Efecto del all-trans ácido retinoico en la producción de citocinas por células mononucleares de sangre periférica

ABSTRACT: To compare the effect of all-trans retinoic acid in the production of cytokines by peripheral blood mononuclear cells (PBMC) among children with moderate malnutrition (Dn-M), severe (Dn-G) and a control group of children eutrophic (Eu). Methodology: Quasi-experimental research in vitro, to assess the effect of different doses of atRA (0, 17, 30 and 50 nM) on the concentration of cytokines (L-8, IL-1β, IL-6, IL-10, TNF-α and IL-12p70) in PBMC of 21 children, divided into three groups: seven with moderate malnutrition (Dn-M), 7 with severe malnutrition (Dn-G) and 7 healthy children (Eu). Results: Although 71% of malnourished children (Dn-G and Dn-M) showed signs of infection, had a population of leukocytes and lymphocytes lower than the Eu. Children Dn-G reported a concentration of C-reactive protein (CRP), significantly higher than the other two groups and simultaneously younger, shorter (T/E) and lower hemoglobin. The concentration of IL-1β, IL-6, IL-8, TNFα and IL-10 in PBMC was higher in Dn-M than in Dn-G and Eu. Comparison between groups was found that 50 nM of atRA concentration of IL-8 was significantly lower and the higher IL-10 in Dn-G compared with the other two groups. MANOVA analyses indicate these differences were attributed to the fact to belonging the group (Dn-G) and not the dose of atRA (50 nM). Analysis of the effect of different doses of atRA in each group showed a significant decrease with 50nM of atRA of IL-8 in Dn-G and IL-10 in Eu. IL-12p70 increased with doses of 17 nM in Dn-M. Conclusion: atRA affects the production of cytokines but the main determinant of concentration is the type of malnutrition.

• Autores: Deossa Restrepo, Gloria Cecilia; Velásquez Rodríguez, Claudia María; Orozco Cano, Juliana María; González Marín, Ángel Augusto
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2010
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: spa
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/11394
• Acceso en línea: http://hdl.handle.net/10495/11394
• Palabra clave: Ácido retinoico; Retinoic acid; Citocinas; Cytokines; Niños; Children; Desnutrición infantil; Child malnutrition; Desnutrición proteico-calórica; Protein-calorie malnutrition; Proteína C-reactiva; C-reactive protein; Tretinoína; Tretinoin
• Rights: openAccess
• License: https://creativecommons.org/licenses/by-nc-sa/4.0/