A Thermodynamic Study on the Interaction between RH-23 Peptide and DMPC-Based Biomembrane Models
ABSTRACT: Investigation of the interaction between drugs and biomembrane models, as a strategy to study and eventually improve drug/substrate interactions, is a crucial factor in preliminary screening. Synthesized peptides represent a source of potential anticancer and theragnostic drugs. In this st...
- Autores:
-
Giordani Giordani, Cristiano
Russo, Stefano
Torrisi, Cristina
Morante, Silvia
Castelli, Francesco
Grazia Sarpietro, Maria
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2022
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/39538
- Acceso en línea:
- https://hdl.handle.net/10495/39538
- Palabra clave:
- Rastreo Diferencial de Calorimetría
Calorimetry, Differential Scanning
Dimiristoilfosfatidilcolina
Dimyristoylphosphatidylcholine
Péptidos
Peptides
Anticarcinógenos
Anticarcinogenic Agents
https://id.nlm.nih.gov/mesh/D002152
https://id.nlm.nih.gov/mesh/D004134
https://id.nlm.nih.gov/mesh/D010455
https://id.nlm.nih.gov/mesh/D016588
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
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| dc.title.spa.fl_str_mv |
A Thermodynamic Study on the Interaction between RH-23 Peptide and DMPC-Based Biomembrane Models |
| title |
A Thermodynamic Study on the Interaction between RH-23 Peptide and DMPC-Based Biomembrane Models |
| spellingShingle |
A Thermodynamic Study on the Interaction between RH-23 Peptide and DMPC-Based Biomembrane Models Rastreo Diferencial de Calorimetría Calorimetry, Differential Scanning Dimiristoilfosfatidilcolina Dimyristoylphosphatidylcholine Péptidos Peptides Anticarcinógenos Anticarcinogenic Agents https://id.nlm.nih.gov/mesh/D002152 https://id.nlm.nih.gov/mesh/D004134 https://id.nlm.nih.gov/mesh/D010455 https://id.nlm.nih.gov/mesh/D016588 |
| title_short |
A Thermodynamic Study on the Interaction between RH-23 Peptide and DMPC-Based Biomembrane Models |
| title_full |
A Thermodynamic Study on the Interaction between RH-23 Peptide and DMPC-Based Biomembrane Models |
| title_fullStr |
A Thermodynamic Study on the Interaction between RH-23 Peptide and DMPC-Based Biomembrane Models |
| title_full_unstemmed |
A Thermodynamic Study on the Interaction between RH-23 Peptide and DMPC-Based Biomembrane Models |
| title_sort |
A Thermodynamic Study on the Interaction between RH-23 Peptide and DMPC-Based Biomembrane Models |
| dc.creator.fl_str_mv |
Giordani Giordani, Cristiano Russo, Stefano Torrisi, Cristina Morante, Silvia Castelli, Francesco Grazia Sarpietro, Maria |
| dc.contributor.author.none.fl_str_mv |
Giordani Giordani, Cristiano Russo, Stefano Torrisi, Cristina Morante, Silvia Castelli, Francesco Grazia Sarpietro, Maria |
| dc.contributor.researchgroup.spa.fl_str_mv |
Productos Naturales Marinos |
| dc.subject.decs.none.fl_str_mv |
Rastreo Diferencial de Calorimetría Calorimetry, Differential Scanning Dimiristoilfosfatidilcolina Dimyristoylphosphatidylcholine Péptidos Peptides Anticarcinógenos Anticarcinogenic Agents |
| topic |
Rastreo Diferencial de Calorimetría Calorimetry, Differential Scanning Dimiristoilfosfatidilcolina Dimyristoylphosphatidylcholine Péptidos Peptides Anticarcinógenos Anticarcinogenic Agents https://id.nlm.nih.gov/mesh/D002152 https://id.nlm.nih.gov/mesh/D004134 https://id.nlm.nih.gov/mesh/D010455 https://id.nlm.nih.gov/mesh/D016588 |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D002152 https://id.nlm.nih.gov/mesh/D004134 https://id.nlm.nih.gov/mesh/D010455 https://id.nlm.nih.gov/mesh/D016588 |
| description |
ABSTRACT: Investigation of the interaction between drugs and biomembrane models, as a strategy to study and eventually improve drug/substrate interactions, is a crucial factor in preliminary screening. Synthesized peptides represent a source of potential anticancer and theragnostic drugs. In this study, we investigated the interaction of a novel synthesized peptide, called RH-23, with a simplified dimyristoylphosphatidylcholine (DMPC) model of the cellular membrane. The interaction of RH-23 with DMPC, organized either in multilamellar vesicles (MLVs) and in Langmuir-Blodgett (LB) monolayers, was assessed using thermodynamic techniques, namely differential scanning calorimetry (DSC) and LB. The calorimetric evaluations showed that RH-23 inserted into MLVs, causing a stabilization of the phospholipid gel phase that increased with the molar fraction of RH-23. Interplay with LB monolayers revealed that RH-23 interacted with DMPC molecules. This work represents the first experimental thermodynamic study on the interaction between RH-23 and a simplified model of the lipid membrane, thus providing a basis for further evaluations of the effect of RH-23 on biological membranes and its therapeutic/diagnostic potential. |
| publishDate |
2022 |
| dc.date.issued.none.fl_str_mv |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2024-06-01T21:07:14Z |
| dc.date.available.none.fl_str_mv |
2024-06-01T21:07:14Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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Giordani, C.; Russo, S.; Torrisi, C.; Morante, S.; Castelli, F.; Sarpietro, M.G. A Thermodynamic Study on the Interaction between RH-23 Peptide and DMPC-Based Biomembrane Models. Membranes 2022, 12, 1282. https://doi.org/10.3390/10.3390/membranes12121282 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/39538 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/membranes12121282 |
| dc.identifier.eissn.none.fl_str_mv |
2077-0375 |
| identifier_str_mv |
Giordani, C.; Russo, S.; Torrisi, C.; Morante, S.; Castelli, F.; Sarpietro, M.G. A Thermodynamic Study on the Interaction between RH-23 Peptide and DMPC-Based Biomembrane Models. Membranes 2022, 12, 1282. https://doi.org/10.3390/10.3390/membranes12121282 10.3390/membranes12121282 2077-0375 |
| url |
https://hdl.handle.net/10495/39538 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Membranes |
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14 |
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12 |
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1 |
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12 |
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Membranes |
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http://creativecommons.org/licenses/by/2.5/co/ |
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https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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openAccess |
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14 páginas |
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MDPI |
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Giordani Giordani, CristianoRusso, StefanoTorrisi, CristinaMorante, SilviaCastelli, FrancescoGrazia Sarpietro, MariaProductos Naturales Marinos2024-06-01T21:07:14Z2024-06-01T21:07:14Z2022Giordani, C.; Russo, S.; Torrisi, C.; Morante, S.; Castelli, F.; Sarpietro, M.G. A Thermodynamic Study on the Interaction between RH-23 Peptide and DMPC-Based Biomembrane Models. Membranes 2022, 12, 1282. https://doi.org/10.3390/10.3390/membranes12121282https://hdl.handle.net/10495/3953810.3390/membranes121212822077-0375ABSTRACT: Investigation of the interaction between drugs and biomembrane models, as a strategy to study and eventually improve drug/substrate interactions, is a crucial factor in preliminary screening. Synthesized peptides represent a source of potential anticancer and theragnostic drugs. In this study, we investigated the interaction of a novel synthesized peptide, called RH-23, with a simplified dimyristoylphosphatidylcholine (DMPC) model of the cellular membrane. The interaction of RH-23 with DMPC, organized either in multilamellar vesicles (MLVs) and in Langmuir-Blodgett (LB) monolayers, was assessed using thermodynamic techniques, namely differential scanning calorimetry (DSC) and LB. The calorimetric evaluations showed that RH-23 inserted into MLVs, causing a stabilization of the phospholipid gel phase that increased with the molar fraction of RH-23. Interplay with LB monolayers revealed that RH-23 interacted with DMPC molecules. This work represents the first experimental thermodynamic study on the interaction between RH-23 and a simplified model of the lipid membrane, thus providing a basis for further evaluations of the effect of RH-23 on biological membranes and its therapeutic/diagnostic potential.Universidad de Antioquia. 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