The fermented non-digestible fraction of spent coffee grounds induces apoptosis in human colon cancer cells (SW480)

ABSTRACT: Gut flora-mediated non-digestible/unabsorbed fraction of spent coffee grounds (hgf-NDSCG) was evaluated for its chemopreventive effect and molecular mechanisms involved on human colon adenocar- cinoma SW480 cell survival. Small intestinal digestion released bioavailable ascorbic, chlorogenic and gallic acids from hgf-NDSCG reducing oxidative stress. The hgf-NDSCG inhibited SW480 growth (LC50-19%) dose dependently by decreasing glutathione/oxidized glutathione (GSH/GSSG) ratio, indicat- ing a cellular oxidative stress process. Flow cytometry analysis demonstrated the presence of apoptotic cells in a hypodiploid sub G0/G1-peak as a consequence of partial DNA loss. The pro-apoptotic mechanism of hgf-NDSCG was confirmed by SW480 cell toxicity induced by increased Caspase-3 activity and mitochondria dysfunction modulating oxidative stress using Annexin V and DiOC2(3) staining assays. The chemopreventive effects of spent coffee grounds (SCG) indicate that polyphenols present in hgf-NDSCG, digested and fermented by colon microbiota, can inhibit survival of human colon cancer cell and offer effective prophylactic value to prevent colon cancer.

• Autores: Maldonado Celis, María Elena; García Gutiérrez, Nataly; Rojas López, Mauricio; Loarca Piña, Guadalupe Flavia; Campos Vega, Rocío
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2017
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/36185
• Acceso en línea: https://hdl.handle.net/10495/36185
• Palabra clave: Apoptosis; Puntos de Control del Ciclo Celular; Cell Cycle Checkpoints; Antioxidantes; Antioxidants; Neoplasias del Colon; Colonic Neoplasms; Adenocarcinoma; Adenocarcinoma
• Rights: openAccess
• License: https://creativecommons.org/licenses/by-nc-nd/4.0/