Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean

ABSTRACT:Arboviruses belonging to the genera Flavivirus and Alphavirus were detected in mosquitoes in a rural area of San Bernardo del Viento (Córdoba, Colombia). A total of 22,180 mosquitoes were collected, sorted into 2,102 pools, and tested by generic/nested reverse transcription-polymerase chain...

Full description

Autores:
Gallego Gómez, Juan Carlos
Hoyos López, Richard Onalbi
Rúa Uribe, Guillermo León
Suaza Vasco, Juan
Uribe Soto, Sandra Inés
Tipo de recurso:
Article of investigation
Fecha de publicación:
2016
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/39014
Acceso en línea:
https://hdl.handle.net/10495/39014
Palabra clave:
Alphavirus
Región del Caribe
Caribbean Region
Colombia
Culicidae
Flavivirus
Filogenia
Phylogeny
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Reverse Transcriptase Polymerase Chain Reaction
Población Rural
Rural Population
Enfermedades Transmisibles Emergentes
Communicable Diseases, Emerging
Humedales
Wetlands
https://id.nlm.nih.gov/mesh/D000524
https://id.nlm.nih.gov/mesh/D017691
https://id.nlm.nih.gov/mesh/D003105
https://id.nlm.nih.gov/mesh/D009033
https://id.nlm.nih.gov/mesh/D005416
https://id.nlm.nih.gov/mesh/D010802
https://id.nlm.nih.gov/mesh/D020133
https://id.nlm.nih.gov/mesh/D012424
https://id.nlm.nih.gov/mesh/D021821
https://id.nlm.nih.gov/mesh/D053833
Rights
openAccess
License
https://creativecommons.org/licenses/by/4.0/
id UDEA2_c82b0d8d0a1b3fd2c0be80e05d8dc749
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/39014
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean
title Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean
spellingShingle Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean
Alphavirus
Región del Caribe
Caribbean Region
Colombia
Culicidae
Flavivirus
Filogenia
Phylogeny
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Reverse Transcriptase Polymerase Chain Reaction
Población Rural
Rural Population
Enfermedades Transmisibles Emergentes
Communicable Diseases, Emerging
Humedales
Wetlands
https://id.nlm.nih.gov/mesh/D000524
https://id.nlm.nih.gov/mesh/D017691
https://id.nlm.nih.gov/mesh/D003105
https://id.nlm.nih.gov/mesh/D009033
https://id.nlm.nih.gov/mesh/D005416
https://id.nlm.nih.gov/mesh/D010802
https://id.nlm.nih.gov/mesh/D020133
https://id.nlm.nih.gov/mesh/D012424
https://id.nlm.nih.gov/mesh/D021821
https://id.nlm.nih.gov/mesh/D053833
title_short Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean
title_full Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean
title_fullStr Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean
title_full_unstemmed Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean
title_sort Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean
dc.creator.fl_str_mv Gallego Gómez, Juan Carlos
Hoyos López, Richard Onalbi
Rúa Uribe, Guillermo León
Suaza Vasco, Juan
Uribe Soto, Sandra Inés
dc.contributor.author.none.fl_str_mv Gallego Gómez, Juan Carlos
Hoyos López, Richard Onalbi
Rúa Uribe, Guillermo León
Suaza Vasco, Juan
Uribe Soto, Sandra Inés
dc.contributor.researchgroup.spa.fl_str_mv Grupo de Entomología Médica de la Universidad de Antioquia
Grupo Medicina Molecular y de Translación
dc.subject.decs.none.fl_str_mv Alphavirus
Región del Caribe
Caribbean Region
Colombia
Culicidae
Flavivirus
Filogenia
Phylogeny
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Reverse Transcriptase Polymerase Chain Reaction
Población Rural
Rural Population
Enfermedades Transmisibles Emergentes
Communicable Diseases, Emerging
Humedales
Wetlands
topic Alphavirus
Región del Caribe
Caribbean Region
Colombia
Culicidae
Flavivirus
Filogenia
Phylogeny
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Reverse Transcriptase Polymerase Chain Reaction
Población Rural
Rural Population
Enfermedades Transmisibles Emergentes
Communicable Diseases, Emerging
Humedales
Wetlands
https://id.nlm.nih.gov/mesh/D000524
https://id.nlm.nih.gov/mesh/D017691
https://id.nlm.nih.gov/mesh/D003105
https://id.nlm.nih.gov/mesh/D009033
https://id.nlm.nih.gov/mesh/D005416
https://id.nlm.nih.gov/mesh/D010802
https://id.nlm.nih.gov/mesh/D020133
https://id.nlm.nih.gov/mesh/D012424
https://id.nlm.nih.gov/mesh/D021821
https://id.nlm.nih.gov/mesh/D053833
dc.subject.meshuri.none.fl_str_mv https://id.nlm.nih.gov/mesh/D000524
https://id.nlm.nih.gov/mesh/D017691
https://id.nlm.nih.gov/mesh/D003105
https://id.nlm.nih.gov/mesh/D009033
https://id.nlm.nih.gov/mesh/D005416
https://id.nlm.nih.gov/mesh/D010802
https://id.nlm.nih.gov/mesh/D020133
https://id.nlm.nih.gov/mesh/D012424
https://id.nlm.nih.gov/mesh/D021821
https://id.nlm.nih.gov/mesh/D053833
description ABSTRACT:Arboviruses belonging to the genera Flavivirus and Alphavirus were detected in mosquitoes in a rural area of San Bernardo del Viento (Córdoba, Colombia). A total of 22,180 mosquitoes were collected, sorted into 2,102 pools, and tested by generic/nested reverse transcription-polymerase chain reaction. Venezuelan equine encephalitis virus, dengue virus, West Nile virus, St. Louis encephalitis virus, yellow fever virus, and Culex flavivirus were detected and identified by sequencing. The detection of arboviral pathogens in this zone represents possible circulation and indicates a human health risk, demonstrating the importance of virological surveillance activities.
publishDate 2016
dc.date.issued.none.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2024-04-12T18:42:18Z
dc.date.available.none.fl_str_mv 2024-04-12T18:42:18Z
dc.type.spa.fl_str_mv Artículo de investigación
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.coarversion.spa.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_2df8fbb1
status_str publishedVersion
dc.identifier.citation.spa.fl_str_mv Hoyos-López R, Suaza-Vasco J, Rúa-Uribe G, Uribe S, Gallego-Gómez JC. Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean. Mem Inst Oswaldo Cruz. 2016 Oct;111(10):625-634. doi: 10.1590/0074-02760160096. Epub 2016 Oct 3. PMID: 27706377; PMCID: PMC5066328.
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10495/39014
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760160096
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv Hoyos-López R, Suaza-Vasco J, Rúa-Uribe G, Uribe S, Gallego-Gómez JC. Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean. Mem Inst Oswaldo Cruz. 2016 Oct;111(10):625-634. doi: 10.1590/0074-02760160096. Epub 2016 Oct 3. PMID: 27706377; PMCID: PMC5066328.
0074-0276
10.1590/0074-02760160096
1678-8060
url https://hdl.handle.net/10495/39014
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Mem. Inst. Oswaldo Cruz.
dc.relation.citationendpage.spa.fl_str_mv 634
dc.relation.citationissue.spa.fl_str_mv 10
dc.relation.citationstartpage.spa.fl_str_mv 625
dc.relation.citationvolume.spa.fl_str_mv 111
dc.relation.ispartofjournal.spa.fl_str_mv Memórias do Instituto Oswaldo Cruz
dc.rights.uri.spa.fl_str_mv https://creativecommons.org/licenses/by/4.0/
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/2.5/co/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
http://creativecommons.org/licenses/by/2.5/co/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.extent.spa.fl_str_mv 10 páginas
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Instituto Oswaldo Cruz
dc.publisher.place.spa.fl_str_mv Río de Janeiro, Brasil
institution Universidad de Antioquia
bitstream.url.fl_str_mv https://bibliotecadigital.udea.edu.co/bitstreams/3f55e3f9-131b-4341-9d76-9e69677b077c/download
https://bibliotecadigital.udea.edu.co/bitstreams/ce944c1f-4306-4ff4-8786-872a32e29169/download
https://bibliotecadigital.udea.edu.co/bitstreams/0455203a-ae63-4679-a23c-337a1093ed35/download
https://bibliotecadigital.udea.edu.co/bitstreams/fcb63dbe-8d5b-4e00-86d6-f31c673c41e9/download
https://bibliotecadigital.udea.edu.co/bitstreams/af78c6a0-9367-43fd-9c47-31b01606d428/download
bitstream.checksum.fl_str_mv 1646d1f6b96dbbbc38035efc9239ac9c
a7c61628e7b36e7943f1e47ba2bfc686
8a4605be74aa9ea9d79846c1fba20a33
b457a0475e512fb790e34daa057f4b06
1d45b21b7934be81e760f42a4998dec0
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional de la Universidad de Antioquia
repository.mail.fl_str_mv aplicacionbibliotecadigitalbiblioteca@udea.edu.co
_version_ 1851052167702511616
spelling Gallego Gómez, Juan CarlosHoyos López, Richard OnalbiRúa Uribe, Guillermo LeónSuaza Vasco, JuanUribe Soto, Sandra InésGrupo de Entomología Médica de la Universidad de AntioquiaGrupo Medicina Molecular y de Translación2024-04-12T18:42:18Z2024-04-12T18:42:18Z2016Hoyos-López R, Suaza-Vasco J, Rúa-Uribe G, Uribe S, Gallego-Gómez JC. Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean. Mem Inst Oswaldo Cruz. 2016 Oct;111(10):625-634. doi: 10.1590/0074-02760160096. Epub 2016 Oct 3. PMID: 27706377; PMCID: PMC5066328.0074-0276https://hdl.handle.net/10495/3901410.1590/0074-027601600961678-8060ABSTRACT:Arboviruses belonging to the genera Flavivirus and Alphavirus were detected in mosquitoes in a rural area of San Bernardo del Viento (Córdoba, Colombia). A total of 22,180 mosquitoes were collected, sorted into 2,102 pools, and tested by generic/nested reverse transcription-polymerase chain reaction. Venezuelan equine encephalitis virus, dengue virus, West Nile virus, St. Louis encephalitis virus, yellow fever virus, and Culex flavivirus were detected and identified by sequencing. The detection of arboviral pathogens in this zone represents possible circulation and indicates a human health risk, demonstrating the importance of virological surveillance activities.Colombia. Ministerio de Ciencia, Tecnología e Innovación - MincienciasCOL0008109COL014013910 páginasapplication/pdfengInstituto Oswaldo CruzRío de Janeiro, Brasilhttps://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/2.5/co/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian CaribbeanArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAlphavirusRegión del CaribeCaribbean RegionColombiaCulicidaeFlavivirusFilogeniaPhylogenyReacción en Cadena de la Polimerasa de Transcriptasa InversaReverse Transcriptase Polymerase Chain ReactionPoblación RuralRural PopulationEnfermedades Transmisibles EmergentesCommunicable Diseases, EmergingHumedalesWetlandshttps://id.nlm.nih.gov/mesh/D000524https://id.nlm.nih.gov/mesh/D017691https://id.nlm.nih.gov/mesh/D003105https://id.nlm.nih.gov/mesh/D009033https://id.nlm.nih.gov/mesh/D005416https://id.nlm.nih.gov/mesh/D010802https://id.nlm.nih.gov/mesh/D020133https://id.nlm.nih.gov/mesh/D012424https://id.nlm.nih.gov/mesh/D021821https://id.nlm.nih.gov/mesh/D053833Mem. Inst. Oswaldo Cruz.63410625111Memórias do Instituto Oswaldo Cruz111599326198RoR:03fd5ne08PublicationCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927https://bibliotecadigital.udea.edu.co/bitstreams/3f55e3f9-131b-4341-9d76-9e69677b077c/download1646d1f6b96dbbbc38035efc9239ac9cMD52falseAnonymousREADORIGINALGallegoJuan_2016_Molecular_Flaviviruses_Alphaviruses.pdfGallegoJuan_2016_Molecular_Flaviviruses_Alphaviruses.pdfArtículo de investigaciónapplication/pdf5555622https://bibliotecadigital.udea.edu.co/bitstreams/ce944c1f-4306-4ff4-8786-872a32e29169/downloada7c61628e7b36e7943f1e47ba2bfc686MD51trueAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/0455203a-ae63-4679-a23c-337a1093ed35/download8a4605be74aa9ea9d79846c1fba20a33MD53falseAnonymousREADTEXTGallegoJuan_2016_Molecular_Flaviviruses_Alphaviruses.pdf.txtGallegoJuan_2016_Molecular_Flaviviruses_Alphaviruses.pdf.txtExtracted texttext/plain48196https://bibliotecadigital.udea.edu.co/bitstreams/fcb63dbe-8d5b-4e00-86d6-f31c673c41e9/downloadb457a0475e512fb790e34daa057f4b06MD56falseAnonymousREADTHUMBNAILGallegoJuan_2016_Molecular_Flaviviruses_Alphaviruses.pdf.jpgGallegoJuan_2016_Molecular_Flaviviruses_Alphaviruses.pdf.jpgGenerated Thumbnailimage/jpeg15696https://bibliotecadigital.udea.edu.co/bitstreams/af78c6a0-9367-43fd-9c47-31b01606d428/download1d45b21b7934be81e760f42a4998dec0MD57falseAnonymousREAD10495/39014oai:bibliotecadigital.udea.edu.co:10495/390142025-03-26 17:59:42.44https://creativecommons.org/licenses/by/4.0/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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