Sex identification of neotropical macaws (Ara spp.) from invasive and non-invasive samples

ABSTRACT: While amplification of the Chromo-Helicase-DNA-binding gene (CHD) from blood and feathers has been used to sex macaws, the performance of non-invasive samples has been poorly explored. We optimized a comprehensive protocol of molecular sexing of macaws (Ara spp.) from blood, plucked feathe...

Full description

Autores:
Franco Gutiérrez, Lady Johana
Soto Calderón, Iván Darío
Álvarez Cardona, Jóhnatan
Tipo de recurso:
Article of investigation
Fecha de publicación:
2017
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/34493
Acceso en línea:
https://hdl.handle.net/10495/34493
https://asociacioncolombianadeornitologia.org/ojs/index.php/roc/article/view/389
Palabra clave:
Parrots
Loros
Sex Chromosomes
Cromosomas Sexuales
Muestreo
Sampling
Gen CHD
CHD gene
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc/2.5/co/
id UDEA2_c8f5046a46d4e7e10dee6872ead23bf8
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/34493
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Sex identification of neotropical macaws (Ara spp.) from invasive and non-invasive samples
dc.title.translated.spa.fl_str_mv identificación del sexo en guacamayas neotropicales (Ara spp) a partir de muestras invasivas y no invasivas
title Sex identification of neotropical macaws (Ara spp.) from invasive and non-invasive samples
spellingShingle Sex identification of neotropical macaws (Ara spp.) from invasive and non-invasive samples
Parrots
Loros
Sex Chromosomes
Cromosomas Sexuales
Muestreo
Sampling
Gen CHD
CHD gene
title_short Sex identification of neotropical macaws (Ara spp.) from invasive and non-invasive samples
title_full Sex identification of neotropical macaws (Ara spp.) from invasive and non-invasive samples
title_fullStr Sex identification of neotropical macaws (Ara spp.) from invasive and non-invasive samples
title_full_unstemmed Sex identification of neotropical macaws (Ara spp.) from invasive and non-invasive samples
title_sort Sex identification of neotropical macaws (Ara spp.) from invasive and non-invasive samples
dc.creator.fl_str_mv Franco Gutiérrez, Lady Johana
Soto Calderón, Iván Darío
Álvarez Cardona, Jóhnatan
dc.contributor.author.none.fl_str_mv Franco Gutiérrez, Lady Johana
Soto Calderón, Iván Darío
Álvarez Cardona, Jóhnatan
dc.contributor.researchgroup.spa.fl_str_mv Genética Molecular (GENMOL)
dc.subject.decs.none.fl_str_mv Parrots
Loros
Sex Chromosomes
Cromosomas Sexuales
topic Parrots
Loros
Sex Chromosomes
Cromosomas Sexuales
Muestreo
Sampling
Gen CHD
CHD gene
dc.subject.lemb.none.fl_str_mv Muestreo
Sampling
dc.subject.proposal.spa.fl_str_mv Gen CHD
CHD gene
description ABSTRACT: While amplification of the Chromo-Helicase-DNA-binding gene (CHD) from blood and feathers has been used to sex macaws, the performance of non-invasive samples has been poorly explored. We optimized a comprehensive protocol of molecular sexing of macaws (Ara spp.) from blood, plucked feathers and fecal samples, using the 2550F/2718R primers to amplify the CHD gene. This protocol is versatile and useful for studies in both ex situ and in situ settings. We successfully identified the sex of macaws from the three types of tissue. However, we recommend sexing from fresh fecal pellets to minimize physical restriction, stress and risk of injury on animals.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2023-04-07T02:08:29Z
dc.date.available.none.fl_str_mv 2023-04-07T02:08:29Z
dc.type.spa.fl_str_mv Artículo de investigación
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.coarversion.spa.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_2df8fbb1
status_str publishedVersion
dc.identifier.issn.none.fl_str_mv 1794-0915
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10495/34493
dc.identifier.url.spa.fl_str_mv https://asociacioncolombianadeornitologia.org/ojs/index.php/roc/article/view/389
identifier_str_mv 1794-0915
url https://hdl.handle.net/10495/34493
https://asociacioncolombianadeornitologia.org/ojs/index.php/roc/article/view/389
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Ornitol. Colomb.
dc.relation.citationendpage.spa.fl_str_mv 7
dc.relation.citationissue.spa.fl_str_mv 16
dc.relation.citationstartpage.spa.fl_str_mv 1
dc.relation.ispartofjournal.spa.fl_str_mv Ornitología Colombiana
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc/2.5/co/
dc.rights.uri.spa.fl_str_mv https://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/2.5/co/
https://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.extent.spa.fl_str_mv 7
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Asociación Colombiana de Ornitología
dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
institution Universidad de Antioquia
bitstream.url.fl_str_mv https://bibliotecadigital.udea.edu.co/bitstreams/91fbbc92-c23d-4db9-ab93-be958de78299/download
https://bibliotecadigital.udea.edu.co/bitstreams/2795f170-41e7-422a-b610-2c7a2d252f4c/download
https://bibliotecadigital.udea.edu.co/bitstreams/00a44b14-b1bf-4d4e-adb9-ff019bfcd73b/download
https://bibliotecadigital.udea.edu.co/bitstreams/48b2fc40-b155-4bc5-8b4e-16fe7afc6bb4/download
https://bibliotecadigital.udea.edu.co/bitstreams/e7450401-d7b2-4130-8c35-8ae15b0baa81/download
bitstream.checksum.fl_str_mv b0e80cfe73c812e797ab3bd5d2b76f99
c0c92b0ffc8b7d22d9cf56754a416a76
8a4605be74aa9ea9d79846c1fba20a33
c75b404147d9a90cfd2bdec62cc74681
0fa613326d73ecdd69c2451f376e4ea8
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional de la Universidad de Antioquia
repository.mail.fl_str_mv aplicacionbibliotecadigitalbiblioteca@udea.edu.co
_version_ 1851052259839836160
spelling Franco Gutiérrez, Lady JohanaSoto Calderón, Iván DaríoÁlvarez Cardona, JóhnatanGenética Molecular (GENMOL)2023-04-07T02:08:29Z2023-04-07T02:08:29Z20171794-0915https://hdl.handle.net/10495/34493https://asociacioncolombianadeornitologia.org/ojs/index.php/roc/article/view/389ABSTRACT: While amplification of the Chromo-Helicase-DNA-binding gene (CHD) from blood and feathers has been used to sex macaws, the performance of non-invasive samples has been poorly explored. We optimized a comprehensive protocol of molecular sexing of macaws (Ara spp.) from blood, plucked feathers and fecal samples, using the 2550F/2718R primers to amplify the CHD gene. This protocol is versatile and useful for studies in both ex situ and in situ settings. We successfully identified the sex of macaws from the three types of tissue. However, we recommend sexing from fresh fecal pellets to minimize physical restriction, stress and risk of injury on animals.COL00067237application/pdfengAsociación Colombiana de OrnitologíaBogotá, Colombiahttp://creativecommons.org/licenses/by-nc/2.5/co/https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Sex identification of neotropical macaws (Ara spp.) from invasive and non-invasive samplesidentificación del sexo en guacamayas neotropicales (Ara spp) a partir de muestras invasivas y no invasivasArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionParrotsLorosSex ChromosomesCromosomas SexualesMuestreoSamplingGen CHDCHD geneOrnitol. Colomb.7161Ornitología ColombianaPublicationORIGINALSotoIvan_2017_SexNeotropicalMacaws.pdfSotoIvan_2017_SexNeotropicalMacaws.pdfArtículo de investigaciónapplication/pdf792884https://bibliotecadigital.udea.edu.co/bitstreams/91fbbc92-c23d-4db9-ab93-be958de78299/downloadb0e80cfe73c812e797ab3bd5d2b76f99MD51trueAnonymousREADCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8933https://bibliotecadigital.udea.edu.co/bitstreams/2795f170-41e7-422a-b610-2c7a2d252f4c/downloadc0c92b0ffc8b7d22d9cf56754a416a76MD52falseAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/00a44b14-b1bf-4d4e-adb9-ff019bfcd73b/download8a4605be74aa9ea9d79846c1fba20a33MD53falseAnonymousREADTEXTSotoIvan_2017_SexNeotropicalMacaws.pdf.txtSotoIvan_2017_SexNeotropicalMacaws.pdf.txtExtracted texttext/plain21428https://bibliotecadigital.udea.edu.co/bitstreams/48b2fc40-b155-4bc5-8b4e-16fe7afc6bb4/downloadc75b404147d9a90cfd2bdec62cc74681MD54falseAnonymousREADTHUMBNAILSotoIvan_2017_SexNeotropicalMacaws.pdf.jpgSotoIvan_2017_SexNeotropicalMacaws.pdf.jpgGenerated Thumbnailimage/jpeg16561https://bibliotecadigital.udea.edu.co/bitstreams/e7450401-d7b2-4130-8c35-8ae15b0baa81/download0fa613326d73ecdd69c2451f376e4ea8MD55falseAnonymousREAD10495/34493oai:bibliotecadigital.udea.edu.co:10495/344932025-03-26 19:30:11.606http://creativecommons.org/licenses/by-nc/2.5/co/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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

Publicaciones similares