Genotoxicity and cytotoxicity of three microcystin-LR containing cyanobacterial samples from Antioquia, Colombia

ABSTRACT: The presence of cyanobacterial blooms and cyanotoxins in water presents a global problem due to the deterioration of ecosystems and the possibility of poisoning in human and animals. Microcystin LR is the most widely distributed cyanotoxin and liver cells are its main target. In the present study, HepG2 cells were used to determine DNA damage of three crude extracts of cyanobacterial blooms containing MC-LR, through comet assay. The results show that all extracts at a concentration of 500 μg mL−1 caused low damage in hepatocytes exposed for 24 h, but produced total mortality even at low concentrations at 48 h. Moreover, balloons corresponding to cell apoptosis were found. Through HPLC/MS, MC-LR was detected in all samples of cyanobacterial blooms at concentrations of (5,65 μg ml−1) in sample 1, (1,24 μg ml−1) in sample 2 and (57,29 μg ml−1) in sample 3. In addition, in all samples high molecular weights peaks were detected, that may correspond to other microcystins. Besides, the cytotoxic effect of a cyanobacterial bloom and some of its chromatographic fractions from the crude extracts were evaluated in U-937, J774, Hela and Vero cell lines, using the enzymatic micromethod (MTT). The highest toxicity was detected in U-937 cells (LC50 = 29.7 μg mL−1) and Vero cells (LC50 = 39.7 μg mL-1). Based on these results, it is important to remark that genotoxic and cytotoxicity assays are valuable methods to predict potential biological risks in waters contaminated with blooms of cyanobacteria, since chemical analysis can only describe the presence of cyanotoxins, but not their biological effects.

• Autores: Herrera Loaiza, Natalia Andrea; Robledo Restrepo, Sara María; Ortiz Trujillo, Isabel Cristina; Echeverri López, Luis Fernando; Herrera Mazo, Carolina; Orozco Jiménez, Luz Yaneth; Agudelo Echavarría, Diana María
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2018
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/43184
• Acceso en línea: https://hdl.handle.net/10495/43184
• Palabra clave: Cianobacterias; Cyanobacteria; Compuestos de Bencilo; Benzyl Compounds; Células Cultivadas; Cells, Cultured; Chlorocebus aethiops; Colombia; Ensayo Cometa; Daño del ADN; DNA Damage; Monitoreo del Ambiente; Environmental Monitoring; Células Hep G2; Hep G2 Cells; Toxinas Marinas; Marine Toxins; Microcistinas; Microcystins; Pruebas de Mutagenicidad - métodos; Mutagenicity Tests - methods; Pirazinas; Pyrazines; Células U937; U937 Cells; Células Vero; Vero Cells; Genotoxicidad; Genotoxicity; http://aims.fao.org/aos/agrovoc/c_36150; https://id.nlm.nih.gov/mesh/D000458; https://id.nlm.nih.gov/mesh/D001593; https://id.nlm.nih.gov/mesh/D002478; https://id.nlm.nih.gov/mesh/D002522; https://id.nlm.nih.gov/mesh/D003105; Comet Assay; https://id.nlm.nih.gov/mesh/D020552; https://id.nlm.nih.gov/mesh/D004249; https://id.nlm.nih.gov/mesh/D004784; https://id.nlm.nih.gov/mesh/D056945; https://id.nlm.nih.gov/mesh/D008387; https://id.nlm.nih.gov/mesh/D052998; https://id.nlm.nih.gov/mesh/D009152; https://id.nlm.nih.gov/mesh/D011719; https://id.nlm.nih.gov/mesh/D020298; https://id.nlm.nih.gov/mesh/D014709
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-nd/2.5/co/