Gene expression analysis of Paracoccidioides brasiliensis transition from conidium to yeast

ABSTRACT: Paracoccidioides brasiliensis infectious process relies on the initial expression of virulence faactors that are assumed to be controlled by molecular mechanisms through which the conidia and/or mycelial fragments convert to yeast cells. In order to analyze the profile of the thermally-induced dimorphic gene expression, 48 h C-L transition cultures which had been incubated at 36°C were studied. By this time approximately 50% of the conidial population had already reverted to yeast form cells. At this transition time, an EST-Orestes library was constructed and characterized. As a result, 79 sequences were obtained, of which 39 (49.4%) had not been described previously in other libraries of this fungus and which could represent novel exclusive C-Y transition genes. Two of these sequences are, among others, cholestanol delta-isomerase, and electron transfer flavoprotein-ubiquinoneoxidoreductase (ETF-QO). The other 40 (50.6%) sequences were shared with Mycelia (M), Yeast (Y) or Mycelia to yest transition (M-Y) libraries. An important component of this group of sequences is a putative response regulator receiver SKN7, a protein of high importance in stress adaptation and a regulator of virulence in some bacteria and fungi. This is the first report identifying genes expressed during the C-Y transition process, the initial step required to understand the natural history of P. brasiliensis conidia induced infection.

• Autores: García Cepero, Ana María; Hernández Ruiz, Orville; Aristizabal Bernal, Beatriz Helena; De Souza Bernardes, Luciano Angelo; Puccia, Rosana; Naranjo Preciado, Tony Williams; Goldman, Gustavo; Goldman, María Helena; Cano Restrepo, Luz Elena; Restrepo Moreno, Ángela; McEwen Ochoa, Juan Guillermo
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2010
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/24133
• Acceso en línea: http://hdl.handle.net/10495/24133
• Palabra clave: Genes; Paracoccidioides; Esporas Fúngicas; Spores, Fungal
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc/2.5/co/