Detección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae)

ABSTRACT: This study reports the detection, by biochemical methods, of extracellular enzymes related to the biological activity of nine strains of the complex Metarhizium anisopliae (Metsch.) Sorokin. We determined the pathogenic capacity against the insect Cylas formicarius Fabricius at different concentrations, calculated the lethal time and concentration, and analyzed both the presence of extracellular enzymes in the fungal strains as well as their relation to the entomopathogenic effect and virulence against C. formicarius. All strains of the M. anisopliae complex studied were pathogenic with an enzymatic activity index greater than 1. The LBM-30 strain was the most effective with presence of protease, chitinase, caseinase, amylase and lipase enzymes. This result corroborates the relationship between of the enzymatic activity with the observed virulence.

• Autores: Gato Cárdenas, Yohana; Márquez Gutiérrez, María Elena; Baró Robaina, Yamilé; Calle Osorno, Jaime de Jesús
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2017
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: spa
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/10170
• Acceso en línea: http://hdl.handle.net/10495/10170
• Palabra clave: Virulencia; Virulence; Control biológico; Biological control; Hongos entomopatógenos; Entomopathogenic fungi; Actividad enzimática; Enzyme activity; Coleóptera; Coleoptera; Actividad patogénica; http://aims.fao.org/aos/agrovoc/c_2604; http://aims.fao.org/aos/agrovoc/c_1747
• Rights: openAccess
• License: https://creativecommons.org/licenses/by-nc-sa/4.0/