A Thermodynamic Study on the Interaction between RH-23 Peptide and DMPC-Based Biomembrane Models

ABSTRACT: Investigation of the interaction between drugs and biomembrane models, as a strategy to study and eventually improve drug/substrate interactions, is a crucial factor in preliminary screening. Synthesized peptides represent a source of potential anticancer and theragnostic drugs. In this study, we investigated the interaction of a novel synthesized peptide, called RH-23, with a simplified dimyristoylphosphatidylcholine (DMPC) model of the cellular membrane. The interaction of RH-23 with DMPC, organized either in multilamellar vesicles (MLVs) and in Langmuir-Blodgett (LB) monolayers, was assessed using thermodynamic techniques, namely differential scanning calorimetry (DSC) and LB. The calorimetric evaluations showed that RH-23 inserted into MLVs, causing a stabilization of the phospholipid gel phase that increased with the molar fraction of RH-23. Interplay with LB monolayers revealed that RH-23 interacted with DMPC molecules. This work represents the first experimental thermodynamic study on the interaction between RH-23 and a simplified model of the lipid membrane, thus providing a basis for further evaluations of the effect of RH-23 on biological membranes and its therapeutic/diagnostic potential.

• Autores: Giordani Giordani, Cristiano; Russo, Stefano; Torrisi, Cristina; Morante, Silvia; Castelli, Francesco; Grazia Sarpietro, Maria
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2022
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/39538
• Acceso en línea: https://hdl.handle.net/10495/39538
• Palabra clave: Rastreo Diferencial de Calorimetría; Calorimetry, Differential Scanning; Dimiristoilfosfatidilcolina; Dimyristoylphosphatidylcholine; Péptidos; Peptides; Anticarcinógenos; Anticarcinogenic Agents; https://id.nlm.nih.gov/mesh/D002152; https://id.nlm.nih.gov/mesh/D004134; https://id.nlm.nih.gov/mesh/D010455; https://id.nlm.nih.gov/mesh/D016588
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/2.5/co/