Environmental Factors Modulate the Role of orf21 Sigma Factor in Clavulanic Acid Production in Streptomyces Clavuligerus ATCC27064

Sigma factors and sigma factor-related mechanisms control antibiotic production in Strep- tomyces. In this contribution, the orf21 gene was overexpressed in the wild-type strain of Strepto-myces clavuligerus ATCC2764, yielding S. clavuligerus/pIORF21, to further evaluate its regulatory effect on clavulanic acid (CA) biosynthesis under different culture medium conditions. The orf21 overexpression, regulated under the constitutive promoter ermE*, led to 2.6-fold increase in CA production in GSPG medium, and a 1.8-fold decrease using ISP medium. As for GYM and MYM media, S. clavuligerus/pIORF21 strain showed higher aerial mycelium production compared to control. Glycerol uptake rate profile was affected by orf21 overexpression. Furthermore, in GSPG, S. clavuligerus/pIORF21 slightly increased the expression of adpA and gcas genes, whilst, in ISP, the claR gene expression was drastically reduced, which is consistent with a decreased CA production, observed in this medium. These findings suggest the protein encoded by the orf21 gene plays a role in the regulation of CA biosynthesis as a response to the nutritional composition of the medium.

• Autores: Pinilla Mendoza, Laura Inés; Ríos Estepa, Rigoberto; Toro Navarro, León Felipe; Aguirre Hoyos, Vanessa Yuliet; Patiño Cervantes, Luisa Fernanda
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2022
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/45950
• Acceso en línea: https://hdl.handle.net/10495/45950
• Palabra clave: Ácido Clavulánico; Clavulanic Acid; Expresión Génica; Gene Expression; Streptomyces; Antibacterianos; Anti-Bacterial Agents; Factor sigma; Sigma Factor; Streptomyces clavuligerus; https://id.nlm.nih.gov/mesh/D019818; https://id.nlm.nih.gov/mesh/D015870; https://id.nlm.nih.gov/mesh/D013302; https://id.nlm.nih.gov/mesh/D000900; https://id.nlm.nih.gov/mesh/D012808; ODS 3: Salud y bienestar. Garantizar una vida sana y promover el bienestar de todos a todas las edades; ODS 12: Producción y consumo responsables. Garantizar modalidades de consumo y producción sostenibles
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/4.0/