Progesterone effects on mouse sperm kinetics in conditions of viscosity

ABSTRACT: The spermatozoa delivered to the female genital tract need to swim towards the oocyte through viscous secretions. Once close to the oocyte, the spermatozoa are guided by a gradient of progesterone (P4) and other unknown chemoattractants via a process known as chemotaxis. Using polyvinylpyrrolidone to establish the conditions of viscosity, we examined the response of mouse spermatozoa to P4. Herein, we show that in low-viscous media, P4 induces hyperactive-like motility whereby sperm show erratic trajectories and non-progressive movement. However, an opposite response is produced in viscous medium in that trajectories are linear and motility is more progressive and less erratic. Our observations provide a behavioural explanation for the chemotaxis of spermatozoa swimming under viscous conditions in a spatial gradient of the chemoattractant P4. They also highlight the importance of using viscous solutions to mimic in vivo conditions when analysing sperm behaviour in response to any stimulus.

• Autores: López Cardona, Angela Patricia; Pérez Cerezales, Serafín; Gutiérrez Adán, Alfonso
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2016
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/37520
• Acceso en línea: https://hdl.handle.net/10495/37520
• Palabra clave: Reacción Acrosómica; Acrosome Reaction; Factores Quimiotácticos - farmacología; Chemotactic Factors - pharmacology; Quimiotaxis - efectos de los fármacos; Chemotaxis - drug effects; Cinética; Kinetics; Espermatozoides - efectos de los fármacos; Spermatozoa - drug effects; Motilidad Espermática - efectos de los fármacos; Sperm Motility - drug effects; Ratones Endogámicos C57BL; Mice, Inbred C57BL; Progesterona; Progesterone; http://id.nlm.nih.gov/mesh/D020101; http://id.nlm.nih.gov/mesh/D000818; http://id.nlm.nih.gov/mesh/D002630; http://id.nlm.nih.gov/mesh/D002633; http://id.nlm.nih.gov/mesh/D005260; http://id.nlm.nih.gov/mesh/D007700; http://id.nlm.nih.gov/mesh/D008810; http://id.nlm.nih.gov/mesh/D011374; http://id.nlm.nih.gov/mesh/D013081; http://id.nlm.nih.gov/mesh/D013094
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/2.5/co/