Lysozyme plays a dual role against the dimorphic fungus Paracoccidioides brasiliensis

ABSTRACT: : In order to determine the role of lysozyme, an antimicrobial peptide belonging to the innate immune system, against the dimorphic fungus Paracoccidioides brasiliensis, co-cultures of the MH-S murine alveolar macrophages cell line with P. brasiliensis conidia were done; assays to evaluate the effect of physiological and inflammatory concentrations of lysozyme directly on the fungus life cycle were also undertaken. We observed that TNF-α-activated macrophages significantly inhibited the conidia to yeast transition (p = 0.0043) and exerted an important fungicidal effect (p = 0.0044), killing 27% more fungal propagules in comparison with controls. Nonetheless, after adding a selective inhibitor of lysozyme, the fungicidal effect was reverted. When P. brasiliensis propagules were exposed directly to different concentrations of lysozyme, a dual effect was observed. Physiologic concentrations of the enzyme facilitated the conidia-to-yeast transition process (p < 0.05). On the contrary, inflammatory concentrations impaired the normal temperature-dependant fungal transition (p < 0.0001). When yeast cells were exposed to lysozyme, irrespective of concentration, the multiple-budding ability was badly impaired (p < 0.0001). In addition, ultra-structural changes such as subcellular degradation, fusion of lipid vacuoles, lamellar structures and interruption of the fibrilar layer were observed in lysozyme exposed conidia. These results suggest that lysozyme appears to exert a dual role as part of the anti-P. brasiliensis defense mechanisms.

• Autores: Lopera Henao, Damaris Elena; Aristizabal Bernal, Beatriz; Restrepo Moreno, Ángela; Cano Restrepo, Luz Elena; González Marín, Ángel Augusto
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2008
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/29374
• Acceso en línea: http://hdl.handle.net/10495/29374
• Palabra clave: Muramidasa; Muramidase; Paracoccidioides; Macrófagos Alveolares; Macrophages, Alveolar; Factor de Necrosis Tumoral alfa; Tumor Necrosis Factor-alpha; TNF-α; Paracoccidioides brasiliensis
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-sa/2.5/co/