización covalente en quitosano de enzimas ligninolíticas obtenidas a partir de hongos de la podredumbre blanca y su aplicación en la degradación de contaminantes emergentes como acetaminofén e ibuprofeno |
| title_fullStr |
Inmovilización covalente en quitosano de enzimas ligninolíticas obtenidas a partir de hongos de la podredumbre blanca y su aplicación en la degradación de contaminantes emergentes como acetaminofén e ibuprofeno |
| title_full_unstemmed |
Inmovilización covalente en quitosano de enzimas ligninolíticas obtenidas a partir de hongos de la podredumbre blanca y su aplicación en la degradación de contaminantes emergentes como acetaminofén e ibuprofeno |
| title_sort |
Inmovilización covalente en quitosano de enzimas ligninolíticas obtenidas a partir de hongos de la podredumbre blanca y su aplicación en la degradación de contaminantes emergentes como acetaminofén e ibuprofeno |
| dc.creator.fl_str_mv |
Flórez Restrepo, María Alejandra |
| dc.contributor.advisor.none.fl_str_mv |
Segura Sánchez, Freimar López Legarda, Xiomara |
| dc.contributor.author.none.fl_str_mv |
Flórez Restrepo, María Alejandra |
| dc.contributor.researchgroup.none.fl_str_mv |
BIOPOLIMER |
| dc.contributor.jury.none.fl_str_mv |
Cinto, Isabel Esther Hernández Luna, Carlos Eduardo |
| dc.subject.lemb.none.fl_str_mv |
Impacto ambiental Environmental Impact |
| topic |
Impacto ambiental Environmental Impact Podredumbre blanca (madera) white rot (wood) Biodegradación biodegradation Hongos de la podredumbre blanca Enzimas ligninolíticas Contaminantes emergentes Inmovilización en quitosano Ligninolytic enzymes Emerging contaminants Chitosan immobilization http://aims.fao.org/aos/agrovoc/c_25100 http://aims.fao.org/aos/agrovoc/c_9261 ODS 3: Salud y bienestar. Garantizar una vida sana y promover el bienestar de todos a todas las edades ODS 6: Agua limpia y saneamiento. Garantizar la disponibilidad y la gestión sostenible del agua y el saneamiento para todos ODS 9: Industria, innovación e infraestructura. Construir infraestructuras resilientes, promover la industrialización inclusiva y sostenible y fomentar la innovación ODS 11: Ciudades y comunidades sostenibles. Lograr que las ciudades y los asentamientos humanos sean inclusivos, seguros, resilientes y sostenibles ODS 14: Vida submarina. Conservar y utilizar sosteniblemente los océanos, los mares y los recursos marinos para el desarrollo sostenible ODS 15: Vida de ecosistemas terrestres. Proteger, restablecer y promover el uso sostenible de los ecosistemas terrestres, gestionar sosteniblemente los bosques, luchar contra la desertificación, detener e invertir la degradación de las tierras y detener la pérdida de biodiversidad |
| dc.subject.agrovoc.none.fl_str_mv |
Podredumbre blanca (madera) white rot (wood) Biodegradación biodegradation |
| dc.subject.proposal.spa.fl_str_mv |
Hongos de la podredumbre blanca Enzimas ligninolíticas Contaminantes emergentes Inmovilización en quitosano |
| dc.subject.proposal.eng.fl_str_mv |
Ligninolytic enzymes Emerging contaminants Chitosan immobilization |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_25100 http://aims.fao.org/aos/agrovoc/c_9261 |
| dc.subject.ods.none.fl_str_mv |
ODS 3: Salud y bienestar. Garantizar una vida sana y promover el bienestar de todos a todas las edades ODS 6: Agua limpia y saneamiento. Garantizar la disponibilidad y la gestión sostenible del agua y el saneamiento para todos ODS 9: Industria, innovación e infraestructura. Construir infraestructuras resilientes, promover la industrialización inclusiva y sostenible y fomentar la innovación ODS 11: Ciudades y comunidades sostenibles. Lograr que las ciudades y los asentamientos humanos sean inclusivos, seguros, resilientes y sostenibles ODS 14: Vida submarina. Conservar y utilizar sosteniblemente los océanos, los mares y los recursos marinos para el desarrollo sostenible ODS 15: Vida de ecosistemas terrestres. Proteger, restablecer y promover el uso sostenible de los ecosistemas terrestres, gestionar sosteniblemente los bosques, luchar contra la desertificación, detener e invertir la degradación de las tierras y detener la pérdida de biodiversidad |
| description |
El agua de buena calidad es esencial para mantener el bienestar humano y el equilibrio ecológico de los ecosistemas con miras al desarrollo sostenible. Una subclase de productos químicos orgánicos que se detectan cada vez con más frecuencia en cuerpos de agua y que son de importancia porque alteran negativamente los ecosistemas se han clasificado como contaminantes emergentes. Entre ellos, se encuentran bajo especial atención algunos compuestos farmacéuticos porque poseen actividad biológica y algunos de alto consumo como el acetaminofén e ibuprofeno, se están liberando en grandes cantidades al ambiente. Debido a esto, se están desarrollando y estudiando diversos métodos y técnicas para degradar contaminantes, entre los cuales, se han evaluado diferentes tipos de enzimas con capacidad oxidativa. Entre los diversos organismos estudiados en biorremediación, los hongos han llamado particular atención, y entre ellos, los que producen pudrición blanca de la madera gracias a sus enzimas ligninolíticas que han sido las más promisorias y estudiadas. Si bien, las enzimas libres tienen aplicabilidad, la investigación de enzimas inmovilizadas es de gran interés industrial, debido al hecho de que éstas pueden ser utilizadas por más de un ciclo catalítico, es decir, reutilizarse manteniendo una eficiencia satisfactoria o incluso utilizarse en procesos en continuo. Inicialmente, se estudió la producción de enzimas ligninolíticas de 17 hongos de la podredumbre blanca previamente aislados en un bosque húmedo tropical colombiano, incubando los cultivos durante 6 semanas en un medio liquido rico en material ligninocelulósico compuesto de residuos agroindustriales. Se encontraron principalmente géneros de Ganoderma spp. y Lentinus sp., con la capacidad de producir lacasa y manganeso peroxidasa. En la siguiente etapa, se realizó producción enzimática cultivando el aislado Ganoderma parvulum en biorreactor de 5L. Posteriormente, las enzimas se purificaron parcialmente y se inmovilizaron de manera covalente en microesferas de quitosano. Para tal fin, las microesferas obtenidas fueron activadas utilizando un agente entrecruzante y puestas en contacto con el extracto enzimático. Se encontró que entre los parámetros evaluados se obtuvo mayor eficiencia de inmovilización con 10% de glutaraldehído, 60 minutos de tiempo de reticulación y 10.000 U/L de lacasas. Además, se evaluó la estabilidad operativa de las macropartículas con enzimas y la aplicación de éstas en la biotransformación de acetaminofén e ibuprofeno. Encontrando hasta un 98% de biosorción/biotransformación del acetaminofén asociado a las enzimas inmovilizadas después de cuatro horas de reacción. Concluyendo así en la posible utilidad del sistema evaluado en tratamientos de biorremediación, debido a que este estudio proporcionó un enfoque de tratamiento para degradar contaminantes emergentes como fármacos de alto consumo como acetaminofén e ibuprofeno, con una alta eficacia, resultados que son buscados debido a la presencia ubicua de estos fármacos en la naturaleza y sus propiedades toxicológicas en el ambiente. No se descarta la capacidad del sistema para degradar muchos otros tipos de contaminantes presentes en cuerpos de agua ya que existen reportes de la capacidad de las enzimas ligninolíticas para degradar otros tipos de compuestos como colorantes, pesticidas, disruptores endocrinos, entre otros. |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2025-10-08T12:52:12Z |
| dc.type.none.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.redcol.none.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| dc.type.content.none.fl_str_mv |
Text |
| dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.none.fl_str_mv |
info:eu-repo/semantics/draft |
| status_str |
draft |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/47590 |
| url |
https://hdl.handle.net/10495/47590 |
| dc.language.iso.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.none.fl_str_mv |
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Segura Sánchez, FreimarLópez Legarda, XiomaraFlórez Restrepo, María AlejandraBIOPOLIMERCinto, Isabel EstherHernández Luna, Carlos Eduardo2025-10-08T12:52:12Z2023https://hdl.handle.net/10495/47590El agua de buena calidad es esencial para mantener el bienestar humano y el equilibrio ecológico de los ecosistemas con miras al desarrollo sostenible. Una subclase de productos químicos orgánicos que se detectan cada vez con más frecuencia en cuerpos de agua y que son de importancia porque alteran negativamente los ecosistemas se han clasificado como contaminantes emergentes. Entre ellos, se encuentran bajo especial atención algunos compuestos farmacéuticos porque poseen actividad biológica y algunos de alto consumo como el acetaminofén e ibuprofeno, se están liberando en grandes cantidades al ambiente. Debido a esto, se están desarrollando y estudiando diversos métodos y técnicas para degradar contaminantes, entre los cuales, se han evaluado diferentes tipos de enzimas con capacidad oxidativa. Entre los diversos organismos estudiados en biorremediación, los hongos han llamado particular atención, y entre ellos, los que producen pudrición blanca de la madera gracias a sus enzimas ligninolíticas que han sido las más promisorias y estudiadas. Si bien, las enzimas libres tienen aplicabilidad, la investigación de enzimas inmovilizadas es de gran interés industrial, debido al hecho de que éstas pueden ser utilizadas por más de un ciclo catalítico, es decir, reutilizarse manteniendo una eficiencia satisfactoria o incluso utilizarse en procesos en continuo. Inicialmente, se estudió la producción de enzimas ligninolíticas de 17 hongos de la podredumbre blanca previamente aislados en un bosque húmedo tropical colombiano, incubando los cultivos durante 6 semanas en un medio liquido rico en material ligninocelulósico compuesto de residuos agroindustriales. Se encontraron principalmente géneros de Ganoderma spp. y Lentinus sp., con la capacidad de producir lacasa y manganeso peroxidasa. En la siguiente etapa, se realizó producción enzimática cultivando el aislado Ganoderma parvulum en biorreactor de 5L. Posteriormente, las enzimas se purificaron parcialmente y se inmovilizaron de manera covalente en microesferas de quitosano. Para tal fin, las microesferas obtenidas fueron activadas utilizando un agente entrecruzante y puestas en contacto con el extracto enzimático. Se encontró que entre los parámetros evaluados se obtuvo mayor eficiencia de inmovilización con 10% de glutaraldehído, 60 minutos de tiempo de reticulación y 10.000 U/L de lacasas. Además, se evaluó la estabilidad operativa de las macropartículas con enzimas y la aplicación de éstas en la biotransformación de acetaminofén e ibuprofeno. Encontrando hasta un 98% de biosorción/biotransformación del acetaminofén asociado a las enzimas inmovilizadas después de cuatro horas de reacción. Concluyendo así en la posible utilidad del sistema evaluado en tratamientos de biorremediación, debido a que este estudio proporcionó un enfoque de tratamiento para degradar contaminantes emergentes como fármacos de alto consumo como acetaminofén e ibuprofeno, con una alta eficacia, resultados que son buscados debido a la presencia ubicua de estos fármacos en la naturaleza y sus propiedades toxicológicas en el ambiente. No se descarta la capacidad del sistema para degradar muchos otros tipos de contaminantes presentes en cuerpos de agua ya que existen reportes de la capacidad de las enzimas ligninolíticas para degradar otros tipos de compuestos como colorantes, pesticidas, disruptores endocrinos, entre otros.Good water quality is essential to maintain human well-being and the ecological balance of ecosystems with a view to sustainable development. A subclass of organic chemicals that are being detected with increasing frequency in water bodies and that are of importance because they negatively alter ecosystems, have been classified as emerging pollutants; Among them, some pharmaceutical products are under special attention because they have biological activity and some of high consumption, as acetaminophen and ibuprofen, are being released in large quantities into the environment. Due to this, various methods and techniques have been developed and studied to degrade contaminants, among which, different types of enzymes with oxidative capacity have been evaluated. Fungi have drawn particular attention, and among them, those that produce white-rot thanks to their ligninolytic enzymes, the most promising and studied. Although, as free enzymes have applicability, research on immobilized enzymes is of great industrial interest, due to the fact that they can be used for more than one catalytic cycle, that is, reused while maintaining satisfactory efficiency or even used in continuous processes. Initially, the production of ligninolytic enzymes was studied, by 17 white-rot fungi previously isolated in a Colombian tropical humid forest, incubating the cultures for 6 weeks in liquid medium rich in ligninocellulosic material composed of agro-industrial waste. Mainly genera of Ganoderma spp., and Lentinus sp. were found, with the ability to produce laccase and manganese peroxidase. In the next stage, enzyme production was carried out cultivating the Ganoderma parvulum isolate in a 5L bioreactor. Subsequently, the enzymes were partially purified and covalently immobilized on chitosan microspheres. For this purpose, the microspheres obtained were activated using a crosslinking agent and placed in contact with the enzyme extract. It was found that among the parameters evaluated, the best immobilization efficiency was obtained with 10% glutaraldehyde, 60 minutes of crosslinking time and 10,000 U/L of laccase. In addition, the operational stability of the microparticles with enzymes and their application in the biotransformation of acetaminophen and ibuprofen were evaluated. Finding up to 98% biosorption/biotransformation of acetaminophen associated with immobilized enzymes after four hours of reaction. Thus, concluding in the possible usefulness of the evaluated system in bioremediation treatments, because this study has an approach to degrade emerging contaminants such as high-consumption drugs like acetaminophen with high efficacy, results that are sought due to the ubiquitous presence of this drugs in nature and their toxicological properties in the environment. The ability of the system to degrade many other types of pollutants present in bodies of water is not ruled out, since there are reports of the ability of ligninolytic enzymes to degrade other types of compounds as dyes, pesticides, endocrine disruptors, among others.Evaluación del potencial ligninolítico de varias especies de hongos basidiomicetos y bioprocesos para aplicaciones biotecnológicasEvaluación del potencial de hongos de Colombia para el desarrollo de bioprocesos y la obtención de metabolitos para aplicaciones biotecnológicas, farmacéuticas, cosméticas y alimentariasBiotecnologíaCOL0065152El presente trabajo de investigación, realizado en el marco del programa de Maestría en Ciencias Farmacéuticas y Alimentarias, línea Biotecnología, ha dado lugar a los siguientes productos de divulgación y reconocimientos: Publicaciones científicas derivadas: • Flórez-Restrepo, M. A., López-Legarda, X., Rostro-Alanis, M. de J., Parra-Saldívar, R., & Segura-Sánchez, F. (2025). Biotransformation of Acetaminophen by Ganoderma parvulum Ligninolytic Enzymes Immobilized on Chitosan Microspheres. Fermentation, 11(7), 387. https://doi.org/10.3390/FERMENTATION11070387 • Flórez-Restrepo, M. A., López-Legarda, X., & Segura-Sánchez, F. (2025). Bioremediation of emerging pharmaceutical pollutants acetaminophen and ibuprofen by white-rot fungi – A review. Science of The Total Environment, 977, 179379. https://doi.org/10.1016/J.SCITOTENV.2025.179379 Eventos de divulgación científica: • Parte de los resultados de esta investigación fueron presentados en el XI Congreso Latinoamericano de Micología, celebrado en la ciudad de Panamá (República de Panamá), del 7 al 10 de agosto de 2023, organizado por la Asociación Latinoamericana de Micología. Reconocimiento académico: • Este trabajo fue evaluado por un jurado internacional conformado por académicos de Argentina y México, y recibió la máxima calificación. Los jurados recomendaron otorgar la distinción Summa Cum Laude, la cual fue aprobada por el programa de posgrado. • Obtuvo un reconocimiento en Accésit (segundo lugar) en la modalidad de Mejor Trabajo Fin de Máster en el área Científico-Técnica en los XVI Premios Iberoamericanos La Rábida 2024, convocados por el Grupo de Universidades Iberoamericanas La Rábida, en colaboración con la Universidad Internacional de Andalucía (España) y la Fundación Cajasol, entre 103 postulaciones evaluadas.MaestríaMagíster en Ciencias Farmacéuticas y Alimentarias135 páginasapplication/pdfspaUniversidad de AntioquiaMaestría en Ciencias Farmacéuticas y AlimentariasDepartamento de Extensión y PosgradosMedellín, ColombiaFacultad de Ciencias Farmacéuticas y AlimentariasCampus Medellín - Ciudad Universitariahttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessAttribution-NonCommercial-ShareAlike 4.0 Internationalhttp://purl.org/coar/access_right/c_abf2Inmovilización covalente en quitosano de enzimas ligninolíticas obtenidas a partir de hongos de la podredumbre blanca y su aplicación en la degradación de contaminantes emergentes como acetaminofén e ibuprofenoCovalent immobilization in chitosan of ligninolytic enzymes obtained from white-rot fungi and their application in the degradation of emerging contaminants such as acetaminophen and ibuprofenTrabajo de grado - Maestríahttp://purl.org/redcol/resource_type/TMTexthttp://purl.org/coar/version/c_b1a7d7d4d402bcceinfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/draftAfreen, S., Anwer, R., Singh, R. 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In Environmental Science and Pollution Research (Vol. 25, Issue 22, pp. 21498–21524). Springer Verlag. https://doi.org/10.1007/s11356-018-2517-xImpacto ambientalEnvironmental ImpactPodredumbre blanca (madera)white rot (wood)BiodegradaciónbiodegradationHongos de la podredumbre blancaEnzimas ligninolíticasContaminantes emergentesInmovilización en quitosanoLigninolytic enzymesEmerging contaminantsChitosan immobilizationhttp://aims.fao.org/aos/agrovoc/c_25100http://aims.fao.org/aos/agrovoc/c_9261ODS 3: Salud y bienestar. Garantizar una vida sana y promover el bienestar de todos a todas las edadesODS 6: Agua limpia y saneamiento. Garantizar la disponibilidad y la gestión sostenible del agua y el saneamiento para todosODS 9: Industria, innovación e infraestructura. Construir infraestructuras resilientes, promover la industrialización inclusiva y sostenible y fomentar la innovaciónODS 11: Ciudades y comunidades sostenibles. 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Vwcm9kdWNpciBsYSBPYnJhIGluY29ycG9yYWRhIGVuIGxhcyBPYnJhcyBDb2xlY3RpdmFzLgpiLiBEaXN0cmlidWlyIGNvcGlhcyBvIGZvbm9ncmFtYXMgZGUgbGFzIE9icmFzLCBleGhpYmlybGFzIHDDumJsaWNhbWVudGUsIGVqZWN1dGFybGFzIHDDumJsaWNhbWVudGUgeS9vIHBvbmVybGFzIGEgZGlzcG9zaWNpw7NuIHDDumJsaWNhLCBpbmNsdXnDqW5kb2xhcyBjb21vIGluY29ycG9yYWRhcyBlbiBPYnJhcyBDb2xlY3RpdmFzLCBzZWfDum4gY29ycmVzcG9uZGEuCmMuIERpc3RyaWJ1aXIgY29waWFzIGRlIGxhcyBPYnJhcyBEZXJpdmFkYXMgcXVlIHNlIGdlbmVyZW4sIGV4aGliaXJsYXMgcMO6YmxpY2FtZW50ZSwgZWplY3V0YXJsYXMgcMO6YmxpY2FtZW50ZSB5L28gcG9uZXJsYXMgYSBkaXNwb3NpY2nDs24gcMO6YmxpY2EuCgpMb3MgZGVyZWNob3MgbWVuY2lvbmFkb3MgYW50ZXJpb3JtZW50ZSBwdWVkZW4gc2VyIGVqZXJjaWRvcyBlbiB0b2RvcyBsb3MgbWVkaW9zIHkgZm9ybWF0b3MsIGFjdHVhbG1lbnRlIGNvbm9jaWRvcyBvIHF1ZSBzZSBpbnZlbnRlbiBlbiBlbCBmdXR1cm8uIExvcyBkZXJlY2hvcyBhbnRlcyBtZW5jaW9uYWRvcyBpbmNsdXllbiBlbCBkZXJlY2hvIGEgcmVhbGl6YXIgZGljaGFzIG1vZGlmaWNhY2lvbmVzIGVuIGxhIG1lZGlkYSBxdWUgc2VhbiB0w6ljbmljYW1lbnRlIG5lY2VzYXJpYXMgcGFyYSBlamVyY2VyIGxvcyBkZXJlY2hvcyBlbiBvdHJvIG1lZGlvIG8gZm9ybWF0b3MsIHBlcm8gZGUgb3RyYSBtYW5lcmEgdXN0ZWQgbm8gZXN0w6EgYXV0b3JpemFkbyBwYXJhIHJlYWxpemFyIG9icmFzIGRlcml2YWRhcy4gVG9kb3MgbG9zIGRlcmVjaG9zIG5vIG90b3JnYWRvcyBleHByZXNhbWVudGUgcG9yIGVsIExpY2VuY2lhbnRlIHF1ZWRhbiBwb3IgZXN0ZSBtZWRpbyByZXNlcnZhZG9zLCBpbmNsdXllbmRvIHBlcm8gc2luIGxpbWl0YXJzZSBhIGFxdWVsbG9zIHF1ZSBzZSBtZW5jaW9uYW4gZW4gbGFzIHNlY2Npb25lcyA0KGQpIHkgNChlKS4KICAgIAo0LiBSZXN0cmljY2lvbmVzLgpMYSBsaWNlbmNpYSBvdG9yZ2FkYSBlbiBsYSBhbnRlcmlvciBTZWNjacOzbiAzIGVzdMOhIGV4cHJlc2FtZW50ZSBzdWpldGEgeSBsaW1pdGFkYSBwb3IgbGFzIHNpZ3VpZW50ZXMgcmVzdHJpY2Npb25lczoKYS4gVXN0ZWQgcHVlZGUgZGlzdHJpYnVpciwgZXhoaWJpciBww7pibGljYW1lbnRlLCBlamVjdXRhciBww7pibGljYW1lbnRlLCBvIHBvbmVyIGEgZGlzcG9zaWNpw7NuIHDDumJsaWNhIGxhIE9icmEgc8OzbG8gYmFqbyBsYXMgY29uZGljaW9uZXMgZGUgZXN0YSBMaWNlbmNpYSwgeSBVc3RlZCBkZWJlIGluY2x1aXIgdW5hIGNvcGlhIGRlIGVzdGEgbGljZW5jaWEgbyBkZWwgSWRlbnRpZmljYWRvciBVbml2ZXJzYWwgZGUgUmVjdXJzb3MgZGUgbGEgbWlzbWEgY29uIGNhZGEgY29waWEgZGUgbGEgT2JyYSBxdWUgZGlzdHJpYnV5YSwgZXhoaWJhIHDDumJsaWNhbWVudGUsIGVqZWN1dGUgcMO6YmxpY2FtZW50ZSBvIHBvbmdhIGEgZGlzcG9zaWNpw7NuIHDDumJsaWNhLiBObyBlcyBwb3NpYmxlIG9mcmVjZXIgbyBpbXBvbmVyIG5pbmd1bmEgY29uZGljacOzbiBzb2JyZSBsYSBPYnJhIHF1ZSBhbHRlcmUgbyBsaW1pdGUgbGFzIGNvbmRpY2lvbmVzIGRlIGVzdGEgTGljZW5jaWEgbyBlbCBlamVyY2ljaW8gZGUgbG9zIGRlcmVjaG9zIGRlIGxvcyBkZXN0aW5hdGFyaW9zIG90b3JnYWRvcyBlbiBlc3RlIGRvY3VtZW50by4gTm8gZXMgcG9zaWJsZSBzdWJsaWNlbmNpYXIgbGEgT2JyYS4gVXN0ZWQgZGViZSBtYW50ZW5lciBpbnRhY3RvcyB0b2RvcyBsb3MgYXZpc29zIHF1ZSBoYWdhbiByZWZlcmVuY2lhIGEgZXN0YSBMaWNlbmNpYSB5IGEgbGEgY2zDoXVzdWxhIGRlIGxpbWl0YWNpw7NuIGRlIGdhcmFudMOtYXMuIFVzdGVkIG5vIHB1ZWRlIGRpc3RyaWJ1aXIsIGV4aGliaXIgcMO6YmxpY2FtZW50ZSwgZWplY3V0YXIgcMO6YmxpY2FtZW50ZSwgbyBwb25lciBhIGRpc3Bvc2ljacOzbiBww7pibGljYSBsYSBPYnJhIGNvbiBhbGd1bmEgbWVkaWRhIHRlY25vbMOzZ2ljYSBxdWUgY29udHJvbGUgZWwgYWNjZXNvIG8gbGEgdXRpbGl6YWNpw7NuIGRlIGVsbGEgZGUgdW5hIGZvcm1hIHF1ZSBzZWEgaW5jb25zaXN0ZW50ZSBjb24gbGFzIGNvbmRpY2lvbmVzIGRlIGVzdGEgTGljZW5jaWEuIExvIGFudGVyaW9yIHNlIGFwbGljYSBhIGxhIE9icmEgaW5jb3Jwb3JhZGEgYSB1bmEgT2JyYSBDb2xlY3RpdmEsIHBlcm8gZXN0byBubyBleGlnZSBxdWUgbGEgT2JyYSBDb2xlY3RpdmEgYXBhcnRlIGRlIGxhIG9icmEgbWlzbWEgcXVlZGUgc3VqZXRhIGEgbGFzIGNvbmRpY2lvbmVzIGRlIGVzdGEgTGljZW5jaWEuIFNpIFVzdGVkIGNyZWEgdW5hIE9icmEgQ29sZWN0aXZhLCBwcmV2aW8gYXZpc28gZGUgY3VhbHF1aWVyIExpY2VuY2lhbnRlIGRlYmUsIGVuIGxhIG1lZGlkYSBkZSBsbyBwb3NpYmxlLCBlbGltaW5hciBkZSBsYSBPYnJhIENvbGVjdGl2YSBjdWFscXVpZXIgcmVmZXJlbmNpYSBhIGRpY2hvIExpY2VuY2lhbnRlIG8gYWwgQXV0b3IgT3JpZ2luYWwsIHNlZ8O6biBsbyBzb2xpY2l0YWRvIHBvciBlbCBMaWNlbmNpYW50ZSB5IGNvbmZvcm1lIGxvIGV4aWdlIGxhIGNsw6F1c3VsYSA0KGMpLgpiLiBVc3RlZCBubyBwdWVkZSBlamVyY2VyIG5pbmd1bm8gZGUgbG9zIGRlcmVjaG9zIHF1ZSBsZSBoYW4gc2lkbyBvdG9yZ2Fkb3MgZW4gbGEgU2VjY2nDs24gMyBwcmVjZWRlbnRlIGRlIG1vZG8gcXVlIGVzdMOpbiBwcmluY2lwYWxtZW50ZSBkZXN0aW5hZG9zIG8gZGlyZWN0YW1lbnRlIGRpcmlnaWRvcyBhIGNvbnNlZ3VpciB1biBwcm92ZWNobyBjb21lcmNpYWwgbyB1bmEgY29tcGVuc2FjacOzbiBtb25ldGFyaWEgcHJpdmFkYS4gRWwgaW50ZXJjYW1iaW8gZGUgbGEgT2JyYSBwb3Igb3RyYXMgb2JyYXMgcHJvdGVnaWRhcyBwb3IgZGVyZWNob3MgZGUgYXV0b3IsIHlhIHNlYSBhIHRyYXbDqXMgZGUgdW4gc2lzdGVtYSBwYXJhIGNvbXBhcnRpciBhcmNoaXZvcyBkaWdpdGFsZXMgKGRpZ2l0YWwgZmlsZS1zaGFyaW5nKSBvIGRlIGN1YWxxdWllciBvdHJhIG1hbmVyYSBubyBzZXLDoSBjb25zaWRlcmFkbyBjb21vIGVzdGFyIGRlc3RpbmFkbyBwcmluY2lwYWxtZW50ZSBvIGRpcmlnaWRvIGRpcmVjdGFtZW50ZSBhIGNvbnNlZ3VpciB1biBwcm92ZWNobyBjb21lcmNpYWwgbyB1bmEgY29tcGVuc2FjacOzbiBtb25ldGFyaWEgcHJpdmFkYSwgc2llbXByZSBxdWUgbm8gc2UgcmVhbGljZSB1biBwYWdvIG1lZGlhbnRlIHVuYSBjb21wZW5zYWNpw7NuIG1vbmV0YXJpYSBlbiByZWxhY2nDs24gY29uIGVsIGludGVyY2FtYmlvIGRlIG9icmFzIHByb3RlZ2lkYXMgcG9yIGVsIGRlcmVjaG8gZGUgYXV0b3IuCmMuIFNpIHVzdGVkIGRpc3RyaWJ1eWUsIGV4aGliZSBww7pibGljYW1lbnRlLCBlamVjdXRhIHDDumJsaWNhbWVudGUgbyBlamVjdXRhIHDDumJsaWNhbWVudGUgZW4gZm9ybWEgZGlnaXRhbCBsYSBPYnJhIG8gY3VhbHF1aWVyIE9icmEgRGVyaXZhZGEgdSBPYnJhIENvbGVjdGl2YSwgVXN0ZWQgZGViZSBtYW50ZW5lciBpbnRhY3RhIHRvZGEgbGEgaW5mb3JtYWNpw7NuIGRlIGRlcmVjaG8gZGUgYXV0b3IgZGUgbGEgT2JyYSB5IHByb3BvcmNpb25hciwgZGUgZm9ybWEgcmF6b25hYmxlIHNlZ8O6biBlbCBtZWRpbyBvIG1hbmVyYSBxdWUgVXN0ZWQgZXN0w6kgdXRpbGl6YW5kbzogKGkpIGVsIG5vbWJyZSBkZWwgQXV0b3IgT3JpZ2luYWwgc2kgZXN0w6EgcHJvdmlzdG8gKG8gc2V1ZMOzbmltbywgc2kgZnVlcmUgYXBsaWNhYmxlKSwgeS9vIChpaSkgZWwgbm9tYnJlIGRlIGxhIHBhcnRlIG8gbGFzIHBhcnRlcyBxdWUgZWwgQXV0b3IgT3JpZ2luYWwgeS9vIGVsIExpY2VuY2lhbnRlIGh1YmllcmVuIGRlc2lnbmFkbyBwYXJhIGxhIGF0cmlidWNpw7NuICh2LmcuLCB1biBpbnN0aXR1dG8gcGF0cm9jaW5hZG9yLCBlZGl0b3JpYWwsIHB1YmxpY2FjacOzbikgZW4gbGEgaW5mb3JtYWNpw7NuIGRlIGxvcyBkZXJlY2hvcyBkZSBhdXRvciBkZWwgTGljZW5jaWFudGUsIHTDqXJtaW5vcyBkZSBzZXJ2aWNpb3MgbyBkZSBvdHJhcyBmb3JtYXMgcmF6b25hYmxlczsgZWwgdMOtdHVsbyBkZSBsYSBPYnJhIHNpIGVzdMOhIHByb3Zpc3RvOyBlbiBsYSBtZWRpZGEgZGUgbG8gcmF6b25hYmxlbWVudGUgZmFjdGlibGUgeSwgc2kgZXN0w6EgcHJvdmlzdG8sIGVsIElkZW50aWZpY2Fkb3IgVW5pZm9ybWUgZGUgUmVjdXJzb3MgKFVuaWZvcm0gUmVzb3VyY2UgSWRlbnRpZmllcikgcXVlIGVsIExpY2VuY2lhbnRlIGVzcGVjaWZpY2EgcGFyYSBzZXIgYXNvY2lhZG8gY29uIGxhIE9icmEsIHNhbHZvIHF1ZSB0YWwgVVJJIG5vIHNlIHJlZmllcmEgYSBsYSBub3RhIHNvYnJlIGxvcyBkZXJlY2hvcyBkZSBhdXRvciBvIGEgbGEgaW5mb3JtYWNpw7NuIHNvYnJlIGVsIGxpY2VuY2lhbWllbnRvIGRlIGxhIE9icmE7IHkgZW4gZWwgY2FzbyBkZSB1bmEgT2JyYSBEZXJpdmFkYSwgYXRyaWJ1aXIgZWwgY3LDqWRpdG8gaWRlbnRpZmljYW5kbyBlbCB1c28gZGUgbGEgT2JyYSBlbiBsYSBPYnJhIERlcml2YWRhICh2LmcuLCAiVHJhZHVjY2nDs24gRnJhbmNlc2EgZGUgbGEgT2JyYSBkZWwgQXV0b3IgT3JpZ2luYWwsIiBvICJHdWnDs24gQ2luZW1hdG9ncsOhZmljbyBiYXNhZG8gZW4gbGEgT2JyYSBvcmlnaW5hbCBkZWwgQXV0b3IgT3JpZ2luYWwiKS4gVGFsIGNyw6lkaXRvIHB1ZWRlIHNlciBpbXBsZW1lbnRhZG8gZGUgY3VhbHF1aWVyIGZvcm1hIHJhem9uYWJsZTsgZW4gZWwgY2Fzbywgc2luIGVtYmFyZ28sIGRlIE9icmFzIERlcml2YWRhcyB1IE9icmFzIENvbGVjdGl2YXMsIHRhbCBjcsOpZGl0byBhcGFyZWNlcsOhLCBjb21vIG3DrW5pbW8sIGRvbmRlIGFwYXJlY2UgZWwgY3LDqWRpdG8gZGUgY3VhbHF1aWVyIG90cm8gYXV0b3IgY29tcGFyYWJsZSB5IGRlIHVuYSBtYW5lcmEsIGFsIG1lbm9zLCB0YW4gZGVzdGFjYWRhIGNvbW8gZWwgY3LDqWRpdG8gZGUgb3RybyBhdXRvciBjb21wYXJhYmxlLiAgCmQuIFBhcmEgZXZpdGFyIHRvZGEgY29uZnVzacOzbiwgZWwgTGljZW5jaWFudGUgYWNsYXJhIHF1ZSwgY3VhbmRvIGxhIG9icmEgZXMgdW5hIGNvbXBvc2ljacOzbiBtdXNpY2FsOgoKaS4gUmVnYWzDrWFzIHBvciBpbnRlcnByZXRhY2nDs24geSBlamVjdWNpw7NuIGJham8gbGljZW5jaWFzIGdlbmVyYWxlcy4gRWwgTGljZW5jaWFudGUgc2UgcmVzZXJ2YSBlbCBkZXJlY2hvIGV4Y2x1c2l2byBkZSBhdXRvcml6YXIgbGEgZWplY3VjacOzbiBww7pibGljYSBvIGxhIGVqZWN1Y2nDs24gcMO6YmxpY2EgZGlnaXRhbCBkZSBsYSBvYnJhIHkgZGUgcmVjb2xlY3Rhciwgc2VhIGluZGl2aWR1YWxtZW50ZSBvIGEgdHJhdsOpcyBkZSB1bmEgc29jaWVkYWQgZGUgZ2VzdGnDs24gY29sZWN0aXZhIGRlIGRlcmVjaG9zIGRlIGF1dG9yIHkgZGVyZWNob3MgY29uZXhvcyAocG9yIGVqZW1wbG8sIFNBWUNPKSwgbGFzIHJlZ2Fsw61hcyBwb3IgbGEgZWplY3VjacOzbiBww7pibGljYSBvIHBvciBsYSBlamVjdWNpw7NuIHDDumJsaWNhIGRpZ2l0YWwgZGUgbGEgb2JyYSAocG9yIGVqZW1wbG8gV2ViY2FzdCkgbGljZW5jaWFkYSBiYWpvIGxpY2VuY2lhcyBnZW5lcmFsZXMsIHNpIGxhIGludGVycHJldGFjacOzbiBvIGVqZWN1Y2nDs24gZGUgbGEgb2JyYSBlc3TDoSBwcmltb3JkaWFsbWVudGUgb3JpZW50YWRhIHBvciBvIGRpcmlnaWRhIGEgbGEgb2J0ZW5jacOzbiBkZSB1bmEgdmVudGFqYSBjb21lcmNpYWwgbyB1bmEgY29tcGVuc2FjacOzbiBtb25ldGFyaWEgcHJpdmFkYS4KaWkuIFJlZ2Fsw61hcyBwb3IgRm9ub2dyYW1hcy4gRWwgTGljZW5jaWFudGUgc2UgcmVzZXJ2YSBlbCBkZXJlY2hvIGV4Y2x1c2l2byBkZSByZWNvbGVjdGFyLCBpbmRpdmlkdWFsbWVudGUgbyBhIHRyYXbDqXMgZGUgdW5hIHNvY2llZGFkIGRlIGdlc3Rpw7NuIGNvbGVjdGl2YSBkZSBkZXJlY2hvcyBkZSBhdXRvciB5IGRlcmVjaG9zIGNvbmV4b3MgKHBvciBlamVtcGxvLCBsb3MgY29uc2FncmFkb3MgcG9yIGxhIFNBWUNPKSwgdW5hIGFnZW5jaWEgZGUgZGVyZWNob3MgbXVzaWNhbGVzIG8gYWxnw7puIGFnZW50ZSBkZXNpZ25hZG8sIGxhcyByZWdhbMOtYXMgcG9yIGN1YWxxdWllciBmb25vZ3JhbWEgcXVlIFVzdGVkIGNyZWUgYSBwYXJ0aXIgZGUgbGEgb2JyYSAo4oCcdmVyc2nDs24gY292ZXLigJ0pIHkgZGlzdHJpYnV5YSwgZW4gbG9zIHTDqXJtaW5vcyBkZWwgcsOpZ2ltZW4gZGUgZGVyZWNob3MgZGUgYXV0b3IsIHNpIGxhIGNyZWFjacOzbiBvIGRpc3RyaWJ1Y2nDs24gZGUgZXNhIHZlcnNpw7NuIGNvdmVyIGVzdMOhIHByaW1vcmRpYWxtZW50ZSBkZXN0aW5hZGEgbyBkaXJpZ2lkYSBhIG9idGVuZXIgdW5hIHZlbnRhamEgY29tZXJjaWFsIG8gdW5hIGNvbXBlbnNhY2nDs24gbW9uZXRhcmlhIHByaXZhZGEuCiAgICAgIAplLiBHZXN0acOzbiBkZSBEZXJlY2hvcyBkZSBBdXRvciBzb2JyZSBJbnRlcnByZXRhY2lvbmVzIHkgRWplY3VjaW9uZXMgRGlnaXRhbGVzIChXZWJDYXN0aW5nKS4gUGFyYSBldml0YXIgdG9kYSBjb25mdXNpw7NuLCBlbCBMaWNlbmNpYW50ZSBhY2xhcmEgcXVlLCBjdWFuZG8gbGEgb2JyYSBzZWEgdW4gZm9ub2dyYW1hLCBlbCBMaWNlbmNpYW50ZSBzZSByZXNlcnZhIGVsIGRlcmVjaG8gZXhjbHVzaXZvIGRlIGF1dG9yaXphciBsYSBlamVjdWNpw7NuIHDDumJsaWNhIGRpZ2l0YWwgZGUgbGEgb2JyYSAocG9yIGVqZW1wbG8sIHdlYmNhc3QpIHkgZGUgcmVjb2xlY3RhciwgaW5kaXZpZHVhbG1lbnRlIG8gYSB0cmF2w6lzIGRlIHVuYSBzb2NpZWRhZCBkZSBnZXN0acOzbiBjb2xlY3RpdmEgZGUgZGVyZWNob3MgZGUgYXV0b3IgeSBkZXJlY2hvcyBjb25leG9zIChwb3IgZWplbXBsbywgQUNJTlBSTyksIGxhcyByZWdhbMOtYXMgcG9yIGxhIGVqZWN1Y2nDs24gcMO6YmxpY2EgZGlnaXRhbCBkZSBsYSBvYnJhIChwb3IgZWplbXBsbywgd2ViY2FzdCksIHN1amV0YSBhIGxhcyBkaXNwb3NpY2lvbmVzIGFwbGljYWJsZXMgZGVsIHLDqWdpbWVuIGRlIERlcmVjaG8gZGUgQXV0b3IsIHNpIGVzdGEgZWplY3VjacOzbiBww7pibGljYSBkaWdpdGFsIGVzdMOhIHByaW1vcmRpYWxtZW50ZSBkaXJpZ2lkYSBhIG9idGVuZXIgdW5hIHZlbnRhamEgY29tZXJjaWFsIG8gdW5hIGNvbXBlbnNhY2nDs24gbW9uZXRhcmlhIHByaXZhZGEuCiAgCjUuIFJlcHJlc2VudGFjaW9uZXMsIEdhcmFudMOtYXMgeSBMaW1pdGFjaW9uZXMgZGUgUmVzcG9uc2FiaWxpZGFkLgpBIE1FTk9TIFFVRSBMQVMgUEFSVEVTIExPIEFDT1JEQVJBTiBERSBPVFJBIEZPUk1BIFBPUiBFU0NSSVRPLCBFTCBMSUNFTkNJQU5URSBPRlJFQ0UgTEEgT0JSQSAoRU4gRUwgRVNUQURPIEVOIEVMIFFVRSBTRSBFTkNVRU5UUkEpIOKAnFRBTCBDVUFM4oCdLCBTSU4gQlJJTkRBUiBHQVJBTlTDjUFTIERFIENMQVNFIEFMR1VOQSBSRVNQRUNUTyBERSBMQSBPQlJBLCBZQSBTRUEgRVhQUkVTQSwgSU1QTMONQ0lUQSwgTEVHQUwgTyBDVUFMUVVJRVJBIE9UUkEsIElOQ0xVWUVORE8sIFNJTiBMSU1JVEFSU0UgQSBFTExBUywgR0FSQU5Uw41BUyBERSBUSVRVTEFSSURBRCwgQ09NRVJDSUFCSUxJREFELCBBREFQVEFCSUxJREFEIE8gQURFQ1VBQ0nDk04gQSBQUk9Qw5NTSVRPIERFVEVSTUlOQURPLCBBVVNFTkNJQSBERSBJTkZSQUNDScOTTiwgREUgQVVTRU5DSUEgREUgREVGRUNUT1MgTEFURU5URVMgTyBERSBPVFJPIFRJUE8sIE8gTEEgUFJFU0VOQ0lBIE8gQVVTRU5DSUEgREUgRVJST1JFUywgU0VBTiBPIE5PIERFU0NVQlJJQkxFUyAoUFVFREFOIE8gTk8gU0VSIEVTVE9TIERFU0NVQklFUlRPUykuIEFMR1VOQVMgSlVSSVNESUNDSU9ORVMgTk8gUEVSTUlURU4gTEEgRVhDTFVTScOTTiBERSBHQVJBTlTDjUFTIElNUEzDjUNJVEFTLCBFTiBDVVlPIENBU08gRVNUQSBFWENMVVNJw5NOIFBVRURFIE5PIEFQTElDQVJTRSBBIFVTVEVELgogIAo2LiBMaW1pdGFjacOzbiBkZSByZXNwb25zYWJpbGlkYWQuCkEgTUVOT1MgUVVFIExPIEVYSUpBIEVYUFJFU0FNRU5URSBMQSBMRVkgQVBMSUNBQkxFLCBFTCBMSUNFTkNJQU5URSBOTyBTRVLDgSBSRVNQT05TQUJMRSBBTlRFIFVTVEVEIFBPUiBEQcORTyBBTEdVTk8sIFNFQSBQT1IgUkVTUE9OU0FCSUxJREFEIEVYVFJBQ09OVFJBQ1RVQUwsIFBSRUNPTlRSQUNUVUFMIE8gQ09OVFJBQ1RVQUwsIE9CSkVUSVZBIE8gU1VCSkVUSVZBLCBTRSBUUkFURSBERSBEQcORT1MgTU9SQUxFUyBPIFBBVFJJTU9OSUFMRVMsIERJUkVDVE9TIE8gSU5ESVJFQ1RPUywgUFJFVklTVE9TIE8gSU1QUkVWSVNUT1MgUFJPRFVDSURPUyBQT1IgRUwgVVNPIERFIEVTVEEgTElDRU5DSUEgTyBERSBMQSBPQlJBLCBBVU4gQ1VBTkRPIEVMIExJQ0VOQ0lBTlRFIEhBWUEgU0lETyBBRFZFUlRJRE8gREUgTEEgUE9TSUJJTElEQUQgREUgRElDSE9TIERBw5FPUy4gQUxHVU5BUyBMRVlFUyBOTyBQRVJNSVRFTiBMQSBFWENMVVNJw5NOIERFIENJRVJUQSBSRVNQT05TQUJJTElEQUQsIEVOIENVWU8gQ0FTTyBFU1RBIEVYQ0xVU0nDk04gUFVFREUgTk8gQVBMSUNBUlNFIEEgVVNURUQuCiAgCjcuIFTDqXJtaW5vLgkKYS4gRXN0YSBMaWNlbmNpYSB5IGxvcyBkZXJlY2hvcyBvdG9yZ2Fkb3MgZW4gdmlydHVkIGRlIGVsbGEgdGVybWluYXLDoW4gYXV0b23DoXRpY2FtZW50ZSBzaSBVc3RlZCBpbmZyaW5nZSBhbGd1bmEgY29uZGljacOzbiBlc3RhYmxlY2lkYSBlbiBlbGxhLiBTaW4gZW1iYXJnbywgbG9zIGluZGl2aWR1b3MgbyBlbnRpZGFkZXMgcXVlIGhhbiByZWNpYmlkbyBPYnJhcyBEZXJpdmFkYXMgbyBDb2xlY3RpdmFzIGRlIFVzdGVkIGRlIGNvbmZvcm1pZGFkIGNvbiBlc3RhIExpY2VuY2lhLCBubyB2ZXLDoW4gdGVybWluYWRhcyBzdXMgbGljZW5jaWFzLCBzaWVtcHJlIHF1ZSBlc3RvcyBpbmRpdmlkdW9zIG8gZW50aWRhZGVzIHNpZ2FuIGN1bXBsaWVuZG8gw61udGVncmFtZW50ZSBsYXMgY29uZGljaW9uZXMgZGUgZXN0YXMgbGljZW5jaWFzLiBMYXMgU2VjY2lvbmVzIDEsIDIsIDUsIDYsIDcsIHkgOCBzdWJzaXN0aXLDoW4gYSBjdWFscXVpZXIgdGVybWluYWNpw7NuIGRlIGVzdGEgTGljZW5jaWEuCmIuIFN1amV0YSBhIGxhcyBjb25kaWNpb25lcyB5IHTDqXJtaW5vcyBhbnRlcmlvcmVzLCBsYSBsaWNlbmNpYSBvdG9yZ2FkYSBhcXXDrSBlcyBwZXJwZXR1YSAoZHVyYW50ZSBlbCBwZXLDrW9kbyBkZSB2aWdlbmNpYSBkZSBsb3MgZGVyZWNob3MgZGUgYXV0b3IgZGUgbGEgb2JyYSkuIE5vIG9ic3RhbnRlIGxvIGFudGVyaW9yLCBlbCBMaWNlbmNpYW50ZSBzZSByZXNlcnZhIGVsIGRlcmVjaG8gYSBwdWJsaWNhciB5L28gZXN0cmVuYXIgbGEgT2JyYSBiYWpvIGNvbmRpY2lvbmVzIGRlIGxpY2VuY2lhIGRpZmVyZW50ZXMgbyBhIGRlamFyIGRlIGRpc3RyaWJ1aXJsYSBlbiBsb3MgdMOpcm1pbm9zIGRlIGVzdGEgTGljZW5jaWEgZW4gY3VhbHF1aWVyIG1vbWVudG87IGVuIGVsIGVudGVuZGlkbywgc2luIGVtYmFyZ28sIHF1ZSBlc2EgZWxlY2Npw7NuIG5vIHNlcnZpcsOhIHBhcmEgcmV2b2NhciBlc3RhIGxpY2VuY2lhIG8gcXVlIGRlYmEgc2VyIG90b3JnYWRhICwgYmFqbyBsb3MgdMOpcm1pbm9zIGRlIGVzdGEgbGljZW5jaWEpLCB5IGVzdGEgbGljZW5jaWEgY29udGludWFyw6EgZW4gcGxlbm8gdmlnb3IgeSBlZmVjdG8gYSBtZW5vcyBxdWUgc2VhIHRlcm1pbmFkYSBjb21vIHNlIGV4cHJlc2EgYXRyw6FzLiBMYSBMaWNlbmNpYSByZXZvY2FkYSBjb250aW51YXLDoSBzaWVuZG8gcGxlbmFtZW50ZSB2aWdlbnRlIHkgZWZlY3RpdmEgc2kgbm8gc2UgbGUgZGEgdMOpcm1pbm8gZW4gbGFzIGNvbmRpY2lvbmVzIGluZGljYWRhcyBhbnRlcmlvcm1lbnRlLgogIAo4LiBWYXJpb3MuCmEuIENhZGEgdmV6IHF1ZSBVc3RlZCBkaXN0cmlidXlhIG8gcG9uZ2EgYSBkaXNwb3NpY2nDs24gcMO6YmxpY2EgbGEgT2JyYSBvIHVuYSBPYnJhIENvbGVjdGl2YSwgZWwgTGljZW5jaWFudGUgb2ZyZWNlcsOhIGFsIGRlc3RpbmF0YXJpbyB1bmEgbGljZW5jaWEgZW4gbG9zIG1pc21vcyB0w6lybWlub3MgeSBjb25kaWNpb25lcyBxdWUgbGEgbGljZW5jaWEgb3RvcmdhZGEgYSBVc3RlZCBiYWpvIGVzdGEgTGljZW5jaWEuCmIuIFNpIGFsZ3VuYSBkaXNwb3NpY2nDs24gZGUgZXN0YSBMaWNlbmNpYSByZXN1bHRhIGludmFsaWRhZGEgbyBubyBleGlnaWJsZSwgc2Vnw7puIGxhIGxlZ2lzbGFjacOzbiB2aWdlbnRlLCBlc3RvIG5vIGFmZWN0YXLDoSBuaSBsYSB2YWxpZGV6IG5pIGxhIGFwbGljYWJpbGlkYWQgZGVsIHJlc3RvIGRlIGNvbmRpY2lvbmVzIGRlIGVzdGEgTGljZW5jaWEgeSwgc2luIGFjY2nDs24gYWRpY2lvbmFsIHBvciBwYXJ0ZSBkZSBsb3Mgc3VqZXRvcyBkZSBlc3RlIGFjdWVyZG8sIGFxdcOpbGxhIHNlIGVudGVuZGVyw6EgcmVmb3JtYWRhIGxvIG3DrW5pbW8gbmVjZXNhcmlvIHBhcmEgaGFjZXIgcXVlIGRpY2hhIGRpc3Bvc2ljacOzbiBzZWEgdsOhbGlkYSB5IGV4aWdpYmxlLgpjLiBOaW5nw7puIHTDqXJtaW5vIG8gZGlzcG9zaWNpw7NuIGRlIGVzdGEgTGljZW5jaWEgc2UgZXN0aW1hcsOhIHJlbnVuY2lhZGEgeSBuaW5ndW5hIHZpb2xhY2nDs24gZGUgZWxsYSBzZXLDoSBjb25zZW50aWRhIGEgbWVub3MgcXVlIGVzYSByZW51bmNpYSBvIGNvbnNlbnRpbWllbnRvIHNlYSBvdG9yZ2FkbyBwb3IgZXNjcml0byB5IGZpcm1hZG8gcG9yIGxhIHBhcnRlIHF1ZSByZW51bmNpZSBvIGNvbnNpZW50YS4KZC4gRXN0YSBMaWNlbmNpYSByZWZsZWphIGVsIGFjdWVyZG8gcGxlbm8gZW50cmUgbGFzIHBhcnRlcyByZXNwZWN0byBhIGxhIE9icmEgYXF1w60gbGljZW5jaWFkYS4gTm8gaGF5IGFycmVnbG9zLCBhY3VlcmRvcyBvIGRlY2xhcmFjaW9uZXMgcmVzcGVjdG8gYSBsYSBPYnJhIHF1ZSBubyBlc3TDqW4gZXNwZWNpZmljYWRvcyBlbiBlc3RlIGRvY3VtZW50by4gRWwgTGljZW5jaWFudGUgbm8gc2UgdmVyw6EgbGltaXRhZG8gcG9yIG5pbmd1bmEgZGlzcG9zaWNpw7NuIGFkaWNpb25hbCBxdWUgcHVlZGEgc3VyZ2lyIGVuIGFsZ3VuYSBjb211bmljYWNpw7NuIGVtYW5hZGEgZGUgVXN0ZWQuIEVzdGEgTGljZW5jaWEgbm8gcHVlZGUgc2VyIG1vZGlmaWNhZGEgc2luIGVsIGNvbnNlbnRpbWllbnRvIG11dHVvIHBvciBlc2NyaXRvIGRlbCBMaWNlbmNpYW50ZSB5IFVzdGVkLgo= 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