ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated From Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)

ABSTRACT: Within the Neoscytalidium genus, N. dimidiatum, N. oculus, N. orchidacearum, and N. novaehollandiae have been recognized. Although these species are frequently found in soil, N. dimidiatum has been identified as an etiologic agent of onychomycosis or dermatomycosis, and N. oculus has been...

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Autores:: Flórez Muñoz, Sindy Viviana
Mesa Arango, Ana Cecilia
Gómez Velásquez, Juan Carlos
Loaiza Giraldo, Ivett Natalia
Lima, Nelson
Santos, Carla
Soares, Célia

Tipo de recurso:: Article of investigation

Fecha de publicación:: 2019

Institución:: Universidad de Antioquia

Repositorio:: Repositorio UdeA

Idioma:: eng

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dc.title.spa.fl_str_mv	ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated From Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)
title	ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated From Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)
spellingShingle	ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated From Onychomycosis and Dermatomycosis Cases in Medellin (Colombia) Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción Dermatomycoses Dermatomicosis Onychomycosis Onicomicosis https://id.nlm.nih.gov/mesh/D019032 https://id.nlm.nih.gov/mesh/D003881 https://id.nlm.nih.gov/mesh/D014009
title_short	ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated From Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)
title_full	ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated From Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)
title_fullStr	ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated From Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)
title_full_unstemmed	ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated From Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)
title_sort	ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated From Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)
dc.creator.fl_str_mv	Flórez Muñoz, Sindy Viviana Mesa Arango, Ana Cecilia Gómez Velásquez, Juan Carlos Loaiza Giraldo, Ivett Natalia Lima, Nelson Santos, Carla Soares, Célia
dc.contributor.author.none.fl_str_mv	Flórez Muñoz, Sindy Viviana Mesa Arango, Ana Cecilia Gómez Velásquez, Juan Carlos Loaiza Giraldo, Ivett Natalia Lima, Nelson Santos, Carla Soares, Célia
dc.contributor.researchgroup.spa.fl_str_mv	GRID - Grupo de Investigación Dermatológica
dc.subject.decs.none.fl_str_mv	Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción Dermatomycoses Dermatomicosis Onychomycosis Onicomicosis
topic	Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción Dermatomycoses Dermatomicosis Onychomycosis Onicomicosis https://id.nlm.nih.gov/mesh/D019032 https://id.nlm.nih.gov/mesh/D003881 https://id.nlm.nih.gov/mesh/D014009
dc.subject.meshuri.none.fl_str_mv	https://id.nlm.nih.gov/mesh/D019032 https://id.nlm.nih.gov/mesh/D003881 https://id.nlm.nih.gov/mesh/D014009
description	ABSTRACT: Within the Neoscytalidium genus, N. dimidiatum, N. oculus, N. orchidacearum, and N. novaehollandiae have been recognized. Although these species are frequently found in soil, N. dimidiatum has been identified as an etiologic agent of onychomycosis or dermatomycosis, and N. oculus has been identified as an etiologic agent of an ocular lesion. All these species can be cultured in vitro, but their morphological identification by macroscopic and microscopic traits is difficult and imprecise due to their similarity. In this study, 34 isolates of Neoscytalidium spp. from 32 onychomycosis and two dermatomycosis cases in Medellin (Colombia) were identified at the species level using sequencing of the ITS1+5.8S+ITS2 nuclear rDNA region and MALDI-TOF mass spectrometry (MS). Neoscytalidium dimidiatum strain MUM 17.21 was used to construct the reference spectrum in the in-house library to identify the clinical isolates by MALDI-TOF MS. Additionally, N. dimidiatum PPC-216 and PLAB-055 strains were used to validate the in-house constructed reference spectra. Although four groups were observed in the dendrogram obtained from the proteins of each isolate profile, MALDI-TOF MS and sequencing results are in accordance, since all isolates were identified as N. dimidiatum.
publishDate	2019
dc.date.issued.none.fl_str_mv	2019
dc.date.accessioned.none.fl_str_mv	2024-06-26T17:48:44Z
dc.date.available.none.fl_str_mv	2024-06-26T17:48:44Z
dc.type.spa.fl_str_mv	Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv	Flórez-Muñoz SV, Gómez-Velásquez JC, Loaiza-Díaz N, Soares C, Santos C, Lima N, Mesa-Arango AC. ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated from Onychomycosis and Dermatomycosis Cases in Medellin (Colombia). Microorganisms. 2019 Sep 1;7(9):306. doi: 10.3390/microorganisms7090306.
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/10495/40309
dc.identifier.doi.none.fl_str_mv	10.3390/microorganisms7090306
dc.identifier.eissn.none.fl_str_mv	2076-2607
identifier_str_mv	Flórez-Muñoz SV, Gómez-Velásquez JC, Loaiza-Díaz N, Soares C, Santos C, Lima N, Mesa-Arango AC. ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated from Onychomycosis and Dermatomycosis Cases in Medellin (Colombia). Microorganisms. 2019 Sep 1;7(9):306. doi: 10.3390/microorganisms7090306. 10.3390/microorganisms7090306 2076-2607
url	https://hdl.handle.net/10495/40309
dc.language.iso.spa.fl_str_mv	eng
language	eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv	Microorganisms
dc.relation.citationendpage.spa.fl_str_mv	10
dc.relation.citationissue.spa.fl_str_mv	9
dc.relation.citationstartpage.spa.fl_str_mv	1
dc.relation.citationvolume.spa.fl_str_mv	7
dc.relation.ispartofjournal.spa.fl_str_mv	Microorganisms
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dc.format.extent.spa.fl_str_mv	10 páginas
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dc.publisher.place.spa.fl_str_mv	Basilea, Suiza
institution	Universidad de Antioquia
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spelling	Flórez Muñoz, Sindy VivianaMesa Arango, Ana CeciliaGómez Velásquez, Juan CarlosLoaiza Giraldo, Ivett NataliaLima, NelsonSantos, CarlaSoares, CéliaGRID - Grupo de Investigación Dermatológica2024-06-26T17:48:44Z2024-06-26T17:48:44Z2019Flórez-Muñoz SV, Gómez-Velásquez JC, Loaiza-Díaz N, Soares C, Santos C, Lima N, Mesa-Arango AC. ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated from Onychomycosis and Dermatomycosis Cases in Medellin (Colombia). Microorganisms. 2019 Sep 1;7(9):306. doi: 10.3390/microorganisms7090306.https://hdl.handle.net/10495/4030910.3390/microorganisms70903062076-2607ABSTRACT: Within the Neoscytalidium genus, N. dimidiatum, N. oculus, N. orchidacearum, and N. novaehollandiae have been recognized. Although these species are frequently found in soil, N. dimidiatum has been identified as an etiologic agent of onychomycosis or dermatomycosis, and N. oculus has been identified as an etiologic agent of an ocular lesion. All these species can be cultured in vitro, but their morphological identification by macroscopic and microscopic traits is difficult and imprecise due to their similarity. In this study, 34 isolates of Neoscytalidium spp. from 32 onychomycosis and two dermatomycosis cases in Medellin (Colombia) were identified at the species level using sequencing of the ITS1+5.8S+ITS2 nuclear rDNA region and MALDI-TOF mass spectrometry (MS). Neoscytalidium dimidiatum strain MUM 17.21 was used to construct the reference spectrum in the in-house library to identify the clinical isolates by MALDI-TOF MS. Additionally, N. dimidiatum PPC-216 and PLAB-055 strains were used to validate the in-house constructed reference spectra. Although four groups were observed in the dendrogram obtained from the proteins of each isolate profile, MALDI-TOF MS and sequencing results are in accordance, since all isolates were identified as N. dimidiatum.Universidad de Antioquia. Vicerrectoría de investigación. Comité para el Desarrollo de la Investigación - CODIFundação para a Ciência e TecnologiaBioTecNorte operationCOL005083910 páginasapplication/pdfengMDPIBasilea, Suizahttps://creativecommons.org/licenses/by/4.0/An error occurred getting the license - uri.http://creativecommons.org/licenses/by/2.5/co/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated From Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)Artículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationEspectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónDermatomycosesDermatomicosisOnychomycosisOnicomicosishttps://id.nlm.nih.gov/mesh/D019032https://id.nlm.nih.gov/mesh/D003881https://id.nlm.nih.gov/mesh/D014009Microorganisms10917Microorganisms2604UID/BIO/04469/2019NORTE-01-0145-FEDER-000004RoR:03bp5hc83RoR:00snfqn58PublicationORIGINALFlórezMuñozSindy_2019_Neoscytalidium_Botryosphaeriaceae_Onychomycosis.pdfFlórezMuñozSindy_2019_Neoscytalidium_Botryosphaeriaceae_Onychomycosis.pdfArtículo de investigaciónapplication/pdf1930646https://bibliotecadigital.udea.edu.co/bitstreams/24a6d248-b4d7-46bc-aa1f-9250d517f34f/download9ea7e91a2e84b46bc6e2879f2648b464MD51trueAnonymousREADCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated From Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)

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