Evidence of two mitochondrial lineages and genetic variability in forensically important Lucilia eximia (Diptera: Calliphoridae) in Colombia
ABSTRACT: Lucilia eximia (Wiedemann, 1819) (Diptera: Calliphoridae) is a blowfly with medical and forensic importance that shows genetic and color variation, however, these variations have not justified the description of new species. But in forensic entomology an accurate identification of species...
- Autores:
-
Pérez Pérez, Juliana
Rúa Uribe, Guillermo León
Pulgarín Díaz, John Alexander
López Rubio, Andrés
Gómez Piñerez, Luz M.
Márquez, Edna J.
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2023
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/45070
- Acceso en línea:
- https://hdl.handle.net/10495/45070
- Palabra clave:
- Entomología Forense
Forensic Entomology
Linaje de la Célula
Cell Lineage
Calliphoridae
COI
https://id.nlm.nih.gov/mesh/D000081230
https://id.nlm.nih.gov/mesh/D019070
https://id.nlm.nih.gov/mesh/D000086542
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc/4.0/
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| dc.title.spa.fl_str_mv |
Evidence of two mitochondrial lineages and genetic variability in forensically important Lucilia eximia (Diptera: Calliphoridae) in Colombia |
| title |
Evidence of two mitochondrial lineages and genetic variability in forensically important Lucilia eximia (Diptera: Calliphoridae) in Colombia |
| spellingShingle |
Evidence of two mitochondrial lineages and genetic variability in forensically important Lucilia eximia (Diptera: Calliphoridae) in Colombia Entomología Forense Forensic Entomology Linaje de la Célula Cell Lineage Calliphoridae COI https://id.nlm.nih.gov/mesh/D000081230 https://id.nlm.nih.gov/mesh/D019070 https://id.nlm.nih.gov/mesh/D000086542 |
| title_short |
Evidence of two mitochondrial lineages and genetic variability in forensically important Lucilia eximia (Diptera: Calliphoridae) in Colombia |
| title_full |
Evidence of two mitochondrial lineages and genetic variability in forensically important Lucilia eximia (Diptera: Calliphoridae) in Colombia |
| title_fullStr |
Evidence of two mitochondrial lineages and genetic variability in forensically important Lucilia eximia (Diptera: Calliphoridae) in Colombia |
| title_full_unstemmed |
Evidence of two mitochondrial lineages and genetic variability in forensically important Lucilia eximia (Diptera: Calliphoridae) in Colombia |
| title_sort |
Evidence of two mitochondrial lineages and genetic variability in forensically important Lucilia eximia (Diptera: Calliphoridae) in Colombia |
| dc.creator.fl_str_mv |
Pérez Pérez, Juliana Rúa Uribe, Guillermo León Pulgarín Díaz, John Alexander López Rubio, Andrés Gómez Piñerez, Luz M. Márquez, Edna J. |
| dc.contributor.author.none.fl_str_mv |
Pérez Pérez, Juliana Rúa Uribe, Guillermo León Pulgarín Díaz, John Alexander López Rubio, Andrés Gómez Piñerez, Luz M. Márquez, Edna J. |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Entomología Médica de la Universidad de Antioquia |
| dc.subject.decs.none.fl_str_mv |
Entomología Forense Forensic Entomology Linaje de la Célula Cell Lineage Calliphoridae |
| topic |
Entomología Forense Forensic Entomology Linaje de la Célula Cell Lineage Calliphoridae COI https://id.nlm.nih.gov/mesh/D000081230 https://id.nlm.nih.gov/mesh/D019070 https://id.nlm.nih.gov/mesh/D000086542 |
| dc.subject.proposal.spa.fl_str_mv |
COI |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D000081230 https://id.nlm.nih.gov/mesh/D019070 https://id.nlm.nih.gov/mesh/D000086542 |
| description |
ABSTRACT: Lucilia eximia (Wiedemann, 1819) (Diptera: Calliphoridae) is a blowfly with medical and forensic importance that shows genetic and color variation, however, these variations have not justified the description of new species. But in forensic entomology an accurate identification of species and subpopulations is crucial. We explored the genetic variation of L. eximia from eight localities, in five natural regions in Colombia using two mitochondrial fragments, including the standard locus for insect identification COI and the Cytb-tRNA-Ser-ND1 region. We found significant differentiation at COI and Cytb-tRNA-Ser-ND1 level, characterizing two lineages and revealing a deep and significant genetic split. High values of FST and genetic distances supported the two lineages. The origin of the divergence of L. eximia remains to discover. Examining whether the lineages have diverse ecological and biological behaviors could be a significant impact on the use of L. eximia in forensic and medical science. Our results could have relevant implications for the use of post-mortem interval estimation based on insect evidence, as well as our sequences improve the database used in DNA-based methods for identifying forensically important flies. |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2025-02-20T02:12:41Z |
| dc.date.available.none.fl_str_mv |
2025-02-20T02:12:41Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Pérez-Pérez J, Pulgarín Díaz JA, López-Rubio A, Gómez-Piñerez LM, Rúa-Uribe G, Márquez EJ. Evidence of two mitochondrial lineages and genetic variability in forensically important Lucilia eximia (Diptera: Calliphoridae) in Colombia. J Med Entomol. 2023 Jul 12;60(4):656-663. doi: 10.1093/jme/tjad031. |
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0022-2585 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/45070 |
| dc.identifier.doi.none.fl_str_mv |
10.1093/jme/tjad031 |
| dc.identifier.eissn.none.fl_str_mv |
1938-2928 |
| identifier_str_mv |
Pérez-Pérez J, Pulgarín Díaz JA, López-Rubio A, Gómez-Piñerez LM, Rúa-Uribe G, Márquez EJ. Evidence of two mitochondrial lineages and genetic variability in forensically important Lucilia eximia (Diptera: Calliphoridae) in Colombia. J Med Entomol. 2023 Jul 12;60(4):656-663. doi: 10.1093/jme/tjad031. 0022-2585 10.1093/jme/tjad031 1938-2928 |
| url |
https://hdl.handle.net/10495/45070 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
J. Med. Entomol. |
| dc.relation.citationendpage.spa.fl_str_mv |
663 |
| dc.relation.citationissue.spa.fl_str_mv |
4 |
| dc.relation.citationstartpage.spa.fl_str_mv |
656 |
| dc.relation.citationvolume.spa.fl_str_mv |
60 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Journal of Medical Entomology |
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https://creativecommons.org/licenses/by-nc/4.0/ |
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http://creativecommons.org/licenses/by/2.5/co/ |
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openAccess |
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8 páginas |
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application/pdf |
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Entomological Society of America |
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Oxford, Inglaterra |
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Universidad de Antioquia |
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Pérez Pérez, JulianaRúa Uribe, Guillermo LeónPulgarín Díaz, John AlexanderLópez Rubio, AndrésGómez Piñerez, Luz M.Márquez, Edna J.Grupo de Entomología Médica de la Universidad de Antioquia2025-02-20T02:12:41Z2025-02-20T02:12:41Z2023Pérez-Pérez J, Pulgarín Díaz JA, López-Rubio A, Gómez-Piñerez LM, Rúa-Uribe G, Márquez EJ. Evidence of two mitochondrial lineages and genetic variability in forensically important Lucilia eximia (Diptera: Calliphoridae) in Colombia. J Med Entomol. 2023 Jul 12;60(4):656-663. doi: 10.1093/jme/tjad031.0022-2585https://hdl.handle.net/10495/4507010.1093/jme/tjad0311938-2928ABSTRACT: Lucilia eximia (Wiedemann, 1819) (Diptera: Calliphoridae) is a blowfly with medical and forensic importance that shows genetic and color variation, however, these variations have not justified the description of new species. But in forensic entomology an accurate identification of species and subpopulations is crucial. We explored the genetic variation of L. eximia from eight localities, in five natural regions in Colombia using two mitochondrial fragments, including the standard locus for insect identification COI and the Cytb-tRNA-Ser-ND1 region. We found significant differentiation at COI and Cytb-tRNA-Ser-ND1 level, characterizing two lineages and revealing a deep and significant genetic split. High values of FST and genetic distances supported the two lineages. The origin of the divergence of L. eximia remains to discover. Examining whether the lineages have diverse ecological and biological behaviors could be a significant impact on the use of L. eximia in forensic and medical science. Our results could have relevant implications for the use of post-mortem interval estimation based on insect evidence, as well as our sequences improve the database used in DNA-based methods for identifying forensically important flies.Colombia. Ministerio de Ciencia, Tecnología e Innovación - MinCienciasTecnológico de Antioquia Institución UniversitariaCOL00081098 páginasapplication/pdfengEntomological Society of AmericaOxford, Inglaterrahttps://creativecommons.org/licenses/by-nc/4.0/http://creativecommons.org/licenses/by/2.5/co/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Evidence of two mitochondrial lineages and genetic variability in forensically important Lucilia eximia (Diptera: Calliphoridae) in ColombiaArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionEntomología ForenseForensic EntomologyLinaje de la CélulaCell LineageCalliphoridaeCOIhttps://id.nlm.nih.gov/mesh/D000081230https://id.nlm.nih.gov/mesh/D019070https://id.nlm.nih.gov/mesh/D000086542J. Med. Entomol.663465660Journal of Medical EntomologyVariabilidad molecular y morfométrica de Lucilia eximia (Wiedemman) (Diptera: Calliphoridae) en ColombiaMinCiencias contracto 80740-836-2020RoR:03fd5ne08RoR:0289gr697PublicationORIGINALPerezJuliana_2023_Evidence_Mitochondrial_Lineages.pdfPerezJuliana_2023_Evidence_Mitochondrial_Lineages.pdfArtículo de investigaciónapplication/pdf5168674https://bibliotecadigital.udea.edu.co/bitstreams/4fea3a4c-15e1-4571-8bdd-ae7dc9c39f24/downloadc37cf817d6a333acef213e64168838f0MD51trueAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/d0f15d00-4a96-49f7-9582-9cdcfe5a7a6f/download8a4605be74aa9ea9d79846c1fba20a33MD52falseAnonymousREADTEXTPerezJuliana_2023_Evidence_Mitochondrial_Lineages.pdf.txtPerezJuliana_2023_Evidence_Mitochondrial_Lineages.pdf.txtExtracted texttext/plain32478https://bibliotecadigital.udea.edu.co/bitstreams/84227608-5cc4-4307-b788-e90348bf57b0/download30344f90cff05a99d0ad1432cf31bbe1MD53falseAnonymousREADTHUMBNAILPerezJuliana_2023_Evidence_Mitochondrial_Lineages.pdf.jpgPerezJuliana_2023_Evidence_Mitochondrial_Lineages.pdf.jpgGenerated Thumbnailimage/jpeg15826https://bibliotecadigital.udea.edu.co/bitstreams/c55fadc9-2321-412e-87cc-fb652910d5e2/download69366e463116ebb9f1d0283e8f4d1509MD54falseAnonymousREAD10495/45070oai:bibliotecadigital.udea.edu.co:10495/450702025-03-26 20:55:11.118https://creativecommons.org/licenses/by-nc/4.0/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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 |