Assessment of the Origins and Spread of Putative Resistance-Conferring Mutations in Plasmodium vivax Dihydropteroate Synthase

ABSTRACT: Infection with Plasmodium vivax is usually treated with chloroquine, but parasites are often exposed inadvertently to sulfadoxine-pyrimethamine. To infer patterns of selection and spread of resistant parasites in natural populations, we determined haplotypes of P. vivax dihydropteroate syn...

Full description

Autores:
Maestre Buitrago, Amanda Elena
Hawkins, Vivian N.
Suzuki, Stephanie M.
Rungsihirunrat, Kanchana
Hapuarachchi, Hapuarachchige C.
Na-Bangchang, Kesara
Hopkins Sibley, Carol
Tipo de recurso:
Article of investigation
Fecha de publicación:
2009
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/34373
Acceso en línea:
https://hdl.handle.net/10495/34373
https://doi.org/10.4269/ajtmh.2009.81.348
Palabra clave:
DNA, Intergenic
ADN Intergénico
Mutation
Mutación
Plasmodium vivax
Alleles
Alelos
Amino Acids
Aminoácidos
Dihydropteroate Synthase
Dihidropteroato Sintasa
Rights
openAccess
License
https://creativecommons.org/licenses/by/4.0/
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network_acronym_str UDEA2
network_name_str Repositorio UdeA
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dc.title.spa.fl_str_mv Assessment of the Origins and Spread of Putative Resistance-Conferring Mutations in Plasmodium vivax Dihydropteroate Synthase
title Assessment of the Origins and Spread of Putative Resistance-Conferring Mutations in Plasmodium vivax Dihydropteroate Synthase
spellingShingle Assessment of the Origins and Spread of Putative Resistance-Conferring Mutations in Plasmodium vivax Dihydropteroate Synthase
DNA, Intergenic
ADN Intergénico
Mutation
Mutación
Plasmodium vivax
Alleles
Alelos
Amino Acids
Aminoácidos
Dihydropteroate Synthase
Dihidropteroato Sintasa
title_short Assessment of the Origins and Spread of Putative Resistance-Conferring Mutations in Plasmodium vivax Dihydropteroate Synthase
title_full Assessment of the Origins and Spread of Putative Resistance-Conferring Mutations in Plasmodium vivax Dihydropteroate Synthase
title_fullStr Assessment of the Origins and Spread of Putative Resistance-Conferring Mutations in Plasmodium vivax Dihydropteroate Synthase
title_full_unstemmed Assessment of the Origins and Spread of Putative Resistance-Conferring Mutations in Plasmodium vivax Dihydropteroate Synthase
title_sort Assessment of the Origins and Spread of Putative Resistance-Conferring Mutations in Plasmodium vivax Dihydropteroate Synthase
dc.creator.fl_str_mv Maestre Buitrago, Amanda Elena
Hawkins, Vivian N.
Suzuki, Stephanie M.
Rungsihirunrat, Kanchana
Hapuarachchi, Hapuarachchige C.
Na-Bangchang, Kesara
Hopkins Sibley, Carol
dc.contributor.author.none.fl_str_mv Maestre Buitrago, Amanda Elena
Hawkins, Vivian N.
Suzuki, Stephanie M.
Rungsihirunrat, Kanchana
Hapuarachchi, Hapuarachchige C.
Na-Bangchang, Kesara
Hopkins Sibley, Carol
dc.contributor.researchgroup.spa.fl_str_mv Salud y Comunidad
dc.subject.decs.none.fl_str_mv DNA, Intergenic
ADN Intergénico
Mutation
Mutación
Plasmodium vivax
Alleles
Alelos
Amino Acids
Aminoácidos
Dihydropteroate Synthase
Dihidropteroato Sintasa
topic DNA, Intergenic
ADN Intergénico
Mutation
Mutación
Plasmodium vivax
Alleles
Alelos
Amino Acids
Aminoácidos
Dihydropteroate Synthase
Dihidropteroato Sintasa
description ABSTRACT: Infection with Plasmodium vivax is usually treated with chloroquine, but parasites are often exposed inadvertently to sulfadoxine-pyrimethamine. To infer patterns of selection and spread of resistant parasites in natural populations, we determined haplotypes of P. vivax dihydropteroate synthase ( dhps ) alleles that could confer resistance to sulfadoxine. We amplified the P. vivax pyrophosphokinase ( pppk )– dhps region and its flanking intergenic regions from 92 contemporary global isolates. Introns and exons of pppk-dhps were highly polymorphic, as were the flanking intergenic regions. Eighteen haplotypes were associated with wild-type alleles, but several different putatively sulfadoxine-resistant alleles have arisen in areas of intensive sulfadoxine-pyrimethamine use. Even when they encoded changes to the same amino acid, these mutant alleles were associated with multiple different haplotypes. Two main conclusions can be drawn from these data. First, dhps alleles resistant to sulfadoxine have arisen multiple times under drug pressure. Second, there has been convergent evolution of a variety of alleles that could confer resistance to sulfa drugs.
publishDate 2009
dc.date.issued.none.fl_str_mv 2009
dc.date.accessioned.none.fl_str_mv 2023-03-30T22:13:26Z
dc.date.available.none.fl_str_mv 2023-03-30T22:13:26Z
dc.type.spa.fl_str_mv Artículo de investigación
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
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dc.identifier.issn.none.fl_str_mv 0002-9637
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10495/34373
dc.identifier.doi.none.fl_str_mv 10.4269/ajtmh.2009.81.348
dc.identifier.eissn.none.fl_str_mv 1476-1645
dc.identifier.url.spa.fl_str_mv https://doi.org/10.4269/ajtmh.2009.81.348
identifier_str_mv 0002-9637
10.4269/ajtmh.2009.81.348
1476-1645
url https://hdl.handle.net/10495/34373
https://doi.org/10.4269/ajtmh.2009.81.348
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Am. J. Trop. Med. Hyg.
dc.relation.citationendpage.spa.fl_str_mv 355
dc.relation.citationissue.spa.fl_str_mv 2
dc.relation.citationstartpage.spa.fl_str_mv 348
dc.relation.citationvolume.spa.fl_str_mv 81
dc.relation.ispartofjournal.spa.fl_str_mv American Journal of Tropical Medicine and Hygiene
dc.rights.uri.spa.fl_str_mv https://creativecommons.org/licenses/by/4.0/
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dc.publisher.spa.fl_str_mv American Society of Tropical Medicine and Hygiene
dc.publisher.place.spa.fl_str_mv Baltimore, Estados Unidos
dc.publisher.faculty.spa.fl_str_mv sin facultad - programa
institution Universidad de Antioquia
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spelling Maestre Buitrago, Amanda ElenaHawkins, Vivian N.Suzuki, Stephanie M.Rungsihirunrat, KanchanaHapuarachchi, Hapuarachchige C.Na-Bangchang, KesaraHopkins Sibley, CarolSalud y Comunidad2023-03-30T22:13:26Z2023-03-30T22:13:26Z20090002-9637https://hdl.handle.net/10495/3437310.4269/ajtmh.2009.81.3481476-1645https://doi.org/10.4269/ajtmh.2009.81.348ABSTRACT: Infection with Plasmodium vivax is usually treated with chloroquine, but parasites are often exposed inadvertently to sulfadoxine-pyrimethamine. To infer patterns of selection and spread of resistant parasites in natural populations, we determined haplotypes of P. vivax dihydropteroate synthase ( dhps ) alleles that could confer resistance to sulfadoxine. We amplified the P. vivax pyrophosphokinase ( pppk )– dhps region and its flanking intergenic regions from 92 contemporary global isolates. Introns and exons of pppk-dhps were highly polymorphic, as were the flanking intergenic regions. Eighteen haplotypes were associated with wild-type alleles, but several different putatively sulfadoxine-resistant alleles have arisen in areas of intensive sulfadoxine-pyrimethamine use. Even when they encoded changes to the same amino acid, these mutant alleles were associated with multiple different haplotypes. Two main conclusions can be drawn from these data. First, dhps alleles resistant to sulfadoxine have arisen multiple times under drug pressure. Second, there has been convergent evolution of a variety of alleles that could confer resistance to sulfa drugs.COL00474498application/pdfengAmerican Society of Tropical Medicine and HygieneBaltimore, Estados Unidossin facultad - programahttps://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/2.5/co/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Assessment of the Origins and Spread of Putative Resistance-Conferring Mutations in Plasmodium vivax Dihydropteroate SynthaseArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionDNA, IntergenicADN IntergénicoMutationMutaciónPlasmodium vivaxAllelesAlelosAmino AcidsAminoácidosDihydropteroate SynthaseDihidropteroato SintasaAm. J. Trop. Med. 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